Month: March 2022

SDS of cas: 70539-42-3. Aromatic compounds can be divided into two categories: single heterocycles and fused heterocycles. Compound: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, is researched, Molecular C18H20N2O12, CAS is 70539-42-3, about A high throughput dimer screening assay for monoclonal antibodies using chemical cross-linking and microchip electrophoresis. Author is Chen, Xiaoyu; Flynn, Gregory C..

A high throughput screening assay was developed to determine the total dimer level in antibody samples. This method utilizes high speed microchip electrophoresis separation following chem. crosslinking. Upon reacting with homobifunctional N-hydroxysuccinimide-esters (NHS-esters), covalent linkages can be established between the primary amines of two neighboring antibody mols. The reaction conditions are optimized to achieve quant. crosslinking of only phys. associated monomers within an antibody dimer. The resulting crosslinked dimers, originating from either covalent or non-covalent antibody dimers, can then be separated from monomers by SDS electrophoresis. A com. microchip electrophoresis instrument is used for high speed separation, allowing each sample to be analyzed in about 1 min. This approach was applied to crude mammalian cell culture samples. Using a 96-well gel filtration spin column format, interfering species in the cell culture media were efficiently removed from the samples. This method is well suited to the purpose of high throughput antibody dimer quantitation during cell culture expression, including clone selection and cell culture development. The total dimer content, both covalent and non-covalent, can be determined for hundreds of crude samples in a few hours. The effects of different crosslinking conditions on the determined dimer levels, as well as of different antibody pI values, are discussed.

Here is a brief introduction to this compound(70539-42-3)SDS of cas: 70539-42-3, if you want to know about other compounds related to this compound(70539-42-3), you can read my other articles.

Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

The reaction of an aromatic heterocycle with a proton is called a protonation. One of articles about this theory is 《Sequence of release of neurohormones on nervous stimulation of frog’s stomach muscle》. Authors are Singh, I..The article about the compound:Sodium 4-butyl-3,5-dioxo-1,2-diphenylpyrazolidin-4-idecas:129-18-0,SMILESS:O=C(N(C1=CC=CC=C1)N2C3=CC=CC=C3)[C-](CCCC)C2=O.[Na+]).COA of Formula: C19H19N2NaO2. Through the article, more information about this compound (cas:129-18-0) is conveyed.

The experiments were performed on the stomach muscle of Rana tigrina. The nerve-muscle preparations were made as described by S., et al. (CA 56, 9285e), and were immersed in saline, or in 0.112M sucrose. The contraction produced by nerve stimulation showed seasonal variations. In summer (room temperature 29-31°), the latent period of response was 2-5 sec., and in winter (room temperature 23-5°), the latent period was 20-50 sec. In winter and in summer, atropine sulfate (I) did not abolish the contraction, unless it was previously treated with 2-bromo-D-lysergic acid diethylamide (II). Neopyramine (III) did not abolish the contraction of untreated muscles, but it abolished the contraction of muscles treated with I and II. The effects of II are different in summer and in winter. In winter, the contraction were diminished or abolished. In summer, the contractions were enhanced, but if the muscles were soaked in sucrose solution, the effect in summer was similar to that in winter. A combination of I, II, and III did not affect, or slightly diminished contractions in summer and in winter. If the muscle is soaked in sucrose, the contraction persists, indicating a release of substance P. Substance P itself caused contraction in sucrose-soaked muscles. As in winter, nervous stimulation in summer also produced relaxation, which was abolished by II the relaxation produced by adrenaline or noradrenaline was not affected.

Here is a brief introduction to this compound(129-18-0)COA of Formula: C19H19N2NaO2, if you want to know about other compounds related to this compound(129-18-0), you can read my other articles.

Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

In organic chemistry, atoms other than carbon and hydrogen are generally referred to as heteroatoms. The most common heteroatoms are nitrogen, oxygen and sulfur. Now I present to you an article called Covalent labeling of vasopressin receptors from LLC-PK1 cells by the use of a bifunctional reagent, published in 1988-04-30, which mentions a compound: 70539-42-3, mainly applied to ethyleneglycolsuccinimidyl succinate vasopressin receptor complexation, Category: benzofurans.

The possibility of covalently attaching vasopressin to its receptors by the use of a bifunctional reagent was explored. Plasma membranes from the LLC-PK1 pig kidney cell line were purified by Percoll d. gradient centrifugation. These membranes contained a single population of high affinity (dissociation constant = 5.2 nM) and high maximum binding capacity (3.8 pmol/mg protein) [3H]lysine vasopressin ([3H]LVP)-binding sites. [3H]LVP-labeled receptors were solubilized with a high yield (83%) and minimal dissociation (9%) by treatment with the nonionic detergent, octaethylene glycol monododecyl ether (0.5%) in the presence of glycerol (20%). The solubilized [3H]LVP-labeled receptors were stable upon storage at 4° (5% dissociation after 24 h). They were partially purified to a specific activity of 17 pmol/mg of protein by chromatog. on a Cibacron blue-Sepharose column with a yield of 90%. The [3H]LVP-receptor complexes in both intact membranes and the partially purified preparation were almost completely dissociated by incubation at 30° for 30 min in the presence of 20 mM EDTA. This property was used to test the effect of ethylene glycol bis(succinimidyl-succinate) (EGS) as crosslinking reagent for the covalent attachment of [3H]LVP to its receptors. After treatment of [3H]LVP-labeled membranes for 30 min with 1 mM EGS at 4°, about 30% of specifically bound [3H]LVP was resistant to EDTA dissociation The amount of EDTA-resistant binding varied as a linear function of the fractional receptor occupancy and maximal binding capacity of the different batches of membranes used. Similar results were obtained with solubilized and partially purified vasopressin receptors. Upon steric exclusion HPLC, the EDTA-resistant [3H]LVP-labeled material, like the native [3H]LVP-labeled receptor, was eluted as a single and apparently homogeneous peak. The covalent character of the EGS-induced [3H]LVP binding to solubilized or partially purified receptors was assessed by its resistance to SDS-PAGE. The yield of EGS-induced labeling deduced from these experiments (27%) was close to that determined by the EDTA method. SDS-PAGE anal. of the [3H]LVP-labeled cross-linked material revealed the specific labeling of a major 50 kilodalton (kDa) component and a minor component of 30 kDa. The size of these 2 components was not affected by dithiothreitol.

Here is a brief introduction to this compound(70539-42-3)Category: benzofurans, if you want to know about other compounds related to this compound(70539-42-3), you can read my other articles.

Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

In organic chemistry, atoms other than carbon and hydrogen are generally referred to as heteroatoms. The most common heteroatoms are nitrogen, oxygen and sulfur. Now I present to you an article called Synergism between acid and gastric contractile activity in the genesis of ulceration and hemorrhage in the phenylbutazone-treated rat, published in 1981-09-30, which mentions a compound: 129-18-0, mainly applied to phenylbutazone stomach ulcer mechanism, Name: Sodium 4-butyl-3,5-dioxo-1,2-diphenylpyrazolidin-4-ide.

The nonsteroid anti-inflammatory drugs including phenylbutazone produce significant gastric ulceration and hemorrhage even when administered by an extragastric route. To investigate the mechanism involved, cervical sectioned rats received an intraileal injection of phenylbutazone Na (I Na) [129-18-0] in methylcellulose. Gastric contractile activity was recorded with a miniature gastric balloon, and the stomach was perfused with either acid at pH 1 or buffer at pH 7. Rats receiving I developed a pattern of abnormally strong contractile activity that began 60-90 min after drug administration. The contracting stomachs of rats perfused with acid developed more ulceration and bled more severely than buffer-perfused rats. Vagotomy or atropine prevented the gastric contractile response to I, and such rats failed to develop significant ulceration in spite of the fact that their stomachs were perfused with acid. Cervical sectioned rats treated with methylcellulose alone failed to develop the abnormal contractile pattern and showed no significant gastric injury or hemorrhage. Thus, the focal hemorrhagic lesions induced by the extragastric administration of I in the rat are the result of mucosal compression at specific sites secondary to the passage of extremely strong peristaltic waves. They are vagally mediated and although the presence of acid does not appear essential for their initiation, it plays a synergistic role in their development and hemorrhage.

Here is a brief introduction to this compound(129-18-0)Name: Sodium 4-butyl-3,5-dioxo-1,2-diphenylpyrazolidin-4-ide, if you want to know about other compounds related to this compound(129-18-0), you can read my other articles.

Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Category: benzofurans. The fused heterocycle is formed by combining a benzene ring with a single heterocycle, or two or more single heterocycles. Compound: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, is researched, Molecular C18H20N2O12, CAS is 70539-42-3, about Affinity labeling of the Fc receptor on human monocytes using bifunctional cross-linking agents. Author is Kurlander, Roger; Niedel, James.

To affinity label the Fc receptor on human monocytes, Fc fragments of monoclonal human IgG1 radiolabeled with 125I were covalently bound to the surface of intact monocytes using a variety of bifunctional cross-linking agents including ethylene glycol bis(succinimidyl succinate), dithio-bis-(succinimidyl proprionate), maleimidobenzoyl N-hydroxysuccinimide, glutaraldehyde, and di-Me suberimidate. After cross-linking, cells were solubilized and subjected to SDS-polyacrylamide gel electrophoresis, followed by radioautog. Each of these cross-linkers caused a portion of cell-bound Fc fragments to form a covalent complex with a monocyte membrane component. This complex migrated on electrophoresis with an apparent mol. weight of 120,000. Deducting the mol. weight of Fc fragments alone (53,000) the mol. weight of the second component of the complex therefore was about 67,000. A similar estimate of receptor size also was obtained after reduction with dithiothreitol. Complex formation was potently inhibited by unlabeled Fc fragments, IgG1 or IgG3, all of which would be expected to compete with Fc fragments for IgG Fc receptor on human monocytes; it was not inhibited by Fab fragments, IgG2 or IgG4, which do not bind avidly to this receptor. Complex formation was saturable, and Fc fragments formed complexes with avidity comparable to that with which Fc fragments bound to receptors on intact monocytes. The findings establish the feasibility of using radiolabeled Fc fragments to affinity label the IgG Fc receptors on human leukocytes. Potential advantages of this approach to studying receptor structure are discussed.

Here is a brief introduction to this compound(70539-42-3)Category: benzofurans, if you want to know about other compounds related to this compound(70539-42-3), you can read my other articles.

Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Quality Control of (R)-Ethyl 4-chloro-3-hydroxybutanoate. The reaction of aromatic heterocyclic molecules with protons is called protonation. Aromatic heterocycles are more basic than benzene due to the participation of heteroatoms. Compound: (R)-Ethyl 4-chloro-3-hydroxybutanoate, is researched, Molecular C6H11ClO3, CAS is 90866-33-4, about Synthesis of ethyl (R)-4-chloro-3-hydroxybutyrate by immobilized cells using amino acid-modified magnetic nanoparticles. Author is Lu, Yuan; Dai, Hongqian; Shi, Hanbing; Tang, Lan; Sun, Xingyuan; Ou, Zhimin.

Fe3O4-Arg was selected as the optimal carrier due to its high activity recovery of immobilized cells in the preparation of Fe3O4-Arg-Cells. The optimal immobilization conditions for the preparation of Fe3O4-Arg-Cells were 30°C, 4 h, pH 7, and 3 g dry yeast. The activity recovery of immobilized cells reached 76.8%. For a batch reduction in a shaker in an alternating magnetic field, Fe3O4-Arg-Cells were used as a catalyst to gain Et (R)-4-chloro-3-hydroxybutyrate ((R)-CHBE). For further improvement in reduction productivity, a continuous reduction in the magnetic fluidized bed reactor system (MFBRS) was completed. Under their optimal transformation conditions, it took 24 h for Fe3O4-Arg-Cells to complete the conversion of Et 4-chloro-3-oxobutanoate (COBE) (0.8553 mol/L) in the shaker and only 8 h for the batch reduction in an alternating magnetic field. Continuous reduction in MFBRS provided new ideas for the efficient production of (R)-CHBE; 1.5882 mol/L (10 mL) of COBE can be completely converted in 6 h. The conversion and enantiomeric excess (e.e.) of (R)-CHBE were 100% and above 99.9% resp., in the three reaction systems mentioned above.

Here is a brief introduction to this compound(90866-33-4)Quality Control of (R)-Ethyl 4-chloro-3-hydroxybutanoate, if you want to know about other compounds related to this compound(90866-33-4), you can read my other articles.

Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Application of 90866-33-4. So far, in addition to halogen atoms, other non-metallic atoms can become part of the aromatic heterocycle, and the target ring system is still aromatic. Compound: (R)-Ethyl 4-chloro-3-hydroxybutanoate, is researched, Molecular C6H11ClO3, CAS is 90866-33-4, about Stereoselective reduction of ethyl 4-chloro-3-oxobutanoate by Escherichia coli transformant cells coexpressing the aldehyde reductase and glucose dehydrogenase genes.

The asym. reduction of Et 4-chloro-3-oxobutanoate to Et (R)-4-chloro-3-hydroxybutanoate (I) using E. coli cells, which coexpress both the aldehyde reductase gene from Sporobolomyces salmonicolor and the glucose dehydrogenase (GDH) gene from Bacillus megaterium as a catalyst was investigated. In an organic solvent-water 2-phase system, I formed in the organic phase amounted to 1610 mM (268 mg/mL), with a molar yield of 94.1% and an optical purity of 91.7% e.e. The calculated turnover number of NADP+ to I formed was 13,500 mol/mol. Since the use of E. coli JM109 cells harboring pKAR and pACGD as a catalyst is simple and does not require the addition of GDH or the isolation of the enzymes, it is highly advantageous for the practical synthesis of I.

Here is a brief introduction to this compound(90866-33-4)Application of 90866-33-4, if you want to know about other compounds related to this compound(90866-33-4), you can read my other articles.

Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Pawelczyk, Ewaryst; Wachowiak, Roman published an article about the compound: Sodium 4-butyl-3,5-dioxo-1,2-diphenylpyrazolidin-4-ide( cas:129-18-0,SMILESS:O=C(N(C1=CC=CC=C1)N2C3=CC=CC=C3)[C-](CCCC)C2=O.[Na+] ).Quality Control of Sodium 4-butyl-3,5-dioxo-1,2-diphenylpyrazolidin-4-ide. Aromatic heterocyclic compounds can be classified according to the number of heteroatoms or the size of the ring. The authors also want to convey more information about this compound (cas:129-18-0) through the article.

The decomposition products formed when 20% aqueous alk. solutions of phenylbutazone Na salt were aged in sunlight were isolated and identified. The colored decomposition products were isolated by CHCl3 extraction; the colorless products were extracted with ether from the acidified solution The extracts were concentrated and separated by thin-layer chromatog. The 5 decomposition products isolated and identified with Ce(SO4)2 were (Rfvalue given) 4-hydroxyphenylbutazone (0.33), N-(2-carboxycaproyl)-hydrazobenzene (0.1), N-(2-carboxy-2-hydroxycaproyl)hydrazobenzene (0.55), trans-azobenzene (0.78), and cis-azobenzene (0.62).

Here is a brief introduction to this compound(129-18-0)Quality Control of Sodium 4-butyl-3,5-dioxo-1,2-diphenylpyrazolidin-4-ide, if you want to know about other compounds related to this compound(129-18-0), you can read my other articles.

Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

The three-dimensional configuration of the ester heterocycle is basically the same as that of the carbocycle. Compound: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate(SMILESS: O=C(ON1C(CCC1=O)=O)CCC(OCCOC(CCC(ON2C(CCC2=O)=O)=O)=O)=O,cas:70539-42-3) is researched.Application of 19777-66-3. The article 《The use of an in vitro sperm activation assay to detect chemically induced damage of human sperm nuclei》 in relation to this compound, is published in Reproductive Toxicology. Let’s take a look at the latest research on this compound (cas:70539-42-3).

We report that human sperm chem. damaged in vitro by treatment with a reversible crosslinker, ethylene glycol bis(sulfosuccinimidylsuccinate; SEGS), display abnormal chromatin decondensation when analyzed in the human sperm activation assay (HSAA). Less than 20% of SEGS-treated sperm fully decondensed, vs. 97% of control sperm. Chem. reversal of the crosslinks by treatment with 5 μM hydroxylamine restored full decondensation in 76% of treated sperm. These results demonstrate that chem. damaged sperm respond abnormally in the HSAA, and that chem. damage to sperm nuclei can be detected using the HSAA. Thus, there is potential for the HSAA to be used to detect chem. alterations of sperm nuclei from men exposed to environmental toxicants.

Here is a brief introduction to this compound(70539-42-3)Safety of Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, if you want to know about other compounds related to this compound(70539-42-3), you can read my other articles.

Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Heterocyclic compounds can be divided into two categories: alicyclic heterocycles and aromatic heterocycles. Compounds whose heterocycles in the molecular skeleton cannot reflect aromaticity are called alicyclic heterocyclic compounds. Compound: 2923-28-6, is researched, Molecular CAgF3O3S, about Structure of copper(I) and silver(I) complexes with zwitterionic ligands derived from N-Heterocyclic Carbenes, the main research direction is copper silver bisdiisopropylphenylimidazolylidene complex preparation; crystal structure copper silver bisdiisopropylphenylimidazolylidene complex.Electric Literature of CAgF3O3S.

N-Heterocyclic Carbene (NHC) ligands have been instrumental in the synthesis of novel metal and main group-containing compounds, as well as their subsequent reactivity. Here, the coordination chem. of CS2 and PhNCS derivatives of the sterically encumbering 1,3-bis(2,6-diisopropylphenyl)imidazol-2-ylidene (IPr) are studied. The reaction of IPr•CS2 with Cu(I) and Ag(I) salts results in the isolation of dinuclear complexes, [(IPr•CS2)2M2(THF)n][X]2, M = Cu, X = PF6, n = 4; M = Ag, X = OTf, n = 2. The 1:1 stoichiometric reaction of IPr•PhNCS with CuI results in to the formation of [(IPr•PhNCS)2Cu][CuI2]. All complexes were characterized by 1H, 13C, and IR spectroscopy and their solid-state structures determined by X-ray crystallog.

Here is a brief introduction to this compound(2923-28-6)Electric Literature of CAgF3O3S, if you want to know about other compounds related to this compound(2923-28-6), you can read my other articles.

Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem