Category: benzofurans. The fused heterocycle is formed by combining a benzene ring with a single heterocycle, or two or more single heterocycles. Compound: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, is researched, Molecular C18H20N2O12, CAS is 70539-42-3, about Affinity labeling of the Fc receptor on human monocytes using bifunctional cross-linking agents. Author is Kurlander, Roger; Niedel, James.
To affinity label the Fc receptor on human monocytes, Fc fragments of monoclonal human IgG1 radiolabeled with 125I were covalently bound to the surface of intact monocytes using a variety of bifunctional cross-linking agents including ethylene glycol bis(succinimidyl succinate), dithio-bis-(succinimidyl proprionate), maleimidobenzoyl N-hydroxysuccinimide, glutaraldehyde, and di-Me suberimidate. After cross-linking, cells were solubilized and subjected to SDS-polyacrylamide gel electrophoresis, followed by radioautog. Each of these cross-linkers caused a portion of cell-bound Fc fragments to form a covalent complex with a monocyte membrane component. This complex migrated on electrophoresis with an apparent mol. weight of 120,000. Deducting the mol. weight of Fc fragments alone (53,000) the mol. weight of the second component of the complex therefore was about 67,000. A similar estimate of receptor size also was obtained after reduction with dithiothreitol. Complex formation was potently inhibited by unlabeled Fc fragments, IgG1 or IgG3, all of which would be expected to compete with Fc fragments for IgG Fc receptor on human monocytes; it was not inhibited by Fab fragments, IgG2 or IgG4, which do not bind avidly to this receptor. Complex formation was saturable, and Fc fragments formed complexes with avidity comparable to that with which Fc fragments bound to receptors on intact monocytes. The findings establish the feasibility of using radiolabeled Fc fragments to affinity label the IgG Fc receptors on human leukocytes. Potential advantages of this approach to studying receptor structure are discussed.
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Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem