Derivation of elementary reaction about 70539-42-3

《Increasing the Depth of Mass-Spectrometry-Based Structural Analysis of Protein Complexes through the Use of Multiple Cross-Linkers》 provides a strategy for the preparation of materials with excellent comprehensive properties, which is conducive to broaden the application field of this compound(Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate)Synthetic Route of C18H20N2O12.

Most of the compounds have physiologically active properties, and their biological properties are often attributed to the heteroatoms contained in their molecules, and most of these heteroatoms also appear in cyclic structures. A Journal, Article, Research Support, Non-U.S. Gov’t, Analytical Chemistry (Washington, DC, United States) called Increasing the Depth of Mass-Spectrometry-Based Structural Analysis of Protein Complexes through the Use of Multiple Cross-Linkers, Author is Ding, Yue-He; Fan, Sheng-Bo; Li, Shuang; Feng, Bo-Ya; Gao, Ning; Ye, Keqiong; He, Si-Min; Dong, Meng-Qiu, which mentions a compound: 70539-42-3, SMILESS is O=C(ON1C(CCC1=O)=O)CCC(OCCOC(CCC(ON2C(CCC2=O)=O)=O)=O)=O, Molecular C18H20N2O12, Synthetic Route of C18H20N2O12.

Chem. crosslinking of proteins coupled with mass spectrometry (CXMS) is a powerful tool to study protein folding and to map the interfaces between interacting proteins. The most commonly used cross-linkers in CXMS are BS3 and DSS, which have similar structures and generate the same linkages between pairs of lysine residues in spatial proximity. However, there are cases where no cross-linkable lysine pairs are present at certain regions of a protein or at the interface of two interacting proteins. In order to find the cross-linkers that can best complement the performance of BS3 and DSS, we tested seven addnl. cross-linkers that either have different spacer arm structures or that target different amino acids (BS2G, EGS, AMAS, GMBS, Sulfo-GMBS, EDC, and TFCS). Using BSA, aldolase, the yeast H/ACA protein complex, and E. coli 70S ribosomes, we showed that, in terms of providing structural information not obtained through the use of BS3 and DSS, EGS and Sulfo-GMBS worked better than the other cross-linkers that we tested. EGS generated a large number of cross-links not seen with the other amine-specific cross-linkers, possibly due to its hydrophilic spacer arm. We demonstrate that incorporating the cross-links contributed by the EGS and amine-sulfhydryl cross-linkers greatly increased the accuracy of Rosetta in docking the structure of the yeast H/ACA protein complex. Given the improved depth of useful information it can provide, we suggest that the multilinker CXMS approach should be used routinely when the amount of a sample permits.

《Increasing the Depth of Mass-Spectrometry-Based Structural Analysis of Protein Complexes through the Use of Multiple Cross-Linkers》 provides a strategy for the preparation of materials with excellent comprehensive properties, which is conducive to broaden the application field of this compound(Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate)Synthetic Route of C18H20N2O12.

Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

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Different reactions of this compound(Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate)Recommanded Product: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate require different conditions, so the reaction conditions are very important.

Heterocyclic compounds can be divided into two categories: alicyclic heterocycles and aromatic heterocycles. Compounds whose heterocycles in the molecular skeleton cannot reflect aromaticity are called alicyclic heterocyclic compounds. Compound: 70539-42-3, is researched, Molecular C18H20N2O12, about Identification of a receptor for peptides of the bombesin family in Swiss 3T3 cells by affinity cross-linking, the main research direction is receptor bombesin peptide 3T3 cell; affinity crosslinking bombesin receptor.Recommanded Product: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate.

The cross-linking agent ethylene glycolbis(succinimidyl succinate) was used to covalently link 125I-labeled gastrin-releasing peptide (125I-GRP) [80043-53-4] to a 75,000-85,000-mol.-weight surface protein in Swiss 3T3 cells that displays many characteristics of a specific receptor for peptides of the bombesin family. This protein was not present in other cell lines which do not exhibit receptors for bombesin-like peptides. Unlabeled GRP competed for affinity labeling of the 75,000-85,000-mol.-weight protein in a concentration-dependent manner, and other bombesin-related peptides also inhibited the cross-linking of 125I-GRP to this component. In contrast, high concentrations of a variety of other peptide hormones and mitogens had no effect. Affinity labeling of the 75,000-85,000-mol.-weight protein was dependent on the concentration of 125I-GRP and exhibited saturability. 125I-GRP affinity labeling of this protein was also demonstrated by 2-dimensional gel electrophoresis. These studies suggest that an 75,000-85,000-mol.-weight surface protein with an isoelec. point of 6.0-6.5 is a major component of the receptor for peptides of the bombesin family in Swiss 3T3 cells.

Different reactions of this compound(Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate)Recommanded Product: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate require different conditions, so the reaction conditions are very important.

Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Chemical Properties and Facts of 70539-42-3

Different reactions of this compound(Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate)Application of 70539-42-3 require different conditions, so the reaction conditions are very important.

The preparation of ester heterocycles mostly uses heteroatoms as nucleophilic sites, which are achieved by intramolecular substitution or addition reactions. Compound: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate( cas:70539-42-3 ) is researched.Application of 70539-42-3.Massague, Joan; Guillette, Barbara J.; Czech, Michael P. published the article 《Affinity labeling of multiplication stimulating activity receptors in membranes from rat and human tissues》 about this compound( cas:70539-42-3 ) in Journal of Biological Chemistry. Keywords: multiplication stimulating activity receptor; membrane multiplication stimulating activity receptor; adipose multiplication stimulating activity receptor; placenta multiplication stimulating activity receptor; liver multiplication stimulating activity receptor. Let’s learn more about this compound (cas:70539-42-3).

Plasma membranes from rat adipocytes and liver and from human placenta were labeled by covalent crosslinking to membrane-bound 125I-labeled multiplication-stimulating activity (125I-MSA) with 3 different bishydroxysuccinimide esters: disuccinimidyl suberate, disuccinimidyl succinate, and ethyleneglycolylbis(succinimidyl succinate). Dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiog. anal. of the 125I-MSA-labeled material in the presence of dithiothreitol reveals 1 single-labeled protein migrating with an apparent Mr = 255,000 regardless of the kind and concentration of crosslinker used. Electrophoresis in the absence of reductant indicates that the affinity-labeled species is not disulfide-linked to any other protein in the native plasma membrane, but contains internal disulfide bonds that compact its structure. The labeling of the Mr = 255,000 species increases with increasing concentrations of 125I-MSA of 0.3-3 nM. Labeling is abolished in a competitive manner by nonradioactive MSA but not by similar concentrations of insulin, proinsulin, or epidermal growth factor in all 3 tissues examined The unique labeling of this Mr = 225,000 membrane component and its selective inhibition by MSA suggest that this protein is a plasma membrane receptor for MSA.

Different reactions of this compound(Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate)Application of 70539-42-3 require different conditions, so the reaction conditions are very important.

Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Simple exploration of 70539-42-3

Different reactions of this compound(Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate)Application of 70539-42-3 require different conditions, so the reaction conditions are very important.

The three-dimensional configuration of the ester heterocycle is basically the same as that of the carbocycle. Compound: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate(SMILESS: O=C(ON1C(CCC1=O)=O)CCC(OCCOC(CCC(ON2C(CCC2=O)=O)=O)=O)=O,cas:70539-42-3) is researched.Application of 70539-42-3. The article 《Cleavable ester-linked magnetic nanoparticles for labeling of solvent-exposed primary amine groups of peptides/proteins》 in relation to this compound, is published in Analytical Biochemistry. Let’s take a look at the latest research on this compound (cas:70539-42-3).

To study the solvent-exposed lysine residues of peptides/proteins, we previously reported disulfide-linked N-hydroxysuccinimide ester-modified silica-coated iron oxide magnetic nanoparticles (NHS-SS-SiO2@Fe3O4 MNPs). The presence of a disulfide bond in the linker limits the use of disulfide reducing agent during protein digestion and allows unwanted disulfide formation between the MNPs and protein. In the current work, the disulfide bond was replaced with a cleavable ester group to synthesize NHS ester-modified SiO2@Fe3O4 MNPs. Use of the cleavable ester group provides an improved method for protein labeling and allows the use of disulfide reducing agents during protein digestion.

Different reactions of this compound(Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate)Application of 70539-42-3 require different conditions, so the reaction conditions are very important.

Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

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Different reactions of this compound(Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate)Category: benzofurans require different conditions, so the reaction conditions are very important.

Category: benzofurans. The reaction of aromatic heterocyclic molecules with protons is called protonation. Aromatic heterocycles are more basic than benzene due to the participation of heteroatoms. Compound: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, is researched, Molecular C18H20N2O12, CAS is 70539-42-3, about The stability and functionality of chemically crosslinked microtubules. Author is Boal, Andrew K.; Tellez, Hernesto; Rivera, Susan B.; Miller, Nicholas E.; Bachand, George D.; Bunker, Bruce C..

A variety of bifunctional crosslinking agents have been explored for stabilizing microtubule shuttles used for the active transport of nanomaterials in artificial environments. Crosslinking agents that target amine residues form intertubulin crosslinks that produce crosslinked microtubules (CLMTs) with structural and functional lifetimes that can be up to four times as long as those achieved with taxol stabilization. Such CLMTs are stable at temperatures down to -10°C, are resistant to depolymerization induced by metal ions such as Ca2+, and yet continue to be adsorbed and transported by self-assembled monolayers containing the motor protein kinesin. However, crosslinkers that target cysteine residues depolymerize the MTs, probably by interfering with the guanosine triphosphate binding site. The impact of crosslink attributes, including terminal group chem., chain length, crosslink d., and specific location on the tubulin surface, on microtubule stability and functionality are discussed.

Different reactions of this compound(Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate)Category: benzofurans require different conditions, so the reaction conditions are very important.

Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

The Absolute Best Science Experiment for 70539-42-3

Different reactions of this compound(Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate)Formula: C18H20N2O12 require different conditions, so the reaction conditions are very important.

Most of the natural products isolated at present are heterocyclic compounds, so heterocyclic compounds occupy an important position in the research of organic chemistry. A compound: 70539-42-3, is researched, SMILESS is O=C(ON1C(CCC1=O)=O)CCC(OCCOC(CCC(ON2C(CCC2=O)=O)=O)=O)=O, Molecular C18H20N2O12Journal, Comparative Study, Article, Research Support, Non-U.S. Gov’t, Biochemical and Biophysical Research Communications called The dimerization domain of upstream binding factor contains multiple helical structures, Author is Lai, Yu-Shen; Tseng, Hua-Bin; Hu, Chin-Hwa, the main research direction is transcription factor UBF dimerization binding DNA; upstream binding factor dimerization binding DNA.Formula: C18H20N2O12.

The upstream binding factor, UBF, is an RNA polymerase I transcription factor which contains multiple DNA binding domains and a novel protein dimerization domain. Active UBF forms homodimers in vivo through the intramol. interactions of its dimerization domain, which spans a hundred amino-terminal residues. In the presence of both UBF dimerization domain and its immediately adjacent lysine-rich basic DNA binding domain, the E. coli expressed recombinant polypeptide, dbUBF (dimerization plus basic motifs of UBF), forms homodimers in vitro and binds to double-stranded DNA nonselectively. In gel retardation assay, dbUBF dimers make multiple shift-ladders corresponding to numerous protein dimer-DNA complexes. The UBF dimerization domain contains multiple helical structures, as predicted by EMBO-PHD program. Most of hydrophobic residues in the dimerization domain are confirmed in the hydrophobic phase of these hypothetic helixes. Mutating these hydrophobic residues to glutamate prohibits dbUBF association and gives a different shift pattern in gel retardation assay. The results we present here argue that UBF association is largely exerted by the hydrophobic interactions between the multiple helixes to bring two mols. together.

Different reactions of this compound(Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate)Formula: C18H20N2O12 require different conditions, so the reaction conditions are very important.

Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Awesome and Easy Science Experiments about 70539-42-3

Different reactions of this compound(Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate)Recommanded Product: 70539-42-3 require different conditions, so the reaction conditions are very important.

Recommanded Product: 70539-42-3. Aromatic heterocyclic compounds can also be classified according to the number of heteroatoms contained in the heterocycle: single heteroatom, two heteroatoms, three heteroatoms and four heteroatoms. Compound: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, is researched, Molecular C18H20N2O12, CAS is 70539-42-3, about Chemical crosslinking of mitochondrial NADH dehydrogenase from bovine heart. Author is Cleeter, Michael W. J.; Banister, Susan H.; Ragan, C. Ian.

The structure of bovine heart mitochondrial NADH dehydrogenase was investigated by using 2 cleavable crosslinking agents, disuccinimidyl tartrate and (ethylene glycol)yl bis(succinimidyl succinate). Crosslinking was analyzed primarily by immunoblotting to detect products containing subunits of the Fe-protein fraction from chaotropic resolution of the enzyme, namely those of 75, 49, 30, and 13 kilodaltons. Crosslinks were identified between these 4 subunits, from these subunits to the largest subunit of the flavoprotein fraction (which contains the active site for NADH), and from these subunits to polypeptides in the hydrophobic shell (which surrounds the hydrophilic Fe-protein and flavoprotein fractions).

Different reactions of this compound(Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate)Recommanded Product: 70539-42-3 require different conditions, so the reaction conditions are very important.

Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Never Underestimate the Influence Of 70539-42-3

《Immobilization of perfluoroalkylated enzymes in a biologically active state onto Perflex support》 provides a strategy for the preparation of materials with excellent comprehensive properties, which is conducive to broaden the application field of this compound(Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate)Category: benzofurans.

In organic chemistry, atoms other than carbon and hydrogen are generally referred to as heteroatoms. The most common heteroatoms are nitrogen, oxygen and sulfur. Now I present to you an article called Immobilization of perfluoroalkylated enzymes in a biologically active state onto Perflex support, published in 1991-10-31, which mentions a compound: 70539-42-3, mainly applied to Perflex perfluoroalkylated enzyme immobilization, Category: benzofurans.

Perflex was introduced as a new fluorocarbon-based technol. for protein immobilization. Due to the hydrophobic character of the support, however, significant loss of enzymic activity may occur upon immobilization of certain enzymes, which appears to be due to a large conformational change of the protein (inversion). Pretreatment of the Perflex support with a neutral fluorosurfactant lessened the surface hydrophobicity, thus decreasing the hydrophobic interaction between the support and the protein. Modification of enzymes with a high number of fluorocarbon residues, which forms a hydrophobic envelope around the protein, also appeared to prevent enzyme inactivation upon immobilization on Perflex support. Moreover, preactivation of the support with either perfluorooctylpropylisocyanate or reactive poly(fluoroalkyl)sugar reagents greatly improved the enzyme particle activity by increasing the amount of immobilized enzyme. Fluorosurfactant treatment of the support activated with perfluorooctylpropylisocyanate improved the retention of activity for sensitive enzymes such as chymotrypsin and increased the wet ability and ease of handling of the Perflex particles.

《Immobilization of perfluoroalkylated enzymes in a biologically active state onto Perflex support》 provides a strategy for the preparation of materials with excellent comprehensive properties, which is conducive to broaden the application field of this compound(Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate)Category: benzofurans.

Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Properties and Exciting Facts About 70539-42-3

《Congo red interaction with alpha-proteins.》 provides a strategy for the preparation of materials with excellent comprehensive properties, which is conducive to broaden the application field of this compound(Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate)Formula: C18H20N2O12.

The reaction of an aromatic heterocycle with a proton is called a protonation. One of articles about this theory is 《Congo red interaction with alpha-proteins.》. Authors are Sereikaite, Jolanta; Bumelis, Vladas-Algirdas.The article about the compound:Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinatecas:70539-42-3,SMILESS:O=C(ON1C(CCC1=O)=O)CCC(OCCOC(CCC(ON2C(CCC2=O)=O)=O)=O)=O).Formula: C18H20N2O12. Through the article, more information about this compound (cas:70539-42-3) is conveyed.

The ability of Congo red to form complexes with alpha-proteins, human growth hormone and human interferon-alpha2b, was found by absorption difference spectroscopy. A human growth hormone-Congo red complex was isolated by gel-permeation chromatography, and its visible absorption spectrum was registered in comparison to free dye. The ability of Congo red to induce dimerization of human growth hormone was demonstrated using chemical cross-linking agents 1,3,5-triacryloyl-hexahydro-s-triazine and ethylene glycol bis(succinimidylsuccinate).

《Congo red interaction with alpha-proteins.》 provides a strategy for the preparation of materials with excellent comprehensive properties, which is conducive to broaden the application field of this compound(Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate)Formula: C18H20N2O12.

Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Research on new synthetic routes about 70539-42-3

Different reactions of this compound(Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate)Reference of Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate require different conditions, so the reaction conditions are very important.

Reference of Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate. The protonation of heteroatoms in aromatic heterocycles can be divided into two categories: lone pairs of electrons are in the aromatic ring conjugated system; and lone pairs of electrons do not participate. Compound: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, is researched, Molecular C18H20N2O12, CAS is 70539-42-3, about A mitogenic fibrinogen receptor that differs from glycoprotein IIb-IIIa. Identification by affinity chromatography and by covalent cross-linking. Author is Levesque, Jean Pierre; Hatzfeld, Jacques; Hatzfeld, Antoinette.

The mitogenic effect of human fibrinogen on the hemopoietic cell lines Raji and JM is mediate by a specific receptor with biochem. and functional properties different from those of the platelet fibrinogen receptor, the glycoprotein complex IIb-IIIa. This work describes the identification of the mitogenic fibrinogen receptor (MFR) by 2 independent methods, affinity chromatog. and covalent crosslinking. Affinity chromatog. of surface-labeled cell extracts on fibrinogen-Sepharose revealed a 94-kDa membrane protein that bound specifically to fibrinogen-Sepharose only on cells that expressed the MFR. Its mol. mass was not modified after reduction This was confirmed by crosslinking fibrinogen to surface-labeled Raji cells using the cleavable crosslinkers, ethyleneglycolbis(succinimidyl succinate) and dithiobis(succinimidyl propionate). Complexes between fibrinogen and iodinated cell membrane proteins were immunoprecipitated by anti-fibrinogen antibodies. The biochem. cleavage of these immunoprecipitated conjugates gave rise to a 92-kDa membrane protein whose mol. mass was not modified after reduction Thus, fibrinogen binds specifically to a 92-94-kDa MFR which does not belong to the integrin family.

Different reactions of this compound(Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate)Reference of Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate require different conditions, so the reaction conditions are very important.

Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem