Heterocyclic compounds can be divided into two categories: alicyclic heterocycles and aromatic heterocycles. Compounds whose heterocycles in the molecular skeleton cannot reflect aromaticity are called alicyclic heterocyclic compounds. Compound: 70539-42-3, is researched, Molecular C18H20N2O12, about Identification of a receptor for peptides of the bombesin family in Swiss 3T3 cells by affinity cross-linking, the main research direction is receptor bombesin peptide 3T3 cell; affinity crosslinking bombesin receptor.Recommanded Product: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate.
The cross-linking agent ethylene glycolbis(succinimidyl succinate) was used to covalently link 125I-labeled gastrin-releasing peptide (125I-GRP) [80043-53-4] to a 75,000-85,000-mol.-weight surface protein in Swiss 3T3 cells that displays many characteristics of a specific receptor for peptides of the bombesin family. This protein was not present in other cell lines which do not exhibit receptors for bombesin-like peptides. Unlabeled GRP competed for affinity labeling of the 75,000-85,000-mol.-weight protein in a concentration-dependent manner, and other bombesin-related peptides also inhibited the cross-linking of 125I-GRP to this component. In contrast, high concentrations of a variety of other peptide hormones and mitogens had no effect. Affinity labeling of the 75,000-85,000-mol.-weight protein was dependent on the concentration of 125I-GRP and exhibited saturability. 125I-GRP affinity labeling of this protein was also demonstrated by 2-dimensional gel electrophoresis. These studies suggest that an 75,000-85,000-mol.-weight surface protein with an isoelec. point of 6.0-6.5 is a major component of the receptor for peptides of the bombesin family in Swiss 3T3 cells.
Different reactions of this compound(Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate)Recommanded Product: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate require different conditions, so the reaction conditions are very important.
Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem