Most of the natural products isolated at present are heterocyclic compounds, so heterocyclic compounds occupy an important position in the research of organic chemistry. A compound: 70539-42-3, is researched, SMILESS is O=C(ON1C(CCC1=O)=O)CCC(OCCOC(CCC(ON2C(CCC2=O)=O)=O)=O)=O, Molecular C18H20N2O12Journal, Comparative Study, Article, Research Support, Non-U.S. Gov’t, Biochemical and Biophysical Research Communications called The dimerization domain of upstream binding factor contains multiple helical structures, Author is Lai, Yu-Shen; Tseng, Hua-Bin; Hu, Chin-Hwa, the main research direction is transcription factor UBF dimerization binding DNA; upstream binding factor dimerization binding DNA.Formula: C18H20N2O12.
The upstream binding factor, UBF, is an RNA polymerase I transcription factor which contains multiple DNA binding domains and a novel protein dimerization domain. Active UBF forms homodimers in vivo through the intramol. interactions of its dimerization domain, which spans a hundred amino-terminal residues. In the presence of both UBF dimerization domain and its immediately adjacent lysine-rich basic DNA binding domain, the E. coli expressed recombinant polypeptide, dbUBF (dimerization plus basic motifs of UBF), forms homodimers in vitro and binds to double-stranded DNA nonselectively. In gel retardation assay, dbUBF dimers make multiple shift-ladders corresponding to numerous protein dimer-DNA complexes. The UBF dimerization domain contains multiple helical structures, as predicted by EMBO-PHD program. Most of hydrophobic residues in the dimerization domain are confirmed in the hydrophobic phase of these hypothetic helixes. Mutating these hydrophobic residues to glutamate prohibits dbUBF association and gives a different shift pattern in gel retardation assay. The results we present here argue that UBF association is largely exerted by the hydrophobic interactions between the multiple helixes to bring two mols. together.
Different reactions of this compound(Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate)Formula: C18H20N2O12 require different conditions, so the reaction conditions are very important.
Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem