Reference of Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate. The protonation of heteroatoms in aromatic heterocycles can be divided into two categories: lone pairs of electrons are in the aromatic ring conjugated system; and lone pairs of electrons do not participate. Compound: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, is researched, Molecular C18H20N2O12, CAS is 70539-42-3, about A mitogenic fibrinogen receptor that differs from glycoprotein IIb-IIIa. Identification by affinity chromatography and by covalent cross-linking. Author is Levesque, Jean Pierre; Hatzfeld, Jacques; Hatzfeld, Antoinette.
The mitogenic effect of human fibrinogen on the hemopoietic cell lines Raji and JM is mediate by a specific receptor with biochem. and functional properties different from those of the platelet fibrinogen receptor, the glycoprotein complex IIb-IIIa. This work describes the identification of the mitogenic fibrinogen receptor (MFR) by 2 independent methods, affinity chromatog. and covalent crosslinking. Affinity chromatog. of surface-labeled cell extracts on fibrinogen-Sepharose revealed a 94-kDa membrane protein that bound specifically to fibrinogen-Sepharose only on cells that expressed the MFR. Its mol. mass was not modified after reduction This was confirmed by crosslinking fibrinogen to surface-labeled Raji cells using the cleavable crosslinkers, ethyleneglycolbis(succinimidyl succinate) and dithiobis(succinimidyl propionate). Complexes between fibrinogen and iodinated cell membrane proteins were immunoprecipitated by anti-fibrinogen antibodies. The biochem. cleavage of these immunoprecipitated conjugates gave rise to a 92-kDa membrane protein whose mol. mass was not modified after reduction Thus, fibrinogen binds specifically to a 92-94-kDa MFR which does not belong to the integrin family.
Different reactions of this compound(Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate)Reference of Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate require different conditions, so the reaction conditions are very important.
Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem