Recommanded Product: 70539-42-3. Aromatic heterocyclic compounds can also be classified according to the number of heteroatoms contained in the heterocycle: single heteroatom, two heteroatoms, three heteroatoms and four heteroatoms. Compound: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, is researched, Molecular C18H20N2O12, CAS is 70539-42-3, about Chemical crosslinking of mitochondrial NADH dehydrogenase from bovine heart. Author is Cleeter, Michael W. J.; Banister, Susan H.; Ragan, C. Ian.
The structure of bovine heart mitochondrial NADH dehydrogenase was investigated by using 2 cleavable crosslinking agents, disuccinimidyl tartrate and (ethylene glycol)yl bis(succinimidyl succinate). Crosslinking was analyzed primarily by immunoblotting to detect products containing subunits of the Fe-protein fraction from chaotropic resolution of the enzyme, namely those of 75, 49, 30, and 13 kilodaltons. Crosslinks were identified between these 4 subunits, from these subunits to the largest subunit of the flavoprotein fraction (which contains the active site for NADH), and from these subunits to polypeptides in the hydrophobic shell (which surrounds the hydrophilic Fe-protein and flavoprotein fractions).
Different reactions of this compound(Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate)Recommanded Product: 70539-42-3 require different conditions, so the reaction conditions are very important.
Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem