The reaction of an aromatic heterocycle with a proton is called a protonation. One of articles about this theory is 《Sequence of release of neurohormones on nervous stimulation of frog’s stomach muscle》. Authors are Singh, I..The article about the compound:Sodium 4-butyl-3,5-dioxo-1,2-diphenylpyrazolidin-4-idecas:129-18-0,SMILESS:O=C(N(C1=CC=CC=C1)N2C3=CC=CC=C3)[C-](CCCC)C2=O.[Na+]).COA of Formula: C19H19N2NaO2. Through the article, more information about this compound (cas:129-18-0) is conveyed.
The experiments were performed on the stomach muscle of Rana tigrina. The nerve-muscle preparations were made as described by S., et al. (CA 56, 9285e), and were immersed in saline, or in 0.112M sucrose. The contraction produced by nerve stimulation showed seasonal variations. In summer (room temperature 29-31°), the latent period of response was 2-5 sec., and in winter (room temperature 23-5°), the latent period was 20-50 sec. In winter and in summer, atropine sulfate (I) did not abolish the contraction, unless it was previously treated with 2-bromo-D-lysergic acid diethylamide (II). Neopyramine (III) did not abolish the contraction of untreated muscles, but it abolished the contraction of muscles treated with I and II. The effects of II are different in summer and in winter. In winter, the contraction were diminished or abolished. In summer, the contractions were enhanced, but if the muscles were soaked in sucrose solution, the effect in summer was similar to that in winter. A combination of I, II, and III did not affect, or slightly diminished contractions in summer and in winter. If the muscle is soaked in sucrose, the contraction persists, indicating a release of substance P. Substance P itself caused contraction in sucrose-soaked muscles. As in winter, nervous stimulation in summer also produced relaxation, which was abolished by II the relaxation produced by adrenaline or noradrenaline was not affected.
Here is a brief introduction to this compound(129-18-0)COA of Formula: C19H19N2NaO2, if you want to know about other compounds related to this compound(129-18-0), you can read my other articles.
Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem