In organic chemistry, atoms other than carbon and hydrogen are generally referred to as heteroatoms. The most common heteroatoms are nitrogen, oxygen and sulfur. Now I present to you an article called Covalent labeling of vasopressin receptors from LLC-PK1 cells by the use of a bifunctional reagent, published in 1988-04-30, which mentions a compound: 70539-42-3, mainly applied to ethyleneglycolsuccinimidyl succinate vasopressin receptor complexation, Category: benzofurans.
The possibility of covalently attaching vasopressin to its receptors by the use of a bifunctional reagent was explored. Plasma membranes from the LLC-PK1 pig kidney cell line were purified by Percoll d. gradient centrifugation. These membranes contained a single population of high affinity (dissociation constant = 5.2 nM) and high maximum binding capacity (3.8 pmol/mg protein) [3H]lysine vasopressin ([3H]LVP)-binding sites. [3H]LVP-labeled receptors were solubilized with a high yield (83%) and minimal dissociation (9%) by treatment with the nonionic detergent, octaethylene glycol monododecyl ether (0.5%) in the presence of glycerol (20%). The solubilized [3H]LVP-labeled receptors were stable upon storage at 4° (5% dissociation after 24 h). They were partially purified to a specific activity of 17 pmol/mg of protein by chromatog. on a Cibacron blue-Sepharose column with a yield of 90%. The [3H]LVP-receptor complexes in both intact membranes and the partially purified preparation were almost completely dissociated by incubation at 30° for 30 min in the presence of 20 mM EDTA. This property was used to test the effect of ethylene glycol bis(succinimidyl-succinate) (EGS) as crosslinking reagent for the covalent attachment of [3H]LVP to its receptors. After treatment of [3H]LVP-labeled membranes for 30 min with 1 mM EGS at 4°, about 30% of specifically bound [3H]LVP was resistant to EDTA dissociation The amount of EDTA-resistant binding varied as a linear function of the fractional receptor occupancy and maximal binding capacity of the different batches of membranes used. Similar results were obtained with solubilized and partially purified vasopressin receptors. Upon steric exclusion HPLC, the EDTA-resistant [3H]LVP-labeled material, like the native [3H]LVP-labeled receptor, was eluted as a single and apparently homogeneous peak. The covalent character of the EGS-induced [3H]LVP binding to solubilized or partially purified receptors was assessed by its resistance to SDS-PAGE. The yield of EGS-induced labeling deduced from these experiments (27%) was close to that determined by the EDTA method. SDS-PAGE anal. of the [3H]LVP-labeled cross-linked material revealed the specific labeling of a major 50 kilodalton (kDa) component and a minor component of 30 kDa. The size of these 2 components was not affected by dithiothreitol.
Here is a brief introduction to this compound(70539-42-3)Category: benzofurans, if you want to know about other compounds related to this compound(70539-42-3), you can read my other articles.
Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem