Category: 90866-33-4

Shimizu, Sakayu; Kataoka, Michihiko; Kita, Keiko published the article 《Chiral alcohol synthesis with microbial carbonyl reductases in a water-organic solvent two-phase system》. Keywords: asym microbial reduction chloroacetoacetate; chlorohydroxybutanoate asym microbial preparation; aldehyde reductase asym reduction chloroacetoacetate; Sporobolomyces salmonicolor enzyme asym reduction chloroacetoacetate; Candida magnoliae enzyme asym reduction chloroacetoacetate.They researched the compound: (R)-Ethyl 4-chloro-3-hydroxybutanoate( cas:90866-33-4 ).SDS of cas: 90866-33-4. Aromatic heterocyclic compounds can be divided into two categories: single heterocyclic and fused heterocyclic. In addition, there is a lot of other information about this compound (cas:90866-33-4) here.

Production of chiral Et 4-chloro-3-hydroxybutanoate (CHBE) was performed through microbial asym. reduction of Et 4-chloroacetoacetate (CAAE). The enzymes reducing CAAE to (R)- and (S)-CHBE were found to be produced by Sporobolomyces salmonicolor and Candida magnoliae, resp. The enzyme of S. salmonicolor is a novel NADPH-dependent aldehyde reductase (AR) belonging to the aldo-keto reductase superfamily. When AR-overproducing Escherichia coli transformant cells or C. magnoliae cells were incubated in an organic solvent-water two-phase system, 300 or 90 mg/mL of CAAE was almost stoichiometrically converted to (R)- or (S)-CHBE (>92% ee), resp.

There is still a lot of research devoted to this compound(SMILES：O=C(OCC)C[C@@H](O)CCl)SDS of cas: 90866-33-4, and with the development of science, more effects of this compound(90866-33-4) can be discovered.

Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Quality Control of (R)-Ethyl 4-chloro-3-hydroxybutanoate. The reaction of aromatic heterocyclic molecules with protons is called protonation. Aromatic heterocycles are more basic than benzene due to the participation of heteroatoms. Compound: (R)-Ethyl 4-chloro-3-hydroxybutanoate, is researched, Molecular C6H11ClO3, CAS is 90866-33-4, about Synthesis of ethyl (R)-4-chloro-3-hydroxybutyrate by immobilized cells using amino acid-modified magnetic nanoparticles. Author is Lu, Yuan; Dai, Hongqian; Shi, Hanbing; Tang, Lan; Sun, Xingyuan; Ou, Zhimin.

Fe3O4-Arg was selected as the optimal carrier due to its high activity recovery of immobilized cells in the preparation of Fe3O4-Arg-Cells. The optimal immobilization conditions for the preparation of Fe3O4-Arg-Cells were 30°C, 4 h, pH 7, and 3 g dry yeast. The activity recovery of immobilized cells reached 76.8%. For a batch reduction in a shaker in an alternating magnetic field, Fe3O4-Arg-Cells were used as a catalyst to gain Et (R)-4-chloro-3-hydroxybutyrate ((R)-CHBE). For further improvement in reduction productivity, a continuous reduction in the magnetic fluidized bed reactor system (MFBRS) was completed. Under their optimal transformation conditions, it took 24 h for Fe3O4-Arg-Cells to complete the conversion of Et 4-chloro-3-oxobutanoate (COBE) (0.8553 mol/L) in the shaker and only 8 h for the batch reduction in an alternating magnetic field. Continuous reduction in MFBRS provided new ideas for the efficient production of (R)-CHBE; 1.5882 mol/L (10 mL) of COBE can be completely converted in 6 h. The conversion and enantiomeric excess (e.e.) of (R)-CHBE were 100% and above 99.9% resp., in the three reaction systems mentioned above.

Here is a brief introduction to this compound(90866-33-4)Quality Control of (R)-Ethyl 4-chloro-3-hydroxybutanoate, if you want to know about other compounds related to this compound(90866-33-4), you can read my other articles.

Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Application of 90866-33-4. So far, in addition to halogen atoms, other non-metallic atoms can become part of the aromatic heterocycle, and the target ring system is still aromatic. Compound: (R)-Ethyl 4-chloro-3-hydroxybutanoate, is researched, Molecular C6H11ClO3, CAS is 90866-33-4, about Stereoselective reduction of ethyl 4-chloro-3-oxobutanoate by Escherichia coli transformant cells coexpressing the aldehyde reductase and glucose dehydrogenase genes.

The asym. reduction of Et 4-chloro-3-oxobutanoate to Et (R)-4-chloro-3-hydroxybutanoate (I) using E. coli cells, which coexpress both the aldehyde reductase gene from Sporobolomyces salmonicolor and the glucose dehydrogenase (GDH) gene from Bacillus megaterium as a catalyst was investigated. In an organic solvent-water 2-phase system, I formed in the organic phase amounted to 1610 mM (268 mg/mL), with a molar yield of 94.1% and an optical purity of 91.7% e.e. The calculated turnover number of NADP+ to I formed was 13,500 mol/mol. Since the use of E. coli JM109 cells harboring pKAR and pACGD as a catalyst is simple and does not require the addition of GDH or the isolation of the enzymes, it is highly advantageous for the practical synthesis of I.

Here is a brief introduction to this compound(90866-33-4)Application of 90866-33-4, if you want to know about other compounds related to this compound(90866-33-4), you can read my other articles.

Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

The three-dimensional configuration of the ester heterocycle is basically the same as that of the carbocycle. Compound: (R)-Ethyl 4-chloro-3-hydroxybutanoate(SMILESS: O=C(OCC)C[C@@H](O)CCl,cas:90866-33-4) is researched.Recommanded Product: Ethyl 2-(2-oxopyrrolidin-1-yl)acetate. The article 《Efficient synthesis of optically pure alcohols by asymmetric hydrogen-transfer biocatalysis: application of engineered enzymes in a 2-propanol-water medium》 in relation to this compound, is published in Applied Microbiology and Biotechnology. Let’s take a look at the latest research on this compound (cas:90866-33-4).

An efficient method is described for producing both enantiomers of chiral alcs. by asym. hydrogen-transfer bioreduction of ketones in a 2-propanol (IPA)-water medium with Escherichia coli biocatalysts expressing phenylacetaldehyde reductase (PAR: wild-type and mutant enzymes) from Rhodococcus sp. ST-10 and alc. dehydrogenase from Leifsonia sp. S749 (LSADH). Also described are the detailed properties of mutant PARs, Sar268, and HAR1, which were engineered to have high activity and productivity in media composed of polar organic solvent and water, and the construction of three-dimensional structure of PAR by homol. modeling. The Km and Vmax values for some substrates and the substrate specificity of mutant PARs were quite different from those of wild-type PAR. The results well explained the increased productivity of engineered PARs in IPA-water medium.

If you want to learn more about this compound((R)-Ethyl 4-chloro-3-hydroxybutanoate)HPLC of Formula: 90866-33-4, you may wish to communicate with the author of the article,or consult the relevant literature related to this compound(90866-33-4).

Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

The three-dimensional configuration of the ester heterocycle is basically the same as that of the carbocycle. Compound: (R)-Ethyl 4-chloro-3-hydroxybutanoate(SMILESS: O=C(OCC)C[C@@H](O)CCl,cas:90866-33-4) is researched.Safety of Methanesulfonato(2-dicyclohexylphosphino-2′,6′-di-i-propoxy-1,1′-biphenyl)(2′-amino-1,1′-biphenyl-2-yl)palladium(II). The article 《A method for determining ketoreductase activity in broth》 in relation to this compound, is published in Zhongguo Niangzao. Let’s take a look at the latest research on this compound (cas:90866-33-4).

The UV-Vis spectrophotometer method to determine the enzyme activity in fermentation broth was studied using Et 4-chloroacetoacetate (COBE) as substrate, NAD hydro-phosphate acid (NADPH) as a coenzyme. The enzyme activity was calculated by continuously measuring the consumption of NADPH. The optimum reaction conditions were acquired as follows: NADPH concentration 0.2 mmol/L, COBE 1.0 mmol/L, phosphate buffer 100 mmol/L, pH 6 and temperature 40 °C. Finally, five times parallel detections were done to determine the enzyme activity under the optimum reaction system. It showed that relative standard deviation was 0.48%. This method with advantages of simple, short time-consuming, high accuracy, good repeatability could be generally used in the measurement of ketoreductase activity.

If you want to learn more about this compound((R)-Ethyl 4-chloro-3-hydroxybutanoate)Safety of (R)-Ethyl 4-chloro-3-hydroxybutanoate, you may wish to communicate with the author of the article,or consult the relevant literature related to this compound(90866-33-4).

Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Application In Synthesis of (R)-Ethyl 4-chloro-3-hydroxybutanoate. So far, in addition to halogen atoms, other non-metallic atoms can become part of the aromatic heterocycle, and the target ring system is still aromatic. Compound: (R)-Ethyl 4-chloro-3-hydroxybutanoate, is researched, Molecular C6H11ClO3, CAS is 90866-33-4, about Separation of ethyl (R) 4-chloro-3-hydroxybutyrate from racemic mixture by using bulk liquid membrane.

The separation of optically active Et (R) 4-chloro-3-hydroxybutyrate (CHBE), which is precursor of L-carnitine, from its racemic mixture was studied. Bulk liquid membrane was used as a separation method. Racemic mixture was prepared by mixing each pure enantiomers. Carrier material that facilitates transport of CHBE was dissolved in membrane phase and cinchonidine, cyclodextrin were used as carriers. Carrier concentration, pH gradient, salt addition varied during experiment as exptl. parameters. CHBE transported from feed phase to receiving through the membrane phase, but the enantioselectivity was still in low range. Through whole experiment, proper selection of membrane phase and carrier was key factors that affected CHBE separation

If you want to learn more about this compound((R)-Ethyl 4-chloro-3-hydroxybutanoate)Application In Synthesis of (R)-Ethyl 4-chloro-3-hydroxybutanoate, you may wish to communicate with the author of the article,or consult the relevant literature related to this compound(90866-33-4).

Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Computed Properties of C6H11ClO3. The mechanism of aromatic electrophilic substitution of aromatic heterocycles is consistent with that of benzene. Compound: (R)-Ethyl 4-chloro-3-hydroxybutanoate, is researched, Molecular C6H11ClO3, CAS is 90866-33-4, about Stereochemical control in microbial reduction. Part 13. A novel method to synthesize (L)-β-hydroxyl esters by the reduction with bakers’ yeast. Author is Nakamura, Kaoru; Kawai, Yasushi; Ohno, Atsuyoshi.

RCH2COCH2CO2R1 (R = R1 = Me; R = H, Cl, Me, CF3, R1 = Et) were reduced stereoselectively to (L)-RCH2CH(OH)CH2CO2R1 (enantiomeric excess of 80-94%) with baker’s yeast in the presence of ClCH2CO2Et (I). I works by inhibiting D-hydrogenases in the complex mixture of enzymes found in baker’s yeast. Other halo ester additives were investigated.

There is still a lot of research devoted to this compound(SMILES：O=C(OCC)C[C@@H](O)CCl)Computed Properties of C6H11ClO3, and with the development of science, more effects of this compound(90866-33-4) can be discovered.

Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

The chemical properties of alicyclic heterocycles are similar to those of the corresponding chain compounds. Compound: (R)-Ethyl 4-chloro-3-hydroxybutanoate, is researched, Molecular C6H11ClO3, CAS is 90866-33-4, about Production of ethyl 4-chloro-3-hydroxybutanoate with resting cells of Sulfolobus solfataricus, the main research direction is chloroacetoacetate asym reduction Sulfolobus; chiral chlorohydroxybutanoate production Sulfolobus.Safety of (R)-Ethyl 4-chloro-3-hydroxybutanoate.

The thermophilic archaebacterium S. solfataricus converted Et 4-chloroacetoacetate to Et (R)-4-chloro-3-hydroxybutanoate in 97% yield, with 98% enantiomeric excess. Ten g wet cells were suspended in 100 mL pH 5 acetate buffer containing 5 mL propan-2-ol and 1 g (60 mM) substrate. After 24 h at 30°, the supernatant was extracted with ether and the product purified by HPLC. Concentrations of substrate >60 mM led to decreased yields.

There is still a lot of research devoted to this compound(SMILES：O=C(OCC)C[C@@H](O)CCl)Safety of (R)-Ethyl 4-chloro-3-hydroxybutanoate, and with the development of science, more effects of this compound(90866-33-4) can be discovered.

Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Epoxy compounds usually have stronger nucleophilic ability, because the alkyl group on the oxygen atom makes the bond angle smaller, which makes the lone pair of electrons react more dissimilarly with the electron-deficient system. Compound: (R)-Ethyl 4-chloro-3-hydroxybutanoate, is researched, Molecular C6H11ClO3, CAS is 90866-33-4, about Asymmetric reduction of ethyl 4-chloroacetoacetate to ethyl (R)-(+)-4-chloro-3-hydroxybutyrate by recombinant Escherichia coli.Electric Literature of C6H11ClO3.

The NADPH-dependent aldehyde reductase gene was cloned from the genome of Sporobolomyces salmonicolor ZJU0105, and the recombinant E. coli BL21 (pET28-ALR0105) strain was constructed. The asym. reduction of Et 4-chloroacetoacetate (COBE) to Et (R)-(+)-4-chloro-3-hydroxybutyrate ((R)-CHBE) by the recombinant cells in an aqueous phase was investigated. The product is optically pure (R)-CHBE, and both the (R)-CHBE yield and stereoselectivity are higher than those in the reaction catalyzed by Sporobolomyces salmonicolor. The glucose dehydrogenase, NADPH and glucose are required for the recombinant cells to regenerate NADPH. The reaction is also inhibited by high concentration of COBE and CHBE, but high d. of recombinant cells can decrease this inhibitory effect. A yield of 96.5% and ee of 99% for (R)-CHBE are obtained under the appropriate reaction conditions.

If you want to learn more about this compound((R)-Ethyl 4-chloro-3-hydroxybutanoate)Electric Literature of C6H11ClO3, you may wish to communicate with the author of the article,or consult the relevant literature related to this compound(90866-33-4).

Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Formula: C6H11ClO3. The fused heterocycle is formed by combining a benzene ring with a single heterocycle, or two or more single heterocycles. Compound: (R)-Ethyl 4-chloro-3-hydroxybutanoate, is researched, Molecular C6H11ClO3, CAS is 90866-33-4, about Asymmetric synthesis of ethyl 4-chloro-(R)-3-hydroxybutyrate with baker’s yeast. Author is Yi, Ming-jun; Song, Guang-liang; Zhu, Hong-jun; Wang, Jin-tang.

Optically active Et 4-chloro-(R)-3-hydroxybutyrate was prepared by stereoselective bioreduction of Et 4-chloro-acetoacetate with baker’s yeast. Its structure was elucidated by IR,GC-MS and 1HNMR.The factors influencing the synthesis were investigated. The results showed that the optimal reaction condition as follows: baker’s yeast 600 g/L, mass concentration of glucose 20 g/L, feeding rate of substrate 16 mL/L,pH 5, reaction time 48 h, temperature 34 °C. The sp. rotation of the product was [α]20D = +13.9°.

If you want to learn more about this compound((R)-Ethyl 4-chloro-3-hydroxybutanoate)Formula: C6H11ClO3, you may wish to communicate with the author of the article,or consult the relevant literature related to this compound(90866-33-4).

Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem