The three-dimensional configuration of the ester heterocycle is basically the same as that of the carbocycle. Compound: (R)-Ethyl 4-chloro-3-hydroxybutanoate(SMILESS: O=C(OCC)C[C@@H](O)CCl,cas:90866-33-4) is researched.Safety of Methanesulfonato(2-dicyclohexylphosphino-2′,6′-di-i-propoxy-1,1′-biphenyl)(2′-amino-1,1′-biphenyl-2-yl)palladium(II). The article 《A method for determining ketoreductase activity in broth》 in relation to this compound, is published in Zhongguo Niangzao. Let’s take a look at the latest research on this compound (cas:90866-33-4).
The UV-Vis spectrophotometer method to determine the enzyme activity in fermentation broth was studied using Et 4-chloroacetoacetate (COBE) as substrate, NAD hydro-phosphate acid (NADPH) as a coenzyme. The enzyme activity was calculated by continuously measuring the consumption of NADPH. The optimum reaction conditions were acquired as follows: NADPH concentration 0.2 mmol/L, COBE 1.0 mmol/L, phosphate buffer 100 mmol/L, pH 6 and temperature 40 °C. Finally, five times parallel detections were done to determine the enzyme activity under the optimum reaction system. It showed that relative standard deviation was 0.48%. This method with advantages of simple, short time-consuming, high accuracy, good repeatability could be generally used in the measurement of ketoreductase activity.
If you want to learn more about this compound((R)-Ethyl 4-chloro-3-hydroxybutanoate)Safety of (R)-Ethyl 4-chloro-3-hydroxybutanoate, you may wish to communicate with the author of the article,or consult the relevant literature related to this compound(90866-33-4).
Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem