Epoxy compounds usually have stronger nucleophilic ability, because the alkyl group on the oxygen atom makes the bond angle smaller, which makes the lone pair of electrons react more dissimilarly with the electron-deficient system. Compound: (R)-Ethyl 4-chloro-3-hydroxybutanoate, is researched, Molecular C6H11ClO3, CAS is 90866-33-4, about Asymmetric reduction of ethyl 4-chloroacetoacetate to ethyl (R)-(+)-4-chloro-3-hydroxybutyrate by recombinant Escherichia coli.Electric Literature of C6H11ClO3.
The NADPH-dependent aldehyde reductase gene was cloned from the genome of Sporobolomyces salmonicolor ZJU0105, and the recombinant E. coli BL21 (pET28-ALR0105) strain was constructed. The asym. reduction of Et 4-chloroacetoacetate (COBE) to Et (R)-(+)-4-chloro-3-hydroxybutyrate ((R)-CHBE) by the recombinant cells in an aqueous phase was investigated. The product is optically pure (R)-CHBE, and both the (R)-CHBE yield and stereoselectivity are higher than those in the reaction catalyzed by Sporobolomyces salmonicolor. The glucose dehydrogenase, NADPH and glucose are required for the recombinant cells to regenerate NADPH. The reaction is also inhibited by high concentration of COBE and CHBE, but high d. of recombinant cells can decrease this inhibitory effect. A yield of 96.5% and ee of 99% for (R)-CHBE are obtained under the appropriate reaction conditions.
If you want to learn more about this compound((R)-Ethyl 4-chloro-3-hydroxybutanoate)Electric Literature of C6H11ClO3, you may wish to communicate with the author of the article,or consult the relevant literature related to this compound(90866-33-4).
Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem