Day: January 5, 2023

A feasible fluorimetric approach anchored in diaryl pyrrolone derivative for the facile analysis of milnacipran in tablets; evaluation of the method greenness was written by Derayea, Sayed M.;Madian, Hoda;Samir, Ebtihal;Hamad, Ahmed A.;Badr El-Din, Khaled M.. And the article was included in Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy in 2022.Recommanded Product: 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione This article mentions the following:

In the present work a new, feasible, and green approach was employed for the anal. of milnacipran. A drug is used in the management of depression in addition to fibromyalgia. It inhibits of the reuptake of two essential neurotransmitters serotonin and nor-adrenaline. In slightly alk. buffer (pH 8.5) the primary amino group of milnacipran reacted with fluorescamine to give a substituted pyrrolone derivative which exhibited high fluorescence activity. The fluorescence of produced derivative was measured at (λ_ex 385 nm, λ_em 477 nm), and the exptl. factors were cautiously optimized. The measured intensity of fluorescence was plotted vs. the resp. concentration of milnacipran to setup the calibration plot which has a linear concentration range of 50-300 ng/mL. The ICH guidelines were utilized to totally validate the presented approach. In addition the method could be efficiently incorporated in the anal. of com. milnacipram tablets with no considerable effect on the results of the assay for milnacipran. The developed approach is characterized by its high simplicity and greenness of the procedure which make it suitable for routine anal. In the experiment, the researchers used many compounds, for example, 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9Recommanded Product: 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione).

4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9) belongs to benzofurans derivatives. Benzofurans are only weakly aromatic in nature and they are cleaved by many oxidative and reductive conditions. Substituted benzofurans find applications such as fluorescent sensors, oxidants, in drug discovery, and in another field of chemistry and agriculture.Recommanded Product: 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

PLGA protein nanocarriers with tailor-made fluorescence/MRI/PET imaging modalities was written by Zhang, Yajie;Garcia-Gabilondo, Miguel;Grayston, Alba;Feiner, Irene V. J.;Anton-Sales, Irene;Loiola, Rodrigo A.;Llop, Jordi;Ramos-Cabrer, Pedro;Barba, Ignasi;Garcia-Dorado, David;Gosselet, Fabien;Rosell, Anna;Roig, Anna. And the article was included in Nanoscale in 2020.Application of 38183-12-9 This article mentions the following:

Designing theranostic nanocarriers with high protein payload and multimodality tracking without cross interferences between the different imaging probes and the delicate protein cargo is challenging. Here, chem. modifications of poly(lactic-co-glycolic acid) (PLGA) to produce nanocapsules (NCs) that incorporate several imaging moieties are reported. The biocompatible and biodegradable PLGA-NCs can be endowed with a magnetic resonance imaging (MRI) reporter, two fluorescence imaging probes (blue/NIR) and a positron emission tomog. (PET) reporter. The modular integration of these imaging moieties into the shell of the NCs is successfully achieved without affecting the morphochem. properties of the nanocarrier or the protein loading capacity. In vivo biodistribution of the NCs is monitored by MRI, PET and NIRF and the results from different techniques are analyzed comparatively. The viabilities of two different human endothelial cells in vitro show no toxicity for NC concentration up to 100μg mL^-1. The morbidity of mice for 2 wk after systemic administration and the hepatic/pancreatic enzymes at the plasma level indicate their in vivo biosafety. In summary, the new theranostic PLGA nanoplatform presented here shows versatile in vitro/in vivo multimodal imaging capabilities, excellent biosafety and over 1 wt% protein loading. In the experiment, the researchers used many compounds, for example, 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9Application of 38183-12-9).

4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9) belongs to benzofurans derivatives. Benzofurans are only weakly aromatic in nature and they are cleaved by many oxidative and reductive conditions. In nature, benzofurans have occupied an important role among the plant phenols & several pharmacologically active compounds.Application of 38183-12-9

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

A novel spectrofluorimetric method for the determination of calcitonin in ampuls through derivatization with fluorescamine was written by Kocdan, Deniz;Basan, Hasan. And the article was included in Luminescence in 2013.Product Details of 38183-12-9 This article mentions the following:

In this study, a simple, sensitive, and selective spectrofluorimetric method was developed for the determination of salmon calcitonin (sCT) in ampuls. The method is based on the reaction between sCT and fluorescamine at pH 8.5 in borate buffer, resulting in a highly fluorescent derivative Fluorescence of derivatized sCT solutions was measured by setting the excitation and emission monochromators and slit widths to 390, 484, and 10 nm, resp. Several derivatization parameters were optimized. A calibration graph was constructed using standard solutions of the derivatized calcitonin in the range 0.5-6.0 μg/mL. Limit of detection and limit of quantification values were determined to be 0.124 and 0.372 μg/mL, resp. The proposed method was successfully applied for the determination of sCT in com. available ampuls. High recovery values (101.0-102.0%), and a low relative standard deviation (RSD %) value (5.3-5.4) proved the accuracy and precision of the proposed method. An isocratic reversed-phase high-performance liquid chromatog. (HPLC) method, as a reference, was also developed for the determination of sCT. A reversed-phase Nucleosil C₁₈ column (250 mm × 4.6 mm i.d., 10 μm particle size, 120 Å pore size) was used and the detector was set at 210 nm. Statistical comparison of the results of the 2 methods showed clearly that there was no significant difference between them. Copyright © 2012 John Wiley & Sons, Ltd. In the experiment, the researchers used many compounds, for example, 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9Product Details of 38183-12-9).

4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9) belongs to benzofurans derivatives. Benzofuran is a core structural unit found in many naturally occurring compounds with multidirectional biological activities. Benzofurans are stable towards alkali and readily polymerize on treatment with sulfuric acid, due to which they are useful for the preparation of low cost chemically relatively inert resins.Product Details of 38183-12-9

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

P2X7 receptor deletion attenuates oxidative stress and liver damage in sepsis was written by Larrouyet-Sarto, Maria Luciana;Tamura, Augusto Shuiti;Alves, Vinicius Santos;Santana, Patricia T.;Ciarlini-Magalhaes, Roberta;Rangel, Thuany Prado;Siebert, Cassiana;Hartwig, Josiane R.;Marcon dos Santos, Tiago;Wyse, Angela T. S.;Takiya, Christina Maeda;Coutinho-Silva, Robson;Savio, Luiz Eduardo Baggio. And the article was included in Purinergic Signalling in 2020.Recommanded Product: 76-54-0 This article mentions the following:

Sepsis is a severe disease characterized by an uncontrolled systemic inflammation and consequent organ dysfunction generated in response to an infection. Extracellular ATP acting through the P2X7 receptor induces the maturation and release of pro-inflammatory cytokines (i.e., IL-1β) and the production of reactive nitrogen and oxygen species that lead to oxidative tissue damage. Here, we investigated the role of the P2X7 receptor in inflammation, oxidative stress, and liver injury in sepsis. Sepsis was induced by cecal ligation and puncture (CLP) in wild-type (WT) and P2X7 knockout (P2X7-/-) mice. The oxidative stress in the liver of septic mice was assessed by 2′,7′-dichlorofluorescein oxidation reaction (DCF), thiobarbituric acid-reactive substances (TBARS), and nitrite levels dosage. The status of the endogenous defense system was evaluated through catalase (CAT) and superoxide dismutase (SOD) activities. The inflammation was assessed histol. and by determining the expression of inflammatory cytokines and chemokines by RT-qPCR. We observed an increase in the reactive species and lipid peroxidation in the liver of septic WT mice, but not in the liver from P2X7-/- animals. We found an imbalance SOD/CAT ratio, also only WT septic animals. The number of inflammatory cells and the gene expression of IL-1 β, IL-6, TNF-α, IL-10, CXCL1, and CXCL2 were higher in the liver of WT septic mice in comparison to P2X7-/- septic animals. In summary, our results suggest that the P2X7 receptor might be a therapeutic target to limit oxidative stress damage and liver injury during sepsis. In the experiment, the researchers used many compounds, for example, 2′,7′-Dichloro-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 76-54-0Recommanded Product: 76-54-0).

2′,7′-Dichloro-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 76-54-0) belongs to benzofurans derivatives. Benzofurans are compounds with a planar structure having 10 pi electrons that include the lone pair on oxygen atom, which makes it more susceptible to electrophilic attack. In nature, benzofurans have occupied an important role among the plant phenols & several pharmacologically active compounds.Recommanded Product: 76-54-0

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Development of a novel and synthetic HematoMiR technology that broadly modulates quiescence of stem cells and enhances HSC expansion was written by Uslu, Merve;Kocabas, Fatih. And the article was included in Cellular and Molecular Life Sciences in 2022.Name: 2′,7′-Dichloro-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one This article mentions the following:

Hematopoietic stem cell (HSCs) transplantation is the primary therapeutic modality used to treat hematopoietic disorders. It centers on the capability of a small quantity of HSCs to repopulate whole blood lineages. Along with limited availability of suitable donors, the need for sufficient number of donor HSCs is still challenging in clin. relevance. This has been addressed by ex vivo HSC expansion albeit with partial success, and thus development of an alternative strategy that could improve HSC expansion is required. To that end, we aimed to build HematoMiR, an oligo-based technol. that broadly targets HSC quiescence factors. Here, we show that HematoMiRs and their combinations targeting over 50 factors involved in HSC quiescence could induce robust ex vivo murine and human HSC expansion. In particular, HematoMiR-5 treatment enhanced cell cycle through down-regulation of neg. cell cycle regulators in HSCs. HematoMiR-5 treated HSPCs had reduced DNA damage during the course of ex vivo expansion. Moreover, HematoMiR-5 treatment led to sustained HSC self-renewal ability and a low apoptosis rate. In addition, HematoMiR-5 expanded HSCs demonstrated successful engraftment and repopulation capacity in the recipient animals. Furthermore, combinatorial treatments of HematoMiR-2 and 5 allowed vigorous ex vivo HSC expansion. These findings demonstrate that novel and synthetic HematoMiR technol. is feasible for HSC ex vivo expansion through the sequence-dependent modulation of numerous HSC quiescence modulators. In the experiment, the researchers used many compounds, for example, 2′,7′-Dichloro-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 76-54-0Name: 2′,7′-Dichloro-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one).

2′,7′-Dichloro-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 76-54-0) belongs to benzofurans derivatives. Benzofurans are compounds with a planar structure having 10 pi electrons that include the lone pair on oxygen atom, which makes it more susceptible to electrophilic attack. As benzofurans are prone to undergo ring opening of the heterocycle, examples of reduction of this type of aromatics by using dissolving metals are rather scarce.Name: 2′,7′-Dichloro-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

A new model applied for evaluating a rhenium-diselenium drug: breast cancer cells stimulated by cytokines induced from polynuclear cells by LPS was written by Veena, Vijay;Harikrishnan, Adhikesavan;Lakshmi, Basavegowda;Khanna, Sunali;Desmaele, Didier;Collery, Philippe. And the article was included in Anticancer Research in 2020.Recommanded Product: 76-54-0 This article mentions the following:

Background/aim: New anticancer drugs are usually tested on cancer cells in culture in a standard medium. We stimulated immune polynuclear cells by lipopolysaccharides to obtain an enriched medium (EM) containing inflammatory cytokines more closely reflecting the tumor microenvironment and tested a rhenium-diselenium (Re-diSe) drug in this new model. Concentrations of cytokines were compared with a control medium (CM).</div> Materials and methods: Human-derived breast cancer cells were grown in culture either in CM or EM with or without Re-diSe. Assays of tumor necrosis factor alpha (TNFα), interleukin 6 (IL6), interleukin 1 beta (IL1β), transforming growth factor-beta (TGFβ), insulin growth factor 1 (IGF1) and vascular epidermal growth factor A (VEGFA) were performed by enzyme-linked immunosorbent assays. The production of reactive oxygen species (ROS) was determined by 2,7-dichlorofluorescein test. The cell growth was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide tests. Results: Concentrations of TNFα, IL6 and Il1β were observed to be significantly higher in EM than in CM. There was no difference for TGFβ, IGF1 and VEGFA. The cells were sensitive to Re-diSe, with reduced concentrations of TGFβ, IGF1, VEGFA and ROS, but the half-maximal inhibitory concentration was significantly higher in EM than in CM. Conclusion: The efficacy of the Re-diSe drug was confirmed in this model of aggressive cancer. In the experiment, the researchers used many compounds, for example, 2′,7′-Dichloro-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 76-54-0Recommanded Product: 76-54-0).

2′,7′-Dichloro-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 76-54-0) belongs to benzofurans derivatives. Benzofurans are compounds with a planar structure having 10 pi electrons that include the lone pair on oxygen atom, which makes it more susceptible to electrophilic attack. Introduction of benzofurans in organic synthesis, particularly drug synthesis, involves generally the use of their metalated species as nucleophiles in addition reactions or in metal-catalysed cross-coupling reactions.Recommanded Product: 76-54-0

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Conjugates of Immunoglobulin-Binding Protein and Gold Nanoparticle: Determination of Composition and Application in Immunochromatographic Analysis of Sulfonylamide was written by Sotnikov, D. V.;Barshevskaya, L. V.;Zherdev, A. V.;Dzantiev, B. B.. And the article was included in Applied Biochemistry and Microbiology in 2022.COA of Formula: C17H10O4 This article mentions the following:

A new method for determining the composition of protein-nanoparticle conjugates is proposed in this paper that uses fluorescamine as a fluorescent marker. This method allows obtaining high accuracy results and is characterized by minimal duration and laboriousness. Conjugates of gold nanoparticles with streptococcal protein G were characterized by composition and Ig-binding properties. The optimal concentrations of protein G and gold nanoparticles with an average diameter of 25 nm were found to be 8μg/mL and 2.5 nM (OD₅₂₀ = 5), resp. The obtained conjugates were used for the developed immunochromatog. anal. of the antibiotic sulfonylamide as a relevant contaminant that is controlled in food products. Indirect labeling of specific antibodies allowed using crude rabbit antiserum in the anal. The visual and instrumental detection limits of sulfonylamide were 100 ng/mL and 0.3 ng/mL, resp. The time of the assay was 10 min. The developed assay was tested for the characterization of honey samples. In the experiment, the researchers used many compounds, for example, 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9COA of Formula: C17H10O4).

4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9) belongs to benzofurans derivatives. Benzofuran derivatives have shown many biological activities, including antifungal and antimicrobial properties, and acting as antagonists of H3 receptors and angiotensin II. Substituted benzofurans find applications such as fluorescent sensors, oxidants, in drug discovery, and in another field of chemistry and agriculture.COA of Formula: C17H10O4

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Dual-mode multicolored photonic crystal patterns enabled by ultraviolet-responsive core-shell colloidal spheres was written by Gao, Zewen;Huang, Chao;Yang, Dian;Zhang, Hanbing;Guo, Jinbao;Wei, Jie. And the article was included in Dyes and Pigments in 2018.Name: 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione This article mentions the following:

The authors developed a new kind of dual-mode multicolored photonic crystals (PCs), which could be used as a new type of dye, and achieved multicolored patterns. This kind of PCs can exhibit not only distinct structural colors due to Bragg’s law of diffraction but also nonstructural colors due to fluorescence. The authors used UV-responsive colloidal spheres synthesized by polymerization of semi-continuous emulsions to assemble the PCs. In this synthesis, the fluorescent precursors with carbamate groups were locally restricted to the shell of prepared spheres. UV irradiation can induce photo-decomposition of the carbamate groups in the fluorescent precursors, generating amino groups in the process. This process allowed the assembled PCs to exhibit fluorescence through treatment with fluorescamine. The authors prepared 2-dimensional (2D) multicolored photonic patterns of different sizes, with both reflection and fluorescence modes. These patterns were prepared either by vertical deposition under selective UV irradiation, or by spray coating. The authors fabricated 3-dimensional (3D) multicolored photonic patterns using 3D templates, as well as through spray coating. The results expand the concept of responsive PCs, and offer potential applications for multicolored photonic crystal patterns (PC patterns) in the field of optical storage, color displays, and anti-counterfeiting. In the experiment, the researchers used many compounds, for example, 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9Name: 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione).

4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9) belongs to benzofurans derivatives. Benzofuran derivatives are one of the most important oxygen-containing heterocycles. In nature, benzofurans have occupied an important role among the plant phenols & several pharmacologically active compounds.Name: 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Scaffold hopping and optimisation of 3′,4′-dihydroxyphenyl- containing thienopyrimidinones: synthesis of quinazolinone derivatives as novel allosteric inhibitors of HIV-1 reverse transcriptase-associated ribonuclease H was written by Tocco, Graziella;Esposito, Francesca;Caboni, Pierluigi;Laus, Antonio;Beutler, John A.;Wilson, Jennifer A.;Corona, Angela;Le Grice, Stuart F. J.;Tramontano, Enzo. And the article was included in Journal of Enzyme Inhibition and Medicinal Chemistry in 2020.Application In Synthesis of 3-Aminobenzofuran-2-carboxamide This article mentions the following:

Bioisosteric replacement and scaffold hopping are powerful strategies in drug design useful for rationally modifying a hit compound towards novel lead therapeutic agents. Recently, we reported a series of thienopyrimidinones that compromise dynamics at the p66/p51 HIV-1 reverse transcriptase (RT)-associated RNase H (RNase H) dimer interface, thereby allosterically interrupting catalysis by altering the active site geometry. Although they exhibited good submicromolar activity, the isosteric replacement of the thiophene ring, a potential toxicophore, is warranted. Thus, in this article, the most active 2-(3,4-dihydroxyphenyl)-5,6-dimethylthieno[2,3-d]pyrimidin-4(3H)-one was selected as the hit scaffold and several isosteric substitutions of the thiophene ring were performed. A novel series of highly active RNase H allosteric quinazolinone inhibitors was thus obtained. To determine their target selectivity, they were tested against RT-associated RNA-dependent DNA polymerase (RDDP) and integrase (IN). Interestingly, none of the compounds were particularly active on (RDDP) but many displayed micromolar to submicromolar activity against IN. In the experiment, the researchers used many compounds, for example, 3-Aminobenzofuran-2-carboxamide (cas: 54802-10-7Application In Synthesis of 3-Aminobenzofuran-2-carboxamide).

3-Aminobenzofuran-2-carboxamide (cas: 54802-10-7) belongs to benzofurans derivatives.Benzofuran is one of the most significant oxygen-containing heterocycles consisting of fused benzene and furan ring, which are widely presented in various naturally occurring and synthetically active compounds. Benzofurans are stable towards alkali and readily polymerize on treatment with sulfuric acid, due to which they are useful for the preparation of low cost chemically relatively inert resins.Application In Synthesis of 3-Aminobenzofuran-2-carboxamide

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Kinetics of bromination of benzofurans by phenyltrimethylammonium tribromide was written by Suryawanshi, V. S.. And the article was included in Journal of Chemical and Pharmaceutical Research in 2017.Formula: C10H7BrO2 This article mentions the following:

The kinetics of the reaction between different 2-acetyl benzofuran (BF) and phenyltrimethylammonium tribromide (PTT) has been studied spectrophotometrically in acetic acid under varying conditions. The results indicate first order kinetic with respect to 2-acetyl benzofurans (BF) and inverse first order due to phenyltrimethylammonium tribromide (PTT). Effect of ionic strength and dielec. constant on reaction rate was observed The rates of reaction are measured at different temperature and activation parameters have been calculated The products of the bromination are the corresponding 2-bromoacetyl benzofurans. In the experiment, the researchers used many compounds, for example, 1-(5-Bromobenzofuran-2-yl)ethanone (cas: 38220-75-6Formula: C10H7BrO2).

1-(5-Bromobenzofuran-2-yl)ethanone (cas: 38220-75-6) belongs to benzofurans derivatives. Benzofuran is a core structural unit found in many naturally occurring compounds with multidirectional biological activities. Introduction of benzofurans in organic synthesis, particularly drug synthesis, involves generally the use of their metalated species as nucleophiles in addition reactions or in metal-catalysed cross-coupling reactions.Formula: C10H7BrO2

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem