Tag: 200-300

Threads for tear film collection and support in quantitative amino acid analysis was written by Avilov, Vitaly;Zeng, Qi;Shippy, Scott A.. And the article was included in Analytical and Bioanalytical Chemistry in 2016.HPLC of Formula: 38183-12-9 This article mentions the following:

The collection of tears for chem. composition anal. is complicated by both the difficulty in sampling the tear film and the relatively low microliter volumes available for anal. The experiments in this study are focused on the demonstration of a method for determining amino acids from tear samples. Phenol red thread was used to absorptively collect tear fluid for qual. and quant. analyses of amino acids in basal, reflex, and emotional tears. The thread is also used as a support for sample preparation followed by elution with a buffer. The phenol red indicator on the thread turns from yellow to red with 15-s tear absorption and allows accurate volume measurement from 100 nL to over 1 μL. Derivatization of amino acids was performed directly on the thread with primary amine reactive fluorescamine for fluorescence detection. Analyte elution was performed via centrifugation with the thread in a pipet tip suspended in a centrifuge tube. Collected tear eluate was analyzed via capillary electrophoresis with LED-induced fluorescence. Glycine, glutamate, and aspartate were baseline resolved and used for method characterization. Recoveries were at 50 % for a single derivation and elution step but average recoveries near 90 % were found with two-step processing. Glutamate and aspartate are shown to be stable stored on thread for 3 days. Basal, reflex, and emotional tears were analyzed from three subjects showing distinct amino acid profiles for each tear type. The demonstration of this method may facilitate the development of routine tear compositional anal. to assess ocular health. [Figure not available: see fulltext.]. In the experiment, the researchers used many compounds, for example, 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9HPLC of Formula: 38183-12-9).

4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9) belongs to benzofurans derivatives. Benzofurans are only weakly aromatic in nature and they are cleaved by many oxidative and reductive conditions. Substituted benzofurans find applications such as fluorescent sensors, oxidants, in drug discovery, and in another field of chemistry and agriculture.HPLC of Formula: 38183-12-9

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Quantification of Free Polyelectrolytes Present in Colloidal Suspension, Revealing a Source of Toxic Responses for Polyelectrolyte-Wrapped Gold Nanoparticles was written by Qiu, Tian A.;Torelli, Marco D.;Vartanian, Ariane M.;Rackstraw, Nathan B.;Buchman, Joseph T.;Jacob, Lisa M.;Murphy, Catherine J.;Hamers, Robert J.;Haynes, Christy L.. And the article was included in Analytical Chemistry (Washington, DC, United States) in 2017.Application of 38183-12-9 This article mentions the following:

Polyelectrolyte (PE) wrapping of colloidal nanoparticles (NPs) is a standard method to control NP surface chem. and charge. As excess polyelectrolytes are usually employed in the surface modification process, it is critical to evaluate different purification strategies to obtain a clean final product and thus avoid ambiguities in the source of effects on biol. systems. In this work, 4-nm-diameter gold nanoparticles (AuNPs) were wrapped with 15 kDa poly(allylamine hydrochloride) (PAH), and three purification strategies were applied: (a) diafiltration or either (b) one round or (c) two rounds of centrifugation. The bacterial toxicity of each of these three PAH-AuNP samples was evaluated for the bacterium Shewanella oneidensis MR-1 and is quant. correlated with the amount of unbound PAH mols. in the AuNP suspensions, as judged by XPS and NMR experiments Dialysis experiments show that for a 15 kDa polyelectrolyte, a 50 kDa dialysis membrane is not sufficient to remove all unbound PAH polymers from a nanoparticle suspension. Together, these data showcase the importance of choosing a proper post-synthesis purification method for polyelectrolyte-wrapped NPs and reveal that apparent toxicity results may be due to unintended free wrapping agents like polyelectrolytes. In the experiment, the researchers used many compounds, for example, 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9Application of 38183-12-9).

4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9) belongs to benzofurans derivatives. Benzofuran derivatives are one of the most important oxygen-containing heterocycles. Benzofurans have also made significant and distinctive contributions to biology. They exhibit several biological activities that range from antiviral, antimicrobial, antitumor, anti-inflammatory.Application of 38183-12-9

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Synthesis and Evaluation of Antimicrobial Activity of Some Novel Heterocyclic Compounds from 5-Bromosalicylaldehyde was written by Abdel-Aziem, Anhar;Baaiu, Basma S.;Abdelhamid, Abdou O.. And the article was included in Journal of Heterocyclic Chemistry in 2017.Product Details of 38220-75-6 This article mentions the following:

A novel series of coumarins, thiadiazoles, thiazoles, and pyridines were synthesized via reaction of 5-bromosalicylaldehyde with different reagents. Thus, 5-bromosalicylaldehyde was reacted with acetonitrile derivatives affording iminocoumarins, which on hydrolysis with 10% hydrochloric acid, afforded coumarins. On the other hand, reaction of 5-bromosalicylaldehyde with benzylhydrazinecarbodithioate afforded a carbodithioate, which reacted with hydrazonoyl halides, affording 1,3,4-thiadiazoles. Moreover, thiazoles were obtained via reaction of 5-bromosalicylaldehyde with a thiocarbohydrazide derivative and hydrazonoyl halides. However, condensation of 2-acetyl-5-bromobenzofuran with benzaldehyde afforded a chalcone, which reacted with pyridinium bromides to afford pyridine derivatives (I, R = Ph, pMePh, 5-bromobenzofuran-2-yl). Furthermore, pyridines were synthesized from the reaction of a chalcone with different active methylene compounds Reaction of II with ethylchloroacetate, chloroacetone, and chloroacetonitrile afforded thienopyridines (III, Y = CO₂Et, Ac, CN). The structures of the newly synthesized compounds were established based on their spectral data and elemental analyses. Also, selected newly synthesized compounds were screened for their antimicrobial activity against various microorganisms by disk diffusion method. In the experiment, the researchers used many compounds, for example, 1-(5-Bromobenzofuran-2-yl)ethanone (cas: 38220-75-6Product Details of 38220-75-6).

1-(5-Bromobenzofuran-2-yl)ethanone (cas: 38220-75-6) belongs to benzofurans derivatives. Benzofurans are only weakly aromatic in nature and they are cleaved by many oxidative and reductive conditions. As benzofurans are prone to undergo ring opening of the heterocycle, examples of reduction of this type of aromatics by using dissolving metals are rather scarce.Product Details of 38220-75-6

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Acidity-triggered TAT-presenting nanocarriers augment tumor retention and nuclear translocation of drugs was written by Jiang, Wei;Wang, Jilong;Yang, Jinbin;He, Zhiwei;Hou, Zhenhui;Luo, Yingli;Wang, Li;Liu, Jing;Zhang, Houbing;Zhao, Yangyang;Zhang, Guoqing;Huang, Fang;Zhou, Xuechang;Yan, Lifeng;Yang, Xianzhu;Wang, Yucai;Wang, Jun. And the article was included in Nano Research in 2018.HPLC of Formula: 38183-12-9 This article mentions the following:

In this work, we demonstrate that a transactivator of transcription (TAT)-presenting nanomedicine (DATAT-NP/Pt), apart from improving tumor accumulation and cellular uptake, can simultaneously enhance tumor retention and promote nuclear translocation of encapsulated platinum prodrugs, and thus improve therapeutic efficacy. Specifically, a protecting 2,3-dimethylmaleic anhydride (DA) corona on the nanomedicine prevented the TAT peptide from serum. DATAT-NP/Pt efficiently accumulated at the tumor site through the enhanced permeability and retention (EPR) effect, followed by acid-triggered TAT presenting within the tumor acidic microenvironment (pH ~6.8). The exposed TAT peptide augmented tumor retention and nuclear translocation of DATAT-NP/Pt. We used a tumor-on-a-chip microfluidic system to real-time mimic and analyze tumor accumulation and retention at physiol. flow conditions and revealed that surface absorption of nanomedicines on tumors was critical in determining their tumor retention and clearance. Furthermore, the TAT peptide rapidly translocated the DATAT-NP/Pt into the perinuclear region, allowing for higher nuclear platinum concentrations and increased Pt-DNA adduct formation in nuclei, which consequently reversed cisplatin resistance. Our work presents a new strategy to overcome pathophysiol. barriers of tumor clearance and insufficient cellular translocation and provides new insights for the design of cancer nanomedicines. In the experiment, the researchers used many compounds, for example, 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9HPLC of Formula: 38183-12-9).

4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9) belongs to benzofurans derivatives. Benzofuran derivatives are one of the most important oxygen-containing heterocycles. They are also prone to polymerisation in the presence of concentrated mineral acids and Lewis acids.HPLC of Formula: 38183-12-9

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

New chemical tools for probing activity and inhibition of the NAD+-dependent lysine deacylase sirtuin 2 was written by Swyter, Soeren;Schiedel, Matthias;Monaldi, Daria;Szunyogh, Sandor;Lehotzky, Attila;Rumpf, Tobias;Ova’di, Judit;Sippl, Wolfgang;Jung, Manfred. And the article was included in Philosophical Transactions of the Royal Society, B: Biological Sciences in 2018.Safety of 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione This article mentions the following:

Sirtuins are NAD+-dependent protein deacylases capable of cleaving off acetyl as well as other acyl groups from the 1-amino group of lysines in histones and other substrate proteins. They have been reported as promising drug targets, and thus modulators of their activity are needed as mol. tools to uncover their biol. function and as potential therapeutics. Here, we present new assay formats that complement existing assays for sirtuin biochem. and cellular target engagement. Firstly, we report the development of a homogeneous fluorescence-based activity assay using unlabeled acylated peptides. Upon deacylation, the free lysine residue reacts with fluorescamine to form a fluorophore. Secondly, using click chem. with a TAMRA-azide on a propargylated sirtuin inhibitor, we prepared the first fluorescently labeled small-mol. inhibitor of Sirt2. This is used in a binding assay, which is based on fluorescence polarization. We used it successfully to map potential inhibitor-binding sites and also to show cellular Sirt2 engagement. By means of these new assays, we were able to identify and characterize novel Sirt2 inhibitors out of a focused library screen. The binding of the identified Sirt2 inhibitors was rationalized by mol. docking studies. These new chem. tools thus can enhance further sirtuin research. In the experiment, the researchers used many compounds, for example, 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9Safety of 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione).

4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9) belongs to benzofurans derivatives. Benzofurans are compounds with a planar structure having 10 pi electrons that include the lone pair on oxygen atom, which makes it more susceptible to electrophilic attack. Introduction of benzofurans in organic synthesis, particularly drug synthesis, involves generally the use of their metalated species as nucleophiles in addition reactions or in metal-catalysed cross-coupling reactions.Safety of 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Determination of eleven sulfonamides residues in animal foods by high performance liquid chromatography-fluorescence detection after matrix solid phase dispersion extraction was written by Yang, Xiaosong;Yu, Huiju;Qiu, Sha;Sun, Chengjun. And the article was included in Zhongguo Weisheng Jianyan Zazhi in 2013.Application of 38183-12-9 This article mentions the following:

A method for simultaneous determination of eleven sulfonamide residues (SAs), including sulfisomidine, sulfapyridine, sulfadimidin, sulfamoxol, sulfamonomethoxine, sulfamethizol, sulfachloyopyridazine, sulfasoxine, sulfamethoxazole, sulfisoxazole, and sulfabeuzamide, in animal foods by high-performance liquid chromatog. (HPLC) with fluorescence detection using matrix solid-phase dispersion (MSPD) extraction was presented. Animal food sample was crushed and well-distributed with the solid-phase sorbents C₁₈, and then the mixture was loaded in a vacant SPE cartridge, covered with a piece of filter paper on the top of the mixture, rinsed with hexane to remove fat, eluted with 3:1 (volume/volume) Et acetate-dichloromethane solvent to elute the analytes, then the eluant was dried up at 40°C under N₂, and finally the residue was re-dissolved with 1.0 mL 1:1 (volume/volume) methanol-0.2% acetic acid, fluorescamine was added to produce fluorescent derivatives The derivatives were determined by HPLC with fluorescence detection, using an Agilent TC-C₁₈ column and a mixture of 0.2% acetic acid solution-acetonitrile as the mobile phase for gradient elution and detected with a fluorescence detector. The linear ranges of eleven sulfonamides were between 0.02 mg/ to 0.40 mg/L with r>0.999. The detection limits were 0.001 mg/L. The min. detectable content was 0.006 mg/kg. The recovery range was between 54% and 105% with relative standard deviations from 1.3% to 6.1%. The method has the advantages of a small amount of organic solvent, short time of anal., high sensitivity, and low detection limit. In the experiment, the researchers used many compounds, for example, 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9Application of 38183-12-9).

4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9) belongs to benzofurans derivatives. Benzofurans are only weakly aromatic in nature and they are cleaved by many oxidative and reductive conditions. Benzofurans are stable towards alkali and readily polymerize on treatment with sulfuric acid, due to which they are useful for the preparation of low cost chemically relatively inert resins.Application of 38183-12-9

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

A review on the use of fluorescamine as versatile and convenient analytical probe was written by Derayea, Sayed M.;Samir, Ebtihal. And the article was included in Microchemical Journal in 2020.COA of Formula: C17H10O4 This article mentions the following:

Fluorescamine (FSA) has been widely applied for the determination of several classes of compounds containing amino groups. The estimated substances included pharmaceutical compounds, pesticides, amino acids, peptides and proteins. In addition, the activities of some enzymes were also monitored by FSA assay. The employed techniques mainly included spectrofluorimetry, as spray reagent in TLC, a derivativzation reagent in HPLC and capillary electrophoresis. Although there are many other reagents for detecting primary amino group containing compounds, FSA is advantageous as the reagent itself and its hydrolytic product are non-fluorescent. While other reagents such as NBD-Cl, dansyl chloride and o-phthaldehyde have a native fluorescence and their hydrolytic products are indeed fluorescent. In addition, the involved reaction is very fast and carried out at ambient temperature However, the sensitivity of FSA anal. is much lower than these reagents. The sensitivity of the reagent could be enhanced by the proper selection of the diluting solvent, addition of surfactants or extraction with water immiscible solvent. The present article represents a comprehensive review for the application of FSA as a reagent in chem. and biochem. anal. since its discovery until now. In the experiment, the researchers used many compounds, for example, 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9COA of Formula: C17H10O4).

4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9) belongs to benzofurans derivatives. Benzofurans are only weakly aromatic in nature and they are cleaved by many oxidative and reductive conditions. Introduction of benzofurans in organic synthesis, particularly drug synthesis, involves generally the use of their metalated species as nucleophiles in addition reactions or in metal-catalysed cross-coupling reactions.COA of Formula: C17H10O4

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

A self-assembly pipette tip graphene solid-phase extraction coupled with liquid chromatography for the determination of three sulfonamides in environmental water was written by Sun, Ning;Han, Yehong;Yan, Hongyuan;Song, Yanxue. And the article was included in Analytica Chimica Acta in 2014.Name: 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione This article mentions the following:

A sensitive, economical, and miniaturized self-assembly pipet tip graphene solid-phase extraction (PT-G-SPE) coupled with liquid chromatog. fluorescence detection (LC-FD) was developed for rapid extraction and determination of three sulfonamide antibiotics (SAs) in environmental water samples. The PT-G-SPE cartridge, assembled by packing 1.0 mg of graphene as sorbent into a 100 μL pipet tip, showed high adsorption capacity for the SAs owing to the large surface area and unique structure of graphene. The factors that affected the extraction efficiency of PT-G-SPE, including sample volume, pH, sorbent amount, washing solvent and eluent solvent were optimized. Good linearity for SAs was obtained in a range of 2-4000 pg mL^-1 with correlation coefficients (r²) ≥0.9993. The recoveries of the SAs at three spiked levels ranged from 90.4 to 108.2% with RSD ≤6.3%. In comparison with other sorbents such as C₁₈, HLB, SCX, PCX, and multiwalled carbon nanotubes, one advantage of using graphene as sorbent of pipet tip solid-phase extraction (PT-SPE) was that PT-G-SPE could adsorb larger sample volume (10 mL) at a small amount of sorbent (1 mg) and low solvent consumption with good extraction efficiency, which not only increased the fraction of analytes to LC and the sensitivity of SAs determination, but also reduced the cost and pollution. In the experiment, the researchers used many compounds, for example, 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9Name: 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione).

4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9) belongs to benzofurans derivatives. Benzofuran derivatives are one of the most important oxygen-containing heterocycles. Introduction of benzofurans in organic synthesis, particularly drug synthesis, involves generally the use of their metalated species as nucleophiles in addition reactions or in metal-catalysed cross-coupling reactions.Name: 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Determination of clenbuterol hydrochloride in pork by fluorescence spectrophotometry was written by Gao, Xianjuan. And the article was included in Zhongguo Niangzao in 2014.Application of 38183-12-9 This article mentions the following:

Clenbuterol hydrochloride in pork was determined with high fluorescent derivatives generated from fluorescamine and clenbuterol hydrochloride. Experiments showed that the maximum excitation wavelength and maximum emission wavelength of the strong fluorescent material produced by fluorescence amine and clenbuterol reaction were 500 nm and 490 nm, resp. The optimum derivative test conditions were determined by L₉ (3⁴) orthogonal experiments as follows: derivative temperature 37°C, time 40 min, and fluorescamine (1 μg/mL) addition 0.3 mL. The fluorescence signal was in good linear relation with clenbuterol hydrochloride concentration, and the linear equation was y=82492x+957.96 and the correlation coefficient was 0.9933. The method could be used in clenbuterol detection in pork. In the experiment, the researchers used many compounds, for example, 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9Application of 38183-12-9).

4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9) belongs to benzofurans derivatives. Many natural or synthetic compounds containing benzofuran skeletons have been found to possess remarkable activity as agrochemicals and pharmaceuticals. Benzofurans have also made significant and distinctive contributions to biology. They exhibit several biological activities that range from antiviral, antimicrobial, antitumor, anti-inflammatory.Application of 38183-12-9

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

A lab-on-a-chip device for analysis of amlodipine in biological fluids using peroxyoxalate chemiluminescence system was written by Al Lawati, Haider A. J.;Al-Nadabi, Mira M.;Varma, Gouri B.;Suliman, Fakhr Eldin O.;Al-Abri, Hasnaa. And the article was included in Luminescence in 2014.Computed Properties of C17H10O4 This article mentions the following:

A highly sensitive, rapid and economical method for the determination of amlodipine (AM) in biol. fluids was developed using a peroxyoxalate chemiluminescence (CL) system in a lab-on-a-chip device. Peroxyoxalate-CL is an indirect type of CL that allows the detection of native fluorophores or compounds derivatized with fluorescent labels. Here, fluorescamine was reacted with AM, and the derivatization product was used in a bis-(2,4,6-trichlorophenyl)oxalate-CL system. Fluorescamine reacts selectively with aliphatic primary amine at neutral or basic pH. As most of the calcium channel blocker and many cardiovascular drugs do not contain primary amine, the developed method is highly selective. The parameters that influenced the CL signal intensity were studied carefully. These included the chip geometry, pH, concentration of reagents used and flow rates. Moreover, we confirmed our previous observation about the effects of imidazole, which is commonly used in the bis-(2,4,6-trichlorophenyl)oxalate-CL system as a catalyst, and found that the signal was significantly improved when imidazole was absent. Under optimized conditions, a calibration curve was obtained with a linear range (10-100 μg/L). The limit of detection was 3 μg/L, while the limit of quantification was 10 μg/L. Finally the method was applied for the determination of AM in biol. fluids successfully. Copyright © 2014 John Wiley & Sons, Ltd. In the experiment, the researchers used many compounds, for example, 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9Computed Properties of C17H10O4).

4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9) belongs to benzofurans derivatives. Benzofurans are only weakly aromatic in nature and they are cleaved by many oxidative and reductive conditions. As benzofurans are prone to undergo ring opening of the heterocycle, examples of reduction of this type of aromatics by using dissolving metals are rather scarce.Computed Properties of C17H10O4

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem