Determination of clenbuterol hydrochloride in pork by fluorescence spectrophotometry was written by Gao, Xianjuan. And the article was included in Zhongguo Niangzao in 2014.Application of 38183-12-9 This article mentions the following:
Clenbuterol hydrochloride in pork was determined with high fluorescent derivatives generated from fluorescamine and clenbuterol hydrochloride. Experiments showed that the maximum excitation wavelength and maximum emission wavelength of the strong fluorescent material produced by fluorescence amine and clenbuterol reaction were 500 nm and 490 nm, resp. The optimum derivative test conditions were determined by L9 (34) orthogonal experiments as follows: derivative temperature 37°C, time 40 min, and fluorescamine (1 μg/mL) addition 0.3 mL. The fluorescence signal was in good linear relation with clenbuterol hydrochloride concentration, and the linear equation was y=82492x+957.96 and the correlation coefficient was 0.9933. The method could be used in clenbuterol detection in pork. In the experiment, the researchers used many compounds, for example, 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9Application of 38183-12-9).
4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9) belongs to benzofurans derivatives. Many natural or synthetic compounds containing benzofuran skeletons have been found to possess remarkable activity as agrochemicals and pharmaceuticals. Benzofurans have also made significant and distinctive contributions to biology. They exhibit several biological activities that range from antiviral, antimicrobial, antitumor, anti-inflammatory.Application of 38183-12-9
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem