Electrochemical Detection of Ammonia in Aqueous Solution Using Fluorescamine: A Comparison of Fluorometric Versus Voltammetric Analysis was written by Panchompoo, Janjira;Compton, Richard G.. And the article was included in Dianhuaxue in 2012.Safety of 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione This article mentions the following:
Fluorescamine is a non-fluorescent reagent widely used for the quant. determination o f primary amines by fluorescence spectroscopy as it reacts readily with primary amines to form a fluorescent product. In this work, a new sensitive voltammetric method for the detection of ammonia in aqueous solution by the reaction with fluorescamine was developed. First, the electrochem. behavior of fluorescamine in the absence and presence o f ammonia was investigated in 0.1 mol·L-1 borate buffer solution (pH 9.0) by cyclic voltammetry using a glassy carbon (GC) electrode. As for fluorescamine itself, a well-defined irreversible oxidation peak could be observed at ∼0.70 V vs. SCE. When ammonia was added to the fluorescamine solution, another irreversible oxidation peak corresponding to the oxidation o f the reaction product formed between fluorescamine and ammonia could be observed at ∼0.46 V vs. SCE. Upon the addition of ammonia, the oxidation peak o f fluorescamine became smaller while the oxidation peak o f the reaction product formed increased in height, due to the stoichiometric chem. consumption o f fluorescamine by ammonia and the formation o f the product during the reaction, resp. These two anodic peaks corresponding to the oxidation of fluorescamine and its fluorescent product formed were then used for the quant. detection o f ammonia, explored by square wave voltammetry and by fluorescence spectroscopy. The square wave voltammetric response o f the reaction product formed showed a linear response over ammonia concentration range o f 0 to 60 μmol-L-1. The limits o f detection (LOD) was 0.71 μmol·L-1 and 3.17 μmol-L-1 determined based upon Signal/Noise (S/N) = 3 and 3cr, resp. These limits o f detection are similar to those obtained with the fluorometric method. In the experiment, the researchers used many compounds, for example, 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9Safety of 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione).
4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9) belongs to benzofurans derivatives. Benzofuran is a core structural unit found in many naturally occurring compounds with multidirectional biological activities. Benzofurans are stable towards alkali and readily polymerize on treatment with sulfuric acid, due to which they are useful for the preparation of low cost chemically relatively inert resins.Safety of 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem