Category: 92557-80-7

Specific Cell Surface Protein Imaging by Extended Self-Assembling Fluorescent Turn-on Nanoprobes was written by Mizusawa, Keigo;Takaoka, Yousuke;Hamachi, Itaru. And the article was included in Journal of the American Chemical Society in 2012.Application of 92557-80-7 This article mentions the following:

Visualization of tumor-specific protein biomarkers on cell membranes has the potential to contribute greatly to basic biol. research and therapeutic applications. The authors recently reported a unique supramol. strategy for specific protein detection using self-assembling fluorescent nanoprobes consisting of a hydrophilic protein ligand and a hydrophobic BODIPY fluorophore in test tube settings. This method is based on recognition-driven disassembly of the nanoprobes, which induces a clear turn-on fluorescent signal. The authors have successfully extended the range of applicable fluorophores to the more hydrophilic ones such as fluorescein or rhodamine by introducing a hydrophobic module near the fluorophore. Increasing the range of available fluorophores allowed selective imaging of membrane-bound proteins under live cell conditions. That is, overexpressed folate receptor (FR) or hypoxia-inducible membrane-bound carbonic anhydrases (CA) on live cell surfaces as cancer-specific biomarkers were fluorescently visualized using the designed supramol. nanoprobes in the turn-on manner. Moreover, a cell-based inhibitor-assay platform for CA on a live cell surface was constructed, highlighting the potential applicability of the self-assembling turn-on probes. In the experiment, the researchers used many compounds, for example, 2,5-Dioxopyrrolidin-1-yl 3′,6′-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-5-carboxylate (cas: 92557-80-7Application of 92557-80-7).

2,5-Dioxopyrrolidin-1-yl 3′,6′-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-5-carboxylate (cas: 92557-80-7) belongs to benzofurans derivatives. Benzofuran is the “”parent”” of many related compounds with more complex structures. For example, psoralen is a benzofuran derivative that occurs in several plants. Benzofurans have also made significant and distinctive contributions to biology. They exhibit several biological activities that range from antiviral, antimicrobial, antitumor, anti-inflammatory.Application of 92557-80-7

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Small molecule probes to quantify the functional fraction of a specific protein in a cell with minimal folding equilibrium shifts was written by Liu, Yu;Tan, Yun Lei;Zhang, Xin;Bhabha, Gira;Ekiert, Damian C.;Genereux, Joseph C.;Cho, Younhee;Kipnis, Yakov;Bjelic, Sinisa;Baker, David;Kelly, Jeffery W.. And the article was included in Proceedings of the National Academy of Sciences of the United States of America in 2014.Application In Synthesis of 2,5-Dioxopyrrolidin-1-yl 3′,6′-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-5-carboxylate This article mentions the following:

Although much is known about protein folding in buffers, it remains unclear how the cellular protein homeostasis network functions as a system to partition client proteins between folded and functional, soluble and misfolded, and aggregated conformations. Herein, we develop small mol. folding probes that specifically react with the folded and functional fraction of the protein of interest, enabling fluorescence-based quantification of this fraction in cell lysate at a time point of interest. Importantly, these probes minimally perturb a protein’s folding equilibrium within cells during and after cell lysis, because sufficient cellular chaperone/chaperonin holdase activity is created by rapid ATP depletion during cell lysis. The folding probe strategy and the faithful quantification of a particular protein’s functional fraction are exemplified with retroaldolase, a de novo designed enzyme, and transthyretin, a nonenzyme protein. Our findings challenge the often invoked assumption that the soluble fraction of a client protein is fully folded in the cell. Moreover, our results reveal that the partitioning of destabilized retroaldolase and transthyretin mutants between the aforementioned conformational states is strongly influenced by cytosolic proteostasis network perturbations. Overall, our results suggest that applying a chem. folding probe strategy to other client proteins offers opportunities to reveal how the proteostasis network functions as a system to regulate the folding and function of individual client proteins in vivo. In the experiment, the researchers used many compounds, for example, 2,5-Dioxopyrrolidin-1-yl 3′,6′-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-5-carboxylate (cas: 92557-80-7Application In Synthesis of 2,5-Dioxopyrrolidin-1-yl 3′,6′-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-5-carboxylate).

2,5-Dioxopyrrolidin-1-yl 3′,6′-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-5-carboxylate (cas: 92557-80-7) belongs to benzofurans derivatives. Benzofuran is a core structural unit found in many naturally occurring compounds with multidirectional biological activities. Substituted benzofurans find applications such as fluorescent sensors, oxidants, in drug discovery, and in another field of chemistry and agriculture.Application In Synthesis of 2,5-Dioxopyrrolidin-1-yl 3′,6′-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-5-carboxylate

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Protein-responsive protein release of supramolecular/polymer hydrogel composite integrating enzyme activation systems was written by Shigemitsu, Hajime;Kubota, Ryou;Nakamura, Keisuke;Matsuzaki, Tomonobu;Minami, Saori;Aoyama, Takuma;Urayama, Kenji;Hamachi, Itaru. And the article was included in Nature Communications.Synthetic Route of C25H15NO9 This article mentions the following:

Non-enzymic proteins including antibodies function as biomarkers and are used as biopharmaceuticals in several diseases. Protein-responsive soft materials capable of the controlled release of drugs and proteins have potential for use in next-generation diagnosis and therapies. Here, we describe a supramol./agarose hydrogel composite that can release a protein in response to a non-enzymic protein. A non-enzymic protein-responsive system is developed by hybridization of an enzyme-sensitive supramol. hydrogel with a protein-triggered enzyme activation set. In situ imaging shows that the supramol./agarose hydrogel composite consists of orthogonal domains of supramol. fibers and agarose, which play distinct roles in protein entrapment and mech. stiffness, resp. Integrating the enzyme activation set with the composite allows for controlled release of the embedded RNase in response to an antibody. Such composite hydrogels would be promising as a matrix embedded in a body, which can autonomously release biopharmaceuticals by sensing biomarker proteins. In the experiment, the researchers used many compounds, for example, 2,5-Dioxopyrrolidin-1-yl 3′,6′-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-5-carboxylate (cas: 92557-80-7Synthetic Route of C25H15NO9).

2,5-Dioxopyrrolidin-1-yl 3′,6′-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-5-carboxylate (cas: 92557-80-7) belongs to benzofurans derivatives. Benzofurans are only weakly aromatic in nature and they are cleaved by many oxidative and reductive conditions. Benzofurans have also made significant and distinctive contributions to biology. They exhibit several biological activities that range from antioxidant, antitubercular, antiplasmodial, insecticidal.Synthetic Route of C25H15NO9

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Design and synthesis of fluorescent SGLT2 inhibitors was written by Lansdell, Mark I.;Burring, Denise J.;Hepworth, David;Strawbridge, Matthew;Graham, Emily;Guyot, Thierry;Betson, Mark S.;Hart, James D.. And the article was included in Bioorganic & Medicinal Chemistry Letters in 2008.Product Details of 92557-80-7 This article mentions the following:

The design and synthesis of the first fluorophore-conjugated SGLT2 inhibitors is described. The mode of linking the fluorophore to the SGLT2 pharmacophore was crucial in achieving optimum potency. Superior potency to phlorizin was provided by examples containing TAMRA, BODIPY, Cy3B and NBD fluorophores. In the experiment, the researchers used many compounds, for example, 2,5-Dioxopyrrolidin-1-yl 3′,6′-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-5-carboxylate (cas: 92557-80-7Product Details of 92557-80-7).

2,5-Dioxopyrrolidin-1-yl 3′,6′-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-5-carboxylate (cas: 92557-80-7) belongs to benzofurans derivatives. Benzofuran is a core structural unit found in many naturally occurring compounds with multidirectional biological activities. Benzofurans have also made significant and distinctive contributions to biology. They exhibit several biological activities that range from antioxidant, antitubercular, antiplasmodial, insecticidal.Product Details of 92557-80-7

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Development of a temperature gradient focusing method for in situ extraterrestrial biomarker analysis was written by Danger, Gregoire;Ross, David. And the article was included in Electrophoresis in 2008.Application of 92557-80-7 This article mentions the following:

Scanning temperature gradient focusing (TGF) is a recently described technique for the simultaneous concentration and separation of charged analytes. It allows for high analyte peak capacities and low LODs in microcolumn electrophoretic separations In this paper, the authors present the application of scanning TGF for chiral separations of amino acids. Using a mixture of seven carboxyfluorescein succinimidyl ester-labeled amino acids (including five chiral amino acids) which constitute the Mars7 standard, the authors show that scanning TGF is a very simple and efficient method for chiral separations The modulation of TGF separation parameters (temperature window, pressure scan rate, temperature range, and chiral selector concentration) allows optimization of peak efficiencies and analyte resolutions The use of hydroxypropyl-β-cyclodextrin at low concentration (1-5 mmol/L) as a chiral selector, with an appropriate pressure scan rate (-0.25 Pa/s) and with a low temperature range (3-25° over 1 cm) provided high resolution between enantiomers (R_s >1.5 for each pair of enantiomers) using a short, 4 cm long capillary. With these new results, the scanning TGF method appears to be a viable method for in situ trace biomarker anal. for future missions to Mars or other solar system bodies. In the experiment, the researchers used many compounds, for example, 2,5-Dioxopyrrolidin-1-yl 3′,6′-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-5-carboxylate (cas: 92557-80-7Application of 92557-80-7).

2,5-Dioxopyrrolidin-1-yl 3′,6′-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-5-carboxylate (cas: 92557-80-7) belongs to benzofurans derivatives.Benzofuran is one of the most significant oxygen-containing heterocycles consisting of fused benzene and furan ring, which are widely presented in various naturally occurring and synthetically active compounds. Benzofurans have also made significant and distinctive contributions to biology. They exhibit several biological activities that range from antiviral, antimicrobial, antitumor, anti-inflammatory.Application of 92557-80-7

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Simultaneous Sensitive MEKC-LIF Determination of Homocysteine, Homoarginine, and Six Arginine Metabolic Derivatives in Fluids from Type 2 Diabetics with Peptic Ulcer Bleeding was written by Liang, Qianping;Chen, Hongchao;Li, Fuqing;Du, Xiaolin. And the article was included in Chromatographia in 2015.Computed Properties of C25H15NO9 This article mentions the following:

The suitability of micellar electrokinetic capillary chromatog. (MEKC) with laser-induced fluorescence (LIF) detection for simultaneous determination of two arginine analogs (homocysteine and homoarginine) and five closely related metabolites (asym. dimethyl-L-arginine, dimethyl-L-arginine, monomethyl-L-arginine, citrulline, and ornithine) in fluids from type 2 diabetics with peptic ulcer bleeding (PUB) has been studied. 5-Carboxyfluorescein succinimidyl ester (CFSE) was chosen as the fluorescent labeling reagent and non-endogenous phenylpropanolamine (PPA) as the internal standard Conditions affecting derivatization and separation were optimized. Under the optimum conditions, maximum derivatization could be achieved in 20 min at room temperature Complete baseline separation was achieved in 10 min, and the relative standard deviations (relative standard deviation) of migration times and corrected peak areas were <3% for intra-day assay and <5% for inter-day assay. Limits of detection (LODs) were 0.12-1.70 nM for the eight analytes, which are well below the concentrations expected in real fluids. Compared with previously reported methods, 5 to 600-fold improvements in sensitivity were achieved using LIF detection. Sample preparation and anal. time were short and the derivatives of the analytes were highly stable. The method was fully validated with real plasma and urine and recoveries of spiked compounds were 95-102% with the relative standard deviation <4%. In the experiment, the researchers used many compounds, for example, 2,5-Dioxopyrrolidin-1-yl 3′,6′-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-5-carboxylate (cas: 92557-80-7Computed Properties of C25H15NO9).

2,5-Dioxopyrrolidin-1-yl 3′,6′-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-5-carboxylate (cas: 92557-80-7) belongs to benzofurans derivatives. Benzofuran derivatives have shown many biological activities, including antifungal and antimicrobial properties, and acting as antagonists of H3 receptors and angiotensin II. Introduction of benzofurans in organic synthesis, particularly drug synthesis, involves generally the use of their metalated species as nucleophiles in addition reactions or in metal-catalysed cross-coupling reactions.Computed Properties of C25H15NO9

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Monitoring the site-specific incorporation of dual fluorophore-quencher base analogues for target DNA detection by an unnatural base pair system was written by Yamashige, Rie;Kimoto, Michiko;Mitsui, Tsuneo;Yokoyama, Shigeyuki;Hirao, Ichiro. And the article was included in Organic & Biomolecular Chemistry in 2011.Application of 92557-80-7 This article mentions the following:

We developed intramol. dual fluorophore-quencher base analogs for site-specific incorporation into DNA by an unnatural base pair replication system. An unnatural base pair between 7-(2-thienyl)-imidazo[4,5-b]pyridine (Ds) and 2-nitro-4-propynylpyrrole (Px) exhibits high fidelity in PCR amplification, and the 2-nitropyrrole moiety of Px acts as a quencher. Deoxyribonucleoside triphosphates of Px linked with a fluorophore (Cy3, Cy5 or FAM) were chem. synthesized, and the fluorescent properties and the enzymic incorporation of the fluorophore-linked dPxTPs into DNA were examined in PCR amplification. The fluorophore-linked dPxTPs were site-specifically incorporated by PCR into DNA, opposite Ds in templates, with high selectivity. Furthermore, we found that the fluorescence of the triphosphates was partially quenched, but increased upon their incorporation into DNA. These dual fluorophore-quencher base analogs would be useful for site-specific DNA labeling and for monitoring the amplification products of target nucleic acid mols. with a specific sequence. We have demonstrated the utility of the fluorophore-linked Px substrates and the Ds-Px pairing in real-time quant. PCR for target DNA mol. detection. In the experiment, the researchers used many compounds, for example, 2,5-Dioxopyrrolidin-1-yl 3′,6′-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-5-carboxylate (cas: 92557-80-7Application of 92557-80-7).

2,5-Dioxopyrrolidin-1-yl 3′,6′-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-5-carboxylate (cas: 92557-80-7) belongs to benzofurans derivatives.Benzofuran is one of the most significant oxygen-containing heterocycles consisting of fused benzene and furan ring, which are widely presented in various naturally occurring and synthetically active compounds. Introduction of benzofurans in organic synthesis, particularly drug synthesis, involves generally the use of their metalated species as nucleophiles in addition reactions or in metal-catalysed cross-coupling reactions.Application of 92557-80-7

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Fluorescent Bisphosphonate and Carboxyphosphonate Probes: A Versatile Imaging Toolkit for Applications in Bone Biology and Biomedicine was written by Sun, Shuting;Blazewska, Katarzyna M.;Kadina, Anastasia P.;Kashemirov, Boris A.;Duan, Xuchen;Triffitt, James T.;Dunford, James E.;Russell, R. Graham G.;Ebetino, Frank H.;Roelofs, Anke J.;Coxon, Fraser P.;Lundy, Mark W.;McKenna, Charles E.. And the article was included in Bioconjugate Chemistry in 2016.Reference of 92557-80-7 This article mentions the following:

A bone imaging toolkit of 21 fluorescent probes with variable spectroscopic properties, bone mineral binding affinities, and antiprenylation activities has been created, including a novel linking strategy. The linking chem. allows attachment of a diverse selection of dyes fluorescent in the visible to near-IR range to any of the three clin. important heterocyclic bisphosphonate bone drugs (risedronate, zoledronate, and minodronate or their analogs). The resultant suite of conjugates offers multiple options to “mix and match” parent drug structure, fluorescence emission wavelength, relative bone affinity, and presence or absence of antiprenylation activity, for bone-related imaging applications. In the experiment, the researchers used many compounds, for example, 2,5-Dioxopyrrolidin-1-yl 3′,6′-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-5-carboxylate (cas: 92557-80-7Reference of 92557-80-7).

2,5-Dioxopyrrolidin-1-yl 3′,6′-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-5-carboxylate (cas: 92557-80-7) belongs to benzofurans derivatives.Benzofuran is one of the most significant oxygen-containing heterocycles consisting of fused benzene and furan ring, which are widely presented in various naturally occurring and synthetically active compounds. Benzofurans have also made significant and distinctive contributions to biology. They exhibit several biological activities that range from antioxidant, antitubercular, antiplasmodial, insecticidal.Reference of 92557-80-7

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Multicolor FRET Silica Nanoparticles by Single Wavelength Excitation was written by Wang, Lin;Tan, Weihong. And the article was included in Nano Letters in 2006.Product Details of 92557-80-7 This article mentions the following:

Fluorescent nanoparticles with multiple emission signatures by a single wavelength excitation are needed in multiplex bioanal. and mol. imaging. The authors have prepared silica nanoparticles encapsulated with three organic dyes using a modified Stoeber synthesis method. By varying the doping ratio of the three tandem dyes, fluorescence resonance energy transfer (FRET)-mediated emission signatures can be tuned to have the nanoparticles exhibit multiple colors under one single wavelength excitation. These nanoparticles are intensely fluorescent, highly photostable, uniform in size, and biocompatible. The acceptor emission of the FRET nanoparticles has generated a large Stokes shift, which implicates broad applications in biol. labeling and imaging. Mol. recognition moieties, such as biotin, can be covalently attached to the nanoparticle surface to allow for specific binding to target mols. These multicolor FRET silica nanoparticles can be used as barcoding tags for multiplexed signaling. By using these NPs, one can envision a dynamic, multicolor, colocalization methodol. to follow proteins, nucleic acids, mol. machines, and assemblies within living systems. In the experiment, the researchers used many compounds, for example, 2,5-Dioxopyrrolidin-1-yl 3′,6′-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-5-carboxylate (cas: 92557-80-7Product Details of 92557-80-7).

2,5-Dioxopyrrolidin-1-yl 3′,6′-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-5-carboxylate (cas: 92557-80-7) belongs to benzofurans derivatives. Benzofuran derivatives are one of the most important oxygen-containing heterocycles. Introduction of benzofurans in organic synthesis, particularly drug synthesis, involves generally the use of their metalated species as nucleophiles in addition reactions or in metal-catalysed cross-coupling reactions.Product Details of 92557-80-7

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Fluorescent benzothiazinone analogues efficiently and selectively label DprE1 in Mycobacteria and Actinobacteria was written by Sommer, Raphael;Neres, Joao;Piton, Jeremie;Dhar, Neeraj;van der Sar, Astrid;Mukherjee, Raju;Laroche, Thierry;Dyson, Paul J.;McKinney, John D.;Bitter, Wilbert;Makarov, Vadim;Cole, Stewart T.. And the article was included in ACS Chemical Biology in 2018.Computed Properties of C25H15NO9 This article mentions the following:

Benzothiazinones (BTZ) are highly potent bactericidal inhibitors of mycobacteria and the lead compound, BTZ043, and the optimized drug candidate, PBTZ169, have potential for the treatment of tuberculosis. Here, we exploited the tractability of the BTZ scaffold by attaching a range of fluorophores to the 2-substituent of the BTZ ring via short linkers. We show by means of fluorescence imaging that the most advanced derivative, JN108, is capable of efficiently labeling its target, the essential flavoenzyme DprE1, both in cell-free extracts and after purification as well as in growing cells of different actinobacterial species. DprE1 displays a polar localization in Mycobacterium tuberculosis, M. marinum, M. smegmatis, and Nocardia farcinica but not in Corynebacterium glutamicum. Finally, mutation of the cysteine residue in DprE1 in these species, to which BTZ covalently binds, abolishes completely the interaction with JN108, thereby highlighting the specificity of this fluorescent probe. In the experiment, the researchers used many compounds, for example, 2,5-Dioxopyrrolidin-1-yl 3′,6′-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-5-carboxylate (cas: 92557-80-7Computed Properties of C25H15NO9).

2,5-Dioxopyrrolidin-1-yl 3′,6′-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-5-carboxylate (cas: 92557-80-7) belongs to benzofurans derivatives. Many natural or synthetic compounds containing benzofuran skeletons have been found to possess remarkable activity as agrochemicals and pharmaceuticals. Substituted benzofurans find applications such as fluorescent sensors, oxidants, in drug discovery, and in another field of chemistry and agriculture.Computed Properties of C25H15NO9

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem