Tag: 179.1296

A comparison of nitrophenyl esters and lactones as substrates of cytosolic aldehyde dehydrogenase was written by Kitson, Trevor M.;Kitson, Kathryn E.. And the article was included in Biochemical Journal in 1996.Recommanded Product: 21997-23-9 This article mentions the following:

P-Nitrophenyl (PNP) acetate and propionate show a burst of p-nitrophenoxide release when their hydrolysis is catalyzed by sheep liver cytosolic aldehyde dehydrogenase. This is not seen in the presence of NAD⁺ or NADH, implying a change in rate-determining step. 6-Nitrodihydrocoumarin (6-NDC) shows no burst of absorbance in the visible region. We propose that the pK_a of the transient reporter group produced during the hydrolysis of this lactone is high (approx. 10) and that the incipient covalently linked p-nitrophenoxide moiety is protonated immediately on formation. The small burst seen in the hydrolysis of 5-nitro-2-coumaranone (5-NC) suggests that the pK_a of its reporter group is about 8.5. NADH markedly enhances the steady-state rate with the lactones. 5-NC shows a large rapid burst of color development in the presence of NADH; this implies that NADH decreases the pK_a of the reporter group to 7-7.5. In the presence of NAD^–, 5-NC and 6-NDC give an unusual neg. burst in the stopped-flow traces. We propose that, under these circumstances, acylation of the enzyme is extremely fast and that the first event seen in the stopped-flow traces is protonation of the reporter group. NAD⁺ also greatly increases the steady-state rate. With the lactones in the presence of NADH, the k_cat value (nearly 6 s^-1), a measure of the deacylation rate, is compatible with the single-site model for dehydrogenase and esterase activities. In the experiment, the researchers used many compounds, for example, 5-Nitrobenzofuran-2(3H)-one (cas: 21997-23-9Recommanded Product: 21997-23-9).

5-Nitrobenzofuran-2(3H)-one (cas: 21997-23-9) belongs to benzofurans derivatives. Many natural or synthetic compounds containing benzofuran skeletons have been found to possess remarkable activity as agrochemicals and pharmaceuticals. Introduction of benzofurans in organic synthesis, particularly drug synthesis, involves generally the use of their metalated species as nucleophiles in addition reactions or in metal-catalysed cross-coupling reactions.Recommanded Product: 21997-23-9

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

5-Nitro-1-benzofuran-2(3H)-one was written by Han, Xin-Yi;Shi, Ya-Bin;Shen, Hong;Bai, Shu-Yuan;Wang, Hai-Bo. And the article was included in Acta Crystallographica, Section E: Structure Reports Online in 2012.Safety of 5-Nitrobenzofuran-2(3H)-one This article mentions the following:

In the crystal structure of the title compound, C₈H₅NO₄, essentially planar mols. [largest deviation from the least-squares plane = 0.030(2) Å] form stacks along the a-axis direction. Intercentroid separations between overlapping benzene rings within the stack are 3.6594(12) Å and 3.8131(12) Å. Mols. from neighboring stacks are linked by weak C-H···O H bonds into inversion dimers. Crystallog. data and at. coordinates are given. In the experiment, the researchers used many compounds, for example, 5-Nitrobenzofuran-2(3H)-one (cas: 21997-23-9Safety of 5-Nitrobenzofuran-2(3H)-one).

5-Nitrobenzofuran-2(3H)-one (cas: 21997-23-9) belongs to benzofurans derivatives. Benzofuran is a core structural unit found in many naturally occurring compounds with multidirectional biological activities. In nature, benzofurans have occupied an important role among the plant phenols & several pharmacologically active compounds.Safety of 5-Nitrobenzofuran-2(3H)-one

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem