Predictive Endocrine Testing in the 21st Century Using in Vitro Assays of Estrogen Receptor Signaling Responses was written by Rotroff, Daniel M.;Martin, Matt T.;Dix, David J.;Filer, Dayne L.;Houck, Keith A.;Knudsen, Thomas B.;Sipes, Nisha S.;Reif, David M.;Xia, Menghang;Huang, Ruili;Judson, Richard S.. And the article was included in Environmental Science & Technology in 2014.Quality Control of 2,2-Dimethyl-2,3-dihydrobenzofuran-7-ol This article mentions the following:
Thousands of environmental chems. are subject to regulatory review for their potential to be endocrine disruptors (ED). In vitro high-throughput screening (HTS) assays have emerged as a potential tool for prioritizing chems. for ED-related whole-animal tests. In this study, 1814 chems. including pesticide active and inert ingredients, industrial chems., food additives, and pharmaceuticals were evaluated in a panel of 13 in vitro HTS assays. The panel of in vitro assays interrogated multiple end points related to estrogen receptor (ER) signaling, namely binding, agonist, antagonist, and cell growth responses. The results from the in vitro assays were used to create an ER Interaction Score. For 36 reference chems., an ER Interaction Score >0 showed 100% sensitivity and 87.5% specificity for classifying potential ER activity. The magnitude of the ER Interaction Score was significantly related to the potency classification of the reference chems. ERα/ERβ selectivity was also evaluated, but relatively few chems. showed significant selectivity for a specific isoform. When applied to a broader set of chems. with in vivo uterotrophic data, the ER Interaction Scores showed 91% sensitivity and 65% specificity. Overall, this study provides a novel method for combining in vitro concentration response data from multiple assays and, when applied to a large set of ER data, accurately predicted estrogenic responses and demonstrated its utility for chem. prioritization. In the experiment, the researchers used many compounds, for example, 2,2-Dimethyl-2,3-dihydrobenzofuran-7-ol (cas: 1563-38-8Quality Control of 2,2-Dimethyl-2,3-dihydrobenzofuran-7-ol).
2,2-Dimethyl-2,3-dihydrobenzofuran-7-ol (cas: 1563-38-8) belongs to benzofurans derivatives. Benzofuran derivatives have shown many biological activities, including antifungal and antimicrobial properties, and acting as antagonists of H3 receptors and angiotensin II. In nature, benzofurans have occupied an important role among the plant phenols & several pharmacologically active compounds.Quality Control of 2,2-Dimethyl-2,3-dihydrobenzofuran-7-ol
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem