Effect and mechanism of wedelolactone as antioxidant-coumestan on ·OH-treated mesenchymal stem cells was written by Li, Xican;Wang, Tingting;Liu, Jingjing;Liu, Yulong;Zhang, Jun;Lin, Jian;Zhao, Zhongxiang;Chen, Dongfeng. And the article was included in Arabian Journal of Chemistry in 2020.Related Products of 524-12-9 The following contents are mentioned in the article:
The antioxidant properties of coumestans have been investigated previously using inappropriate methods, which has resulted in misleading and inaccurate mechanisms being reported to account for their antioxidant behavior. In this study, the phenolic coumestan wedelolactone increased the viability of ·OH-treated mesenchymal stem cells (MSCs) in a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl assay. Mechanistic anal. in vitro suggested that wedelolactone could scavenge various radicals, including ·OH, ·O–2, 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) and 1,1-diphenyl-2-picrylhydrazyl (DPPH·) radicals, as well as reducing Cu2+ and chelating Fe2+. Configuration anal. based on a ball and stick model indicated that the 3′,4′-catechol moiety of wedelolactone was acting as an Fe2+-chelating site. The main product of the reaction between wedelolactone and DPPH· was analyzed by ultra-performance liquid chromatog. electrospray ionization quadrupole time-of-flight tandem mass spectrometry, which gave a mass ion with an m/z value of 709. This peak yielded several fragments with m/z values of 663, 543, 501 and 483. Quant. evaluation revealed that the antioxidant activity of wedelolactone was 1.62 times higher than that of Trolox. Based on these results, we concluded that (i) wedelolactone can efficiently protect MSCs against ·OH-induced damage and that this protective effect provides preliminary evidence for the application of wedelolactone in transplantation of MSCs (especially for osteoporosis); (ii) the main antioxidant mechanism of wedelolactone involves direct radical-scavenging via a single electron transfer (SET) → radical adduct formation (RAF) pathway, whereas the Fe2+-chelating activity of wedelolactone represents a minor pathway; and (iii) the direct radical-scavenging and Fe2+-chelating pathways can both be attributed to the catechol moiety rather than the coumestan skeleton. This study involved multiple reactions and reactants, such as 1,8,9-Trihydroxy-3-methoxy-6H-benzofuro[3,2-c]chromen-6-one (cas: 524-12-9Related Products of 524-12-9).
1,8,9-Trihydroxy-3-methoxy-6H-benzofuro[3,2-c]chromen-6-one (cas: 524-12-9) belongs to benzofurans derivatives. Benzofuran derivatives have shown many biological activities, including antifungal and antimicrobial properties, and acting as antagonists of H3 receptors and angiotensin II. In nature, benzofurans have occupied an important role among the plant phenols & several pharmacologically active compounds.Related Products of 524-12-9
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem