Elicitor mediated enhancement of wedelolactone in cell suspension culture of Eclipta alba (L.) Hassk was written by Salma, Umme;Kundu, Suprabuddha;Ali, Nasim Md.;Mandal, Nirmal. And the article was included in Plant Cell, Tissue and Organ Culture in 2018.SDS of cas: 524-12-9 The following contents are mentioned in the article:
The present research focused on enhancing the production of wedelolactone through cell suspension culture (CSC) in Eclipta alba (L.) Hassk. With an aim of attaining a sustainable CSC, various plant growth regulators, elicitors and agitation speed were examined Nodal segments of in vitro propagated plantlets induced the maximum percentage (93.47 ± 0.61%) of callus inoculated on Murashige and Skoog (MS) medium fortified with picloram (2 mg L-1). The growth kinetics of CSC exhibited a sigmoid pattern with a lag phase (0-6 days), a log phase (6-18 days), a stationary phase (18-24 days) and then death phase thereafter. The highest biomass accumulation in CSC with 7.09 ± 0.06 g 50 mL-1 fresh weight, 1.52 ± 0.02 g 50 mL-1 dry cell weight, 1.34 ± 0.01 × 106 cell mL-1 total cell count and 57.00 ± 0.58% packed cell volume was obtained in the liquid MS medium supplemented with 1.5 mg L-1 picloram plus 0.5 mg L-1 kinetin at 120 rpm. High performance thin layer chromatog. confirmed that yeast extract (biotic elicitor) at 150 mg L-1 accumulated more CSC biomass with 1.22-fold increase in wedelolactone (288.97 ± 1.94μg g-1 dry weight) content in comparison to the non-elicited CSC (237.78 ± 0.04μg g-1 dry weight) after 120 h of incubation. Contrastingly, Me jasmonate (abiotic elicitor) did not alter the biomass but increased the wedelolactone content (259.32 ± 1.06μg g-1 dry weight) to an extent of 1.09-fold at 100μM. Complete plantlet regeneration from CSC was possible on MS medium containing N6-benzyladenine (0.75 mg L-1) and abscisic acid (0.5 mg L-1). Thus, the establishment of protocol for CSC constitutes the bases for future biotechnol. improvement studies in this crop. This study involved multiple reactions and reactants, such as 1,8,9-Trihydroxy-3-methoxy-6H-benzofuro[3,2-c]chromen-6-one (cas: 524-12-9SDS of cas: 524-12-9).
1,8,9-Trihydroxy-3-methoxy-6H-benzofuro[3,2-c]chromen-6-one (cas: 524-12-9) belongs to benzofurans derivatives. Benzofuran derivatives are one of the most important oxygen-containing heterocycles. Benzofurans have also made significant and distinctive contributions to biology. They exhibit several biological activities that range from antioxidant, antitubercular, antiplasmodial, insecticidal.SDS of cas: 524-12-9
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem