Application In Synthesis of (R)-Ethyl 4-chloro-3-hydroxybutanoate. Aromatic heterocyclic compounds can also be classified according to the number of heteroatoms contained in the heterocycle: single heteroatom, two heteroatoms, three heteroatoms and four heteroatoms. Compound: (R)-Ethyl 4-chloro-3-hydroxybutanoate, is researched, Molecular C6H11ClO3, CAS is 90866-33-4, about Cloning, expression, and characterization of an (R)-specific alcohol dehydrogenase from Lactobacillus kefir. Author is Weckbecker, Andrea; Hummel, Werner.
Lactobacillus kefir DSM 20587 produces an (R)-specific NADP-dependent alc. dehydrogenase (ADH) with a broad substrate specificity. The gene of this ADH was isolated and the complete nucleotide sequence determined The adh gene comprises 759 bp and encodes a protein of 252 amino acids with a calculated mol. weight of 26 781 Da. The deduced amino acid sequence indicated a high degree of similarity to short-chain dehydrogenases. After cloning and expression in Escherichia coli the enzyme was purified and characterized. For the reduction of acetophenone the specific activity of the homogeneous recombinant ADH was 558 U mg-1. The enzyme shows its maximum activity at 50°C while the pH optimum was at pH 7.0. In order to demonstrate its preparative application, purified ADH was used for the stereoselective reduction of several aliphatic and aromatic ketones as well as β-keto esters. Glucose dehydrogenase was added for the regeneration of NADPH. All prochiral ketones were stereoselectively reduced to the corresponding alcs. with >99% ee and in the case of diketones >99% de.
Different reactions of this compound((R)-Ethyl 4-chloro-3-hydroxybutanoate)Application In Synthesis of (R)-Ethyl 4-chloro-3-hydroxybutanoate require different conditions, so the reaction conditions are very important.
Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem