Formula: C18H20N2O12. Aromatic heterocyclic compounds can also be classified according to the number of heteroatoms contained in the heterocycle: single heteroatom, two heteroatoms, three heteroatoms and four heteroatoms. Compound: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, is researched, Molecular C18H20N2O12, CAS is 70539-42-3, about Thermostabilization of Bacillus amyloliquefaciens α-amylase by chemical crosslinking. Author is Habibi, Azadeh Ebrahim; Khajeh, Khosro; Naderi-Manesh, Hossein; Ranjbar, Bijan; Nemat-Gorgani, Mohsen.
Chem. crosslinking of a mesophilic α-amylase from Bacillus amyloliquefaciens (BAA) was carried out. Intramol. crosslinks between Lys residues upon treatment of the enzyme with ethylene glycol bis(succinic acid N-hydroxy succinimide ester) resulted in enhancement of thermostability as indicated by irreversible thermoinactivation experiments Enhancement of thermostability coincided with a dramatic protection against aggregation, combined with a decrease in surface hydrophobicity. Deamidation, another important mechanism of irreversible thermoinactivation, was also diminished upon modification. While no significant changes in the kinetic parameters were evident, rigidification of the protein structure was suggested by CD and fluorescence studies.
The article 《Thermostabilization of Bacillus amyloliquefaciens α-amylase by chemical crosslinking》 also mentions many details about this compound(70539-42-3)Formula: C18H20N2O12, you can pay attention to it, because details determine success or failure
Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem