COA of Formula: C18H20N2O12. Aromatic compounds can be divided into two categories: single heterocycles and fused heterocycles. Compound: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, is researched, Molecular C18H20N2O12, CAS is 70539-42-3, about Covalent affinity labeling, detergent solubilization, and fluid-phase characterization of the rabbit neutrophil formyl peptide chemotaxis receptor. Author is Marasco, Wayne A.; Becker, Kathleen M.; Feltner, Douglas E.; Brown, C. Susan; Ward, Peter A.; Nairn, Roderick.
The formyl peptide chemotaxis receptor of rabbit neutrophils and purified rabbit neutrophil plasma membranes was identified by several affinity labeling techniques: (1) covalent affinity crosslinking of N-formyl-Nle-Leu-Phe-Nle-125I-Tyr-Lys (125I-hexapeptide) to the membrane-bound receptor with either di-Me suberimidate or ethylene glycol bis(succinimidyl succinate) and (2) photoactivation of N-formyl-Nle-Leu-Phe-Nle-125I-Tyr-Lys-Nε-6-[(4-azido-2-nitrophenyl)amino]hexanoate (125I-PAL). These techniques specifically identify the receptor as a polypeptide that migrates as a broad band on SDS-polyacrylamide electrophoresis, with a mol. weight (Mr) 50,000-65,000. The receptor was solubilized in active form from rabbit neutrophil membranes by using the detergents 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) and digitonin, and from whole cells with CHAPS. Chemotaxis receptor activity was measured by the ability of the solubilized membrane material to bind 125I-hexapeptide or N-formyl-Met-Leu-[3H]Phe with gel filtration or rapid filtration through poly(ethylenimine) (PEI)-treated filters as assay systems. 125I-PAL was specifically crosslinked to the same mol. weight material in the CHAPS and digitonin solubilized extract, but no specific labeling of the receptor was seen when membranes were extracted with Nonidet P-40 and Triton X-100. Therefore, although a large number of detergents are able to solubilize the receptor, apparently some detergents release the receptor in an inactive form. The ligand binding characteristics of formyl-Met-Leu-[3H]Phe to the CHAPS-solubilized receptor shared properties with the membrane-bound formyl peptide receptor, both of which showed curvilinear, concave-upward Scatchard plots. Computer curve fitting with the program NONLIN and statistical analyses of the binding data indicated that for both the membrane-bound and solubilized receptors a 2-saturable sites model fitted the data significantly better than did a 1-saturable site model. The characteristics of the 2-saturable sites model for the soluble receptor were: a high-affinity site with a KD value of 1.25 nM and a low-affinity site with a KD value of 19.77 nM. A total of 35% of the 2 sites detected was of the higher affinity. A Hill coefficient of 0.61 was observed
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Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem