Electric Literature of 97148-39-5, Catalysts allow a reaction to proceed via a pathway that has a lower activation energy than the uncatalyzed reaction. 97148-39-5, Name is Ammonium (Z)-2-(furan-2-yl)-2-(methoxyimino)acetate, SMILES is O=C([O-])/C(C1=CC=CO1)=NOC.[NH4+], belongs to benzofurans compound. In a article, author is Santos, Gustavo de Almeida, introduce new discover of the category.
Directed Evolution of P450 BM3 towards Functionalization of Aromatic O-Heterocycles
The O-heterocycles, benzo-1,4-dioxane, phthalan, isochroman, 2,3-dihydrobenzofuran, benzofuran, and dibenzofuran are important building blocks with considerable medical application for the production of pharmaceuticals. Cytochrome P450 monooxygenase (P450) Bacillus megaterium 3 (BM3) wild type (WT) from Bacillus megaterium has low to no conversion of the six O-heterocycles. Screening of in-house libraries for active variants yielded P450 BM3 CM1 (R255P/P329H), which was subjected to directed evolution and site saturation mutagenesis of four positions. The latter led to the identification of position R255, which when introduced in the P450 BM3 WT, outperformed all other variants. The initial oxidation rate of nicotinamide adenine dinucleotide phosphate (NADPH) consumption increased approximate to 140-fold (WT: 8.3 +/- 1.3 min(-1); R255L: 1168 +/- 163 min(-1)), total turnover number (TTN) increased approximate to 21-fold (WT: 40 +/- 3; R255L: 860 +/- 15), and coupling efficiency, approximate to 2.9-fold (WT: 8.8 +/- 0.1%; R255L: 25.7 +/- 1.0%). Computational analysis showed that substitution R255L (distant from the heme-cofactor) does not have the salt bridge formed with D217 in WT, which introduces flexibility into the I-helix and leads to a heme rearrangement allowing for efficient hydroxylation.
Electric Literature of 97148-39-5, Enzymes are biological catalysts that produce large increases in reaction rates and tend to be specific for certain reactants and products. I hope my blog about 97148-39-5 is helpful to your research.
Reference:
Benzofuran – Wikipedia,
,Benzofuran | C8H6O – PubChem