Category: 3326-34-9

Conjugation of amino-bioactive glasses with 5-aminofluorescein as probe molecule for the development of pH sensitive stimuli-responsive biomaterials was written by Aina, Valentina;Malavasi, Gianluca;Magistris, Claudio;Cerrato, Giuseppina;Martra, Gianmario;Viscardi, Guido;Menabue, Ledi;Lusvardi, Gigliola. And the article was included in Journal of Materials Science: Materials in Medicine in 2014.Reference of 3326-34-9 This article mentions the following:

Bioceramics, such as silica-based glasses, are widely used in bone and teeth restoration. Nowadays, the association between nanotechnol. and pharmacol. is one of the most promising research fields in cancer therapy. The advanced processing methods and new chem. strategies allow the incorporation of drugs within them or on their functionalized surfaces. Bioceramics can act as local drug delivery systems to treat bone and teeth diseases. The present paper reports data related to the development of a pH-stimuli responsive bioactive glass. The glass conjugation with 5-aminofluorescein (5-AF), through a pH-sensitive organic spacer, allows to produce a pH-responsive bioactive biomaterial: when it is exposed to specific pH changes, it can favor the release of 5-AF directly at the target site. 5-AF has been chosen as a simple, low cost, non toxic model to simulate doxorubicin, an anticancer drug. As doxorubicin, 5-AF contains an amino group in its structure to form an amide bond with the carboxylic functionalities of the glass. Raman spectroscopy and thermal anal. confirm the glass conjugation of 5-AF by an amide bond; the amount of 5-AF loaded was very high (≈65 and 44%). The release tests at two different pH (4.2 and 7.4) show that the amount of released 5-AF is higher at acid pH with respect to physiol. one. This preliminary datum evidenced that a pH-sensitive drug delivery system has been developed. The low amount of 5-AF released (<1% of the total 5-AF) is due to the very low solubility of 5-AF in aqueous medium. This disadvantage, may be overcome in a dynamic environment (physiol. conditions), where it is possible to obtain a drug release system ensuring an effective therapeutic dose for long times and, at the same time, avoiding the drug toxicity. In the experiment, the researchers used many compounds, for example, 5-Amino-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 3326-34-9Reference of 3326-34-9).

5-Amino-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 3326-34-9) belongs to benzofurans derivatives. Many natural or synthetic compounds containing benzofuran skeletons have been found to possess remarkable activity as agrochemicals and pharmaceuticals. Benzofurans have also made significant and distinctive contributions to biology. They exhibit several biological activities that range from antiviral, antimicrobial, antitumor, anti-inflammatory.Reference of 3326-34-9

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Dextran-based fluorescent nanoprobes for sentinel lymph node mapping was written by Dai, Tingting;Zhou, Shuyan;Yin, Chuyang;Li, Shengli;Cao, Weigang;Liu, Wei;Sun, Kang;Dou, Hongjing;Cao, Yilin;Zhou, Guangdong. And the article was included in Biomaterials in 2014.Formula: C20H13NO5 This article mentions the following:

Biopsy of sentinel lymph node (SLN) has become a common practice to predict whether tumor metastasis has occurred, so proper SLN positioning tracers are highly required. Due to many drawbacks of SLN tracers currently used, developing ideal, biosafe SLN imaging agents is always an urgent issue. The current study designed a novel fluorescent nanoprobe for accurate SLN mapping. Dextran-based nanogel (DNG) was prepared through a highly efficient self-assembly assisted approach and serves as a multi-functional platform for conjugating wide spectra emitting fluorescent agents. The newly fabricated fluorescent DNG (FDNG) could be designed with optimum size and stable fluorescent intensity for specific SLN imaging. Furthermore, a long-term dynamic course in vivo (from 1 min to 72 h) revealed the satisfactory specificity, sensitivity, and stability for SLN mapping. Most importantly, both in vitro and in vivo evaluations indicated that FDNG had fine biosafety and biocompatibility with lymphatic endothelial cells. All these results supported that FDNG could be used as highly efficient mol. imaging probes for specific, sensitive, stable, non-invasive, and safe SLN mapping, which provides efficient and accurate location for SLN biopsy and thus predicts tumor metastasis as well as directs therapies. Besides, our recent studies further demonstrated that DNG could also serve as a specific and controllable drug carrier, indicating a potential application for specific therapies of various lymph-associated diseases. In the experiment, the researchers used many compounds, for example, 5-Amino-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 3326-34-9Formula: C20H13NO5).

5-Amino-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 3326-34-9) belongs to benzofurans derivatives. Benzofurans are compounds with a planar structure having 10 pi electrons that include the lone pair on oxygen atom, which makes it more susceptible to electrophilic attack. As benzofurans are prone to undergo ring opening of the heterocycle, examples of reduction of this type of aromatics by using dissolving metals are rather scarce.Formula: C20H13NO5

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Multi-small molecule conjugations as new targeted delivery carriers for tumor therapy was written by Shan, Lingling;Liu, Ming;Wu, Chao;Zhao, Liang;Li, Siwen;Xu, Lisheng;Cao, Wengen;Gao, Guizhen;Gu, Yueqing. And the article was included in International Journal of Nanomedicine in 2015.Application of 3326-34-9 This article mentions the following:

In response to the challenges of cancer chemotherapeutics, including poor physicochem. properties, low tumor targeting ability, and harmful side effects, we developed a new tumor-targeted multi-small mol. drug delivery platform. Using paclitaxel (PTX) as a model therapeutic, we prepared two prodrugs, ie, folic acid-fluorescein-5(6)-isothiocyanate-arginine-paclitaxel (FA-FITC-Arg-PTX) and folic acid-5-aminofluorescein-glutamic-paclitaxel (FA-5AF-Glu-PTX), composed of folic acid (FA, target), amino acids (Arg or Glu, linker), and fluorescent dye (fluorescein in vitro or near-IR fluorescent dye in vivo) in order to better understand the mechanism of PTX prodrug targeting. In vitro and acute toxicity studies demonstrated the low toxicity of the prodrug formulations compared with the free drug. In vitro and in vivo studies indicated that folate receptor-mediated uptake of PTX-conjugated multi-small mol. carriers induced high antitumor activity. Notably, compared with free PTX and with PTX-loaded macromol. carriers from our previous study, this multi-small mol.-conjugated strategy improved the water solubility, loading rate, targeting ability, antitumor activity, and toxicity profile of PTX. These results support the use of multi-small mols. as tumor-targeting drug delivery systems. In the experiment, the researchers used many compounds, for example, 5-Amino-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 3326-34-9Application of 3326-34-9).

5-Amino-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 3326-34-9) belongs to benzofurans derivatives. Benzofuran is a core structural unit found in many naturally occurring compounds with multidirectional biological activities. They are also prone to polymerisation in the presence of concentrated mineral acids and Lewis acids.Application of 3326-34-9

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Identification of Hydrogen Peroxide-Secreting Cells by Cytocompatible Coating with a Hydrogel Membrane was written by Liu, Yang;Sakai, Shinji;Kawa, Shogo;Taya, Masahito. And the article was included in Analytical Chemistry (Washington, DC, United States) in 2014.Reference of 3326-34-9 This article mentions the following:

A method for identifying each cell secreting reactive oxygen species (ROS) is highly desirable to advance the understanding of the physiol. and pathol. processes attributed to extracellular ROS. Here, the authors first report a method for realizing this. The individual cells secreting hydrogen peroxide (H₂O₂), a common ROS, could be coated by a hydrogel membrane through a horseradish peroxidase-catalyzed reaction consuming H₂O₂ secreted from the cells themselves. This hydrogel membrane coating is cytocompatible. In addition, the hydrogel membrane made from an alginate derivative could be removed on demand without causing damage to the enclosed cells. These results demonstrated the feasibility of the proposed method to be an effective tool in cellular ROS studies. In the experiment, the researchers used many compounds, for example, 5-Amino-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 3326-34-9Reference of 3326-34-9).

5-Amino-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 3326-34-9) belongs to benzofurans derivatives. Benzofurans are compounds with a planar structure having 10 pi electrons that include the lone pair on oxygen atom, which makes it more susceptible to electrophilic attack. Benzofurans have also made significant and distinctive contributions to biology. They exhibit several biological activities that range from antioxidant, antitubercular, antiplasmodial, insecticidal.Reference of 3326-34-9

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Toxicokinetics of mono-(2-ethylhexyl) phthalate with low-dose exposure applying fluorescence tracing technique was written by Yuan, Yi-Zhen;Ye, Chong;Sun, Jia-Hui;Hu, Meng-Yuan;Huo, Shao-Jie;Zhu, Yu-Ting;Xiang, Su-Yun;Yu, Shu-Qin. And the article was included in Toxicology and Applied Pharmacology in 2022.Name: 5-Amino-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one This article mentions the following:

Di(2-ethylhexyl) phthalate (DEHP) belongs to environmental endocrine disrupting chems. (EEDCs) and can be rapidly hydrolyzed into the ultimate toxicant mono-2-ethylhexyl phthalate (MEHP). In this study, we used 5-aminofluorescein modified MEHP (MEHP-AF) as a fluorescence tracer to explore the toxicokinetics, including toxicokinetic parameters, absorption and transport across the intestinal mucosal barrier, distribution and pathol. changes of organs. While the dose was as lower than 10 mg/kg by intragastric administration, the toxicokinetic parameters obtained by fluorescence microplate method were similar to those with the literatures by chromatog. MEHP-AF can be rapidly absorbed through the intestinal mucosal barrier in rats. In situ organ distribution in mice showed that MEHP-AF was mainly concentrated in the liver, kidney and testis. Our results suggested that the fluorescence tracing technique had the advantages with easy processing, less time-consuming, higher sensitivity for the quant. determination, In addition, this technol. also avoids the interference of exogenous or endogenous DEHP and MEHP in the exptl. system. It also can be utilized to the visualization detection of MEHP in situ localization in the absorption organ and the toxic target organ. The results show that this may be a more feasible MEHP toxicol. research method. In the experiment, the researchers used many compounds, for example, 5-Amino-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 3326-34-9Name: 5-Amino-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one).

5-Amino-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 3326-34-9) belongs to benzofurans derivatives. Benzofurans are only weakly aromatic in nature and they are cleaved by many oxidative and reductive conditions. Benzofurans have also made significant and distinctive contributions to biology. They exhibit several biological activities that range from antiviral, antimicrobial, antitumor, anti-inflammatory.Name: 5-Amino-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Non-toxic fluorescent alanine-fluorescein probe with green emission for dual colorimetric/fluorimetric sensing was written by Oliveira, Elisabete;Lorenzo, Julia;Cid, Antonio;Capelo, Jose Luis;Lodeiro, Carlos. And the article was included in Journal of Photochemistry and Photobiology, A: Chemistry in 2013.COA of Formula: C20H13NO5 This article mentions the following:

Two new fluorescent bio-inspired dyes 1 and 2 containing a fluorescein unit linked to a protected with a Boc group (N-tert-butyloxycarbonyl), and unprotected alanine residue were synthesized and characterized. The acid-base behavior was studied in water/ethanol (60/40) and at pH = 8 a strong emission fluorescence, followed by a color change from colorless to yellow was observed Photophys. studies in water HEPES buffered solution toward alk., alk. earth, transition, and post- transition metal ions were performed. Compounds 1 and 2 revealed to be very sensitive to Hg²⁺ with colorimetric changes from yellow to pink in the presence of HEPES buffer. Moreover, compound 1 show a color change from yellow to orange in the presence of the neurotransmitter mol. dopamine. Compound 2 also reveals to be sensitive to Cu²⁺ and Ni²⁺, being able to detected and quantified in HEPES buffer ca. 0.37 ppm and ca. 1.50 ppm of Cu²⁺, resp. Very small silver nanoparticles coated with compound 2 of ca. 3.68 ± 0.93 nm were successfully synthesized and characterized. Toxicol. studies with both compounds were also carried out. In the experiment, the researchers used many compounds, for example, 5-Amino-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 3326-34-9COA of Formula: C20H13NO5).

5-Amino-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 3326-34-9) belongs to benzofurans derivatives. Benzofuran is the “”parent”” of many related compounds with more complex structures. For example, psoralen is a benzofuran derivative that occurs in several plants. As benzofurans are prone to undergo ring opening of the heterocycle, examples of reduction of this type of aromatics by using dissolving metals are rather scarce.COA of Formula: C20H13NO5

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Tritium labelling of a cholesterol amphiphile designed for cell membrane anchoring of proteins was written by Schaefer, Balazs;Orban, Erika;Kele, Zoltan;Toemboely, Csaba. And the article was included in Journal of Labelled Compounds and Radiopharmaceuticals in 2015.Quality Control of 5-Amino-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one This article mentions the following:

Cell membrane association of proteins can be achieved by the addition of lipid moieties to the polypeptide chain, and such lipid-modified proteins have important biol. functions. A class of cell surface proteins contains a complex glycosylphosphatidylinositol (GPI) glycolipid at the C-terminus, and they are accumulated in cholesterol-rich membrane microdomains, i.e., lipid rafts. Semisynthetic lipoproteins prepared from recombinant proteins and designed lipids are valuable probes and model systems of the membrane-associated proteins. Because GPI-anchored proteins can be reinserted into the cell membrane with the retention of the biol. function, they are appropriate candidates for preparing models via reduction of the structural complexity. A synthetic headgroup was added to the 3β-hydroxyl group of cholesterol, an essential lipid component of rafts, and the resulting cholesterol derivative was used as a simplified GPI mimetic. In order to quantitate the membrane integrated GPI mimetic after the exogenous addition to live cells, a tritium labeled cholesterol anchor was prepared The radioactive label was introduced into the headgroup, and the radiolabeled GPI mimetic anchor was obtained with a specific activity of 1.37 TBq/mmol. The headgroup labeled cholesterol derivative was applied to demonstrate the sensitive detection of the cell membrane association of the anchor under in vivo conditions. In the experiment, the researchers used many compounds, for example, 5-Amino-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 3326-34-9Quality Control of 5-Amino-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one).

5-Amino-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 3326-34-9) belongs to benzofurans derivatives.Benzofuran is one of the most significant oxygen-containing heterocycles consisting of fused benzene and furan ring, which are widely presented in various naturally occurring and synthetically active compounds. They are also prone to polymerisation in the presence of concentrated mineral acids and Lewis acids.Quality Control of 5-Amino-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Chemical Labeling and Affinity Capture of Inosine-Containing RNAs Using Acrylamidofluorescein was written by Knutson, Steve D.;Ayele, Tewoderos M.;Heemstra, Jennifer M.. And the article was included in Bioconjugate Chemistry in 2018.COA of Formula: C20H13NO5 This article mentions the following:

Adenosine-to-inosine (A-to-I) RNA editing is a widespread and conserved posttranscriptional modification, producing significant changes in cellular function and behavior. Accurately identifying, detecting, and quantifying these sites in the transcriptome is necessary to improve the understanding of editing dynamics, its broader biol. roles, and connections with diseases. Chem. labeling of edited bases coupled with affinity enrichment has enabled improved characterization of several forms of RNA editing. However, there are no approaches currently available for pull-down of inosines. To address this need, the authors explore acrylamide as a labeling motif and report here an acrylamidofluorescein reagent that reacts with inosine and enables enrichment of inosine-containing RNA transcripts. This method provides improved sensitivity in the detection and identification of inosines toward a more comprehensive transcriptome-wide anal. of A-to-I editing. Acrylamide derivatization is also highly generalizable, providing potential for the labeling of inosine with a wide variety of probes and affinity handles. In the experiment, the researchers used many compounds, for example, 5-Amino-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 3326-34-9COA of Formula: C20H13NO5).

5-Amino-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 3326-34-9) belongs to benzofurans derivatives. Benzofurans are compounds with a planar structure having 10 pi electrons that include the lone pair on oxygen atom, which makes it more susceptible to electrophilic attack. Benzofurans are stable towards alkali and readily polymerize on treatment with sulfuric acid, due to which they are useful for the preparation of low cost chemically relatively inert resins.COA of Formula: C20H13NO5

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Microfluidic reactors based on rechargeable catalytic porous supports: Heterogeneous enzymatic catalysis via reversible host-guest interactions was written by Sanz de Leon, Alberto;Vargas-Alfredo, Nelson;Gallardo, Alberto;Fernandez-Mayoralas, Alfonso;Bastida, Agatha;Munoz-Bonilla, Alexandra;Rodriguez-Hernandez, Juan. And the article was included in ACS Applied Materials & Interfaces in 2017.HPLC of Formula: 3326-34-9 This article mentions the following:

We report on the fabrication of a microfluidic device in which the reservoir contains a porous surface with enzymic catalytic activity provided by the reversible immobilization of horseradish peroxidase onto micrometer size pores. The porous functional reservoir was obtained by the Breath Figures approach by casting in a moist environment a solution containing a mixture of high mol. weight polystyrene (HPS) and a poly(styrene-co-cyclodextrin based styrene) (P(S-co-SCD)) statistical copolymer. The pores enriched in CD were employed to immobilize horseradish peroxidase (previously modified with adamantane) by host-guest interactions (HRP-Ada). These surfaces exhibit catalytic activity that remains stable during several reaction cycles. Moreover, the porous platforms could be recovered by using free water-soluble β-CD with detergents. An excess of β-CD/TritonX100 in solution disrupts the interactions between HRP-Ada and the CD-modified substrate thus allowing us to recover the employed enzyme and reuse the platform. In the experiment, the researchers used many compounds, for example, 5-Amino-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 3326-34-9HPLC of Formula: 3326-34-9).

5-Amino-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 3326-34-9) belongs to benzofurans derivatives. Benzofuran is a core structural unit found in many naturally occurring compounds with multidirectional biological activities. They are also prone to polymerisation in the presence of concentrated mineral acids and Lewis acids.HPLC of Formula: 3326-34-9

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem