Identification of Hydrogen Peroxide-Secreting Cells by Cytocompatible Coating with a Hydrogel Membrane was written by Liu, Yang;Sakai, Shinji;Kawa, Shogo;Taya, Masahito. And the article was included in Analytical Chemistry (Washington, DC, United States) in 2014.Reference of 3326-34-9 This article mentions the following:
A method for identifying each cell secreting reactive oxygen species (ROS) is highly desirable to advance the understanding of the physiol. and pathol. processes attributed to extracellular ROS. Here, the authors first report a method for realizing this. The individual cells secreting hydrogen peroxide (H2O2), a common ROS, could be coated by a hydrogel membrane through a horseradish peroxidase-catalyzed reaction consuming H2O2 secreted from the cells themselves. This hydrogel membrane coating is cytocompatible. In addition, the hydrogel membrane made from an alginate derivative could be removed on demand without causing damage to the enclosed cells. These results demonstrated the feasibility of the proposed method to be an effective tool in cellular ROS studies. In the experiment, the researchers used many compounds, for example, 5-Amino-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 3326-34-9Reference of 3326-34-9).
5-Amino-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 3326-34-9) belongs to benzofurans derivatives. Benzofurans are compounds with a planar structure having 10 pi electrons that include the lone pair on oxygen atom, which makes it more susceptible to electrophilic attack. Benzofurans have also made significant and distinctive contributions to biology. They exhibit several biological activities that range from antioxidant, antitubercular, antiplasmodial, insecticidal.Reference of 3326-34-9
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem