Chemistry is an experimental science, and the best way to enjoy it and learn about it is performing experiments. Product Details of 1563-38-8. Introducing a new discovery about 1563-38-8, Name is 2,2-Dimethyl-2,3-dihydrobenzofuran-7-ol
6-Chloro-2-benzoxazolinone (CDHB) is a precursor of herbicide, insecticide, and fungicide synthesis and has a broad spectrum of biological activity. Pigmentiphaga sp. strain DL-8 can transform CDHB into 2-amino-5-chlorophenol (2A5CP), which it then utilizes as a carbon source for growth. The CDHB hydrolase (CbaA) was purified from strain DL-8, which can also hydrolyze 2-benzoxazolinone (BOA), 5-chloro-2-BOA, and benzamide. The specific activity of purified CbaA was 5,900 U . mg protein-1 for CDHB, with Km and kcat values of 0.29mMand 8,500 s-1, respectively. The optimal pH for purified CbaA was 9.0, the highest activity was observed at 55C, and the inactive metal-free enzyme could be reactivated by Mg2+, Ni2+, Ca2+, or Zn2+. Based on the results obtained for the CbaA peptide mass fingerprinting and draft genome sequence of strain DL-8, cbaA (encoding 339 amino acids) was cloned and expressed in Escherichia coli BL21(DE3). CbaA shared 18 to 21% identity with some metal-dependent hydrolases of the PF01499 family and contained the signature metal-binding motif Q127XXXQ131XD133XXXH137. The conserved amino acid residues His288 and Glu301 served as the proton donor and acceptor. E. coli BL21(DE3-pET-cbaA) resting cells could transform 0.2mMCDHB into 2A5CP. The mutant strain DL-8DeltacbaA lost the ability to degrade CDHB but retained the ability to degrade 2A5CP, consistent with strain DL-8. These results indicated that cbaA was the key gene responsible for CDHB degradation by strain DL-8.
Note that a catalyst decreases the activation energy for both the forward and the reverse reactions and hence accelerates both the forward and the reverse reactions.Product Details of 1563-38-8, you can also check out more blogs about1563-38-8
Reference：
Benzofuran – Wikipedia,
Benzofuran | C8H2356O – PubChem