Titration of lysine residues on adenine nucleotide translocase by fluorescamine induces permeability transition was written by Buelna-Chontal, Mabel;Pavon, Natalia;Correa, Francisco;Hernandez-Esquivel, Luz;Chavez, Edmundo. And the article was included in Cell Biology International in 2014.HPLC of Formula: 38183-12-9 This article mentions the following:
Chem. modification of primary amino groups of mitochondrial membrane proteins by the fluorescent probe, fluorescamine (I), induced nonspecific membrane permeabilization. Titration of the Lys ε-amino group promoted efflux of accumulated Ca2+, collapse of transmembrane potential, and mitochondrial swelling. Ca2+ release was inhibited by cyclosporin A. Considering the latter, the authors assumed that I induced the permeability transition. Carboxyatractyloside also inhibited the reaction. Using a polyclonal antibody for adenine nucleotide translocase, Western blot anal. showed that the carrier appeared labeled with I. The results pointed out the importance of the ε-amino group of Lys residues, located in the adenine nucleotide carrier, on the modulation of membrane permeability, since its blockage suffices to promote opening of the nonspecific nanopore. In the experiment, the researchers used many compounds, for example, 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9HPLC of Formula: 38183-12-9).
4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9) belongs to benzofurans derivatives. Benzofurans are only weakly aromatic in nature and they are cleaved by many oxidative and reductive conditions. As benzofurans are prone to undergo ring opening of the heterocycle, examples of reduction of this type of aromatics by using dissolving metals are rather scarce.HPLC of Formula: 38183-12-9
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem