A mix-and-click method to measure amyloid-β concentration with sub-micromolar sensitivity was written by Xue, Christine;Lee, Yoon Kyung;Tran, Joyce;Chang, Dennis;Guo, Zhefeng. And the article was included in Royal Society Open Science in 2017.Formula: C17H10O4 This article mentions the following:
Aggregation of amyloid-β (Aβ) protein plays a central role in Alzheimer’s disease. Because protein aggregation is a concentration-dependent process, rigorous investigations require accurate concentration measurements. Owing to the high aggregation propensity of Aβ protein, working solutions of Aβ are typically in the low micromolar range. Therefore, an ideal Aβ quantification method requires high sensitivity without sacrificing speed and accuracy. Absorbance at 280nm is frequently used to measure Aβ concentration, but the sensitivity is low with only one tyrosine and no tryptophan residues in the Aβ sequence. Here we present a fluorescence method for Aβ quantification using fluorescamine, which gives high fluorescence upon reaction with primary amines.We show that, using hen egg white lysozyme as a standard, fluorescence correlates linearly with primary amine concentration across a wide range of fluorescamine concentrations, from 62.5 to 1000μM. The maximal sensitivity of detection is achieved at a fluorescamine concentration of 250μM or higher. The fluorescamine method is compatible with the presence of DMSO, which is commonly used in the preparation of Aβ oligomers, and limits the use of absorbance at 280nm due to its high background reading. Using aggregation kinetics, we show that the fluorescaminemethod gives accurate concentration measurements at low micromolar range and leads to highly consistent aggregation data. We recommend the fluorescamine assay to be used for routine and on-thefly concentration determination in Aβ oligomerization and fibrillization experiments In the experiment, the researchers used many compounds, for example, 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9Formula: C17H10O4).
4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9) belongs to benzofurans derivatives. Benzofuran derivatives are one of the most important oxygen-containing heterocycles. Benzofurans have also made significant and distinctive contributions to biology. They exhibit several biological activities that range from antioxidant, antitubercular, antiplasmodial, insecticidal.Formula: C17H10O4
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem