Determination of trifolirhizin in Sophora flavescens Ait. by HPLC was written by Zhao, Yuezhen;Xiang, Daxiong. And the article was included in Zhongnan Yaoxue in 2008.COA of Formula: C22H22O10 This article mentions the following:
An HPLC method for the determination of trifolirhizin in Sophora flavescens Ait. was established. A column of HYPERSIL BDS C18 (4.6 mm × 200 mm, 5 μm) was used. The mobile phase was composed of methanol and 0.2% phosphoric acid with a linear gradient elution from 10% to 40% methanol in 20 min. The flow rate was 1.0 mL/min-1, and the detection wavelength was 310 nm. The calibration curve of trifolirhizin was in good linearity over the range of 0.446-8.920 μg, (r = 0.9999). The average recovery was 97.94%, and RSD was less than 1% (n = 6). The method is simple, sensitive, accurate, reproducible, and can be used to control the quality of Sophora flavescens Ait. In the experiment, the researchers used many compounds, for example, (2S,3R,4S,5S,6R)-2-(((6aR,12aR)-6a,12a-Dihydro-6H-[1,3]dioxolo[4′,5′:5,6]benzofuro[3,2-c]chromen-3-yl)oxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol (cas: 6807-83-6COA of Formula: C22H22O10).
(2S,3R,4S,5S,6R)-2-(((6aR,12aR)-6a,12a-Dihydro-6H-[1,3]dioxolo[4′,5′:5,6]benzofuro[3,2-c]chromen-3-yl)oxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol (cas: 6807-83-6) belongs to benzofurans derivatives. Benzofurans are compounds with a planar structure having 10 pi electrons that include the lone pair on oxygen atom, which makes it more susceptible to electrophilic attack. As benzofurans are prone to undergo ring opening of the heterocycle, examples of reduction of this type of aromatics by using dissolving metals are rather scarce.COA of Formula: C22H22O10
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem