One- and Two-dimensional High-performance Thin-layer Chromatography as an Alternative Analytical Tool for Investigating Polyphenol-Protein Interactions was written by Tscherch, Kathrin;Biller, Julia;Lehmann, Mareen;Trusch, Maria;Rohn, Sascha. And the article was included in Phytochemical Analysis in 2013.COA of Formula: C17H10O4 This article mentions the following:
IntroductionPolyphenols and simple phenolic compounds are able to react with other food constituents during processing and storage. In the past, it has been shown that their reaction with proteins can lead to changes of the technofunctional or even physiol. properties of both compound classes. However, identification of specific binding sites of small mols. within a protein sequence and the corresponding conformational position is still challenging. ObjectiveInvestigating the reaction between different food proteins and phenolic compounds in alk. medium with one- and two-dimensional high-performance thin-layer chromatog. HPTLC coupled to matrix-assisted laser desorption/ionisation MALDI with time-of-flight TOF MS for analyzing the peptide profiles after tryptic digestion. Methods: After modification with phenolic compounds, protein derivatives were digested and peptides were separated with one- and two-dimensional HPTLC. Peptide profiles were detected with visible and UV wavelengths as well as with fluorescamine, ninhydrin and 2,2′-azino-bis3-ethylbenzothiazoline-6-sulfonic acid staining. In order to perform mass spectrometric measurements, peptides separated in the first dimension were analyzed by MALDI/TOF/MS. Results: Results show that the phenolic acids applied in this study show different specificity and susceptibility when modifying proteins resulting in changes of the peptide profiles, peptide quantity, polarity, UV-activity, radical-scavenging activity and mol. mass. ConclusionOne- and two-dimensional HPTLC supported by mass spectrometric detection represents an innovative, alternative tool for investigating and understanding polyphenol-protein interactions. This approach enables the identification of binding sites inside the protein chain and contributes to understanding the mechanism of polyphenol-protein interactions in vitro and in vivo. In the experiment, the researchers used many compounds, for example, 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9COA of Formula: C17H10O4).
4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9) belongs to benzofurans derivatives. Benzofurans are compounds with a planar structure having 10 pi electrons that include the lone pair on oxygen atom, which makes it more susceptible to electrophilic attack. Introduction of benzofurans in organic synthesis, particularly drug synthesis, involves generally the use of their metalated species as nucleophiles in addition reactions or in metal-catalysed cross-coupling reactions.COA of Formula: C17H10O4
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem