The three-dimensional configuration of the ester heterocycle is basically the same as that of the carbocycle. Compound: (R)-Ethyl 4-chloro-3-hydroxybutanoate(SMILESS: O=C(OCC)C[C@@H](O)CCl,cas:90866-33-4) is researched.Safety of Tri-n-octylphosphine Oxide. The article 《Yeast catalyzed reduction of β-keto esters (1): Factors affecting whole-cell catalytic activity and stereoselectivity》 in relation to this compound, is published in Biocatalysis and Biotransformation. Let’s take a look at the latest research on this compound (cas:90866-33-4).
Six yeasts were studied for their ability to reduce Et 4-chloroacetoacetate (Et 4-chloro-3-oxobutanoate) stereoselectively. Five species reduced the substrate to Et (S)-4-chloro-3-hydroxybutanoate of high (92-99%) optical purity. With glucose-grown cells, substrate reduction could only be demonstrated when growth was oxygen-limited, whereas xylose-grown Pichia capsulata could be grown under conditions of oxygen excess without losing its reducing ability. Zygosaccharomyces rouxii exhibited high enantioselectivity (≥98% ee (S)-enantiomer) under all conditions tested, while in P. capsulata, a novel switch was observed from producing mainly the (S)-enantiomer using glucose as co-substrate to producing mainly the (R)-enantiomer using 2-propanol as co-substrate. This switch was correlated with a change in reduction predominantly from an NADPH-dependent dehydrogenase system to an NADH-dependent system. In the production of Et (R)-4-chloro-3-hydroxybutanoate with P. capsulata, the enantioselectivity was also found to depend upon growth conditions. With glucose-grown cells, higher enantioselectivity was observed using cells harvested in stationary phase (93-94% ee) compared with cells harvested in exponential phase (43-60% ee). Growing P. capsulata with xylose rather than glucose as the major source of carbon for growth resulted in an eight-fold increase in the specific rate of Et (R)-4-chloro-3-hydroxybutanoate production using 2-propanol as co-substrate, although enantioselectivity was slightly reduced (65-81% ee) compared with the maximum achieved with glucose-grown cells. The effect of growth on xylose could also be correlated with enhanced activity of an NADH-dependent (R)-selective dehydrogenase system.
From this literature《Yeast catalyzed reduction of β-keto esters (1): Factors affecting whole-cell catalytic activity and stereoselectivity》,we know some information about this compound(90866-33-4)COA of Formula: C6H11ClO3, but this is not all information, there are many literatures related to this compound(90866-33-4).
Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem