Quantification of Free Polyelectrolytes Present in Colloidal Suspension, Revealing a Source of Toxic Responses for Polyelectrolyte-Wrapped Gold Nanoparticles was written by Qiu, Tian A.;Torelli, Marco D.;Vartanian, Ariane M.;Rackstraw, Nathan B.;Buchman, Joseph T.;Jacob, Lisa M.;Murphy, Catherine J.;Hamers, Robert J.;Haynes, Christy L.. And the article was included in Analytical Chemistry (Washington, DC, United States) in 2017.Application of 38183-12-9 This article mentions the following:
Polyelectrolyte (PE) wrapping of colloidal nanoparticles (NPs) is a standard method to control NP surface chem. and charge. As excess polyelectrolytes are usually employed in the surface modification process, it is critical to evaluate different purification strategies to obtain a clean final product and thus avoid ambiguities in the source of effects on biol. systems. In this work, 4-nm-diameter gold nanoparticles (AuNPs) were wrapped with 15 kDa poly(allylamine hydrochloride) (PAH), and three purification strategies were applied: (a) diafiltration or either (b) one round or (c) two rounds of centrifugation. The bacterial toxicity of each of these three PAH-AuNP samples was evaluated for the bacterium Shewanella oneidensis MR-1 and is quant. correlated with the amount of unbound PAH mols. in the AuNP suspensions, as judged by XPS and NMR experiments Dialysis experiments show that for a 15 kDa polyelectrolyte, a 50 kDa dialysis membrane is not sufficient to remove all unbound PAH polymers from a nanoparticle suspension. Together, these data showcase the importance of choosing a proper post-synthesis purification method for polyelectrolyte-wrapped NPs and reveal that apparent toxicity results may be due to unintended free wrapping agents like polyelectrolytes. In the experiment, the researchers used many compounds, for example, 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9Application of 38183-12-9).
4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9) belongs to benzofurans derivatives. Benzofuran derivatives are one of the most important oxygen-containing heterocycles. Benzofurans have also made significant and distinctive contributions to biology. They exhibit several biological activities that range from antiviral, antimicrobial, antitumor, anti-inflammatory.Application of 38183-12-9
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem