Nowakowska, Danuta et al. published their research in Experimental and Therapeutic Medicine in 2020 | CAS: 76-54-0

2′,7′-Dichloro-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 76-54-0) belongs to benzofurans derivatives. Benzofurans are compounds with a planar structure having 10 pi electrons that include the lone pair on oxygen atom, which makes it more susceptible to electrophilic attack. Introduction of benzofurans in organic synthesis, particularly drug synthesis, involves generally the use of their metalated species as nucleophiles in addition reactions or in metal-catalysed cross-coupling reactions.Application of 76-54-0

In vitro effects of vasoconstrictive retraction agents on primary human gingival fibroblasts was written by Nowakowska, Danuta;Saczko, Jolanta;Szewczyk, Anna;Michel, Olga;Zietek, Marek;Wezgowiec, Joanna;Wieckiewicz, Wlodzimierz;Kulbacka, Julita. And the article was included in Experimental and Therapeutic Medicine in 2020.Application of 76-54-0 This article mentions the following:

In the present study, we aimed to evaluate the in vitro effects of vasoconstrictive retraction agents on primary human gingival fibroblasts (HGFs). A total of six com. adrenergic solutions (0.05 and 0.01% HCl-epinephrine, two based on 0.05% HCl-tetrahydrozoline, 0.05% HCl-oxymetazoline, and 10% HCl-phenylephrine) and three exptl. gel formulations (EG-1, EG-2, and EG-3) were used to treat primary HGFs. The biol. effect of the retraction treatment on the expression of collagen types I and III was detected by performing immunocytochem. anal. The generation of reactive oxygen species triggered by the retraction agents were evaluated by using the dichlorofluorescein (DCF) fluorescent probe. The effect of retraction agents on the expression of fibronectin was visualized by confocal laser scanning microscopy. According to the results, exptl. retraction gels did not limit the expression of collagen types I and III. EG-3 even induced the synthesis of both types of collagen. The DCF assay indicated oxidative stress similar to the control cells for most of the selected retraction agents. Exptl. gels did not cause degradation of the cellular shape and morphol. of the primary HGFs. The proposed exptl. retraction gels in the present study demonstrated higher biocompatibility with primary HGFs, suggesting their use as clin. vasoconstrictive agents for the application of gingival retraction with minimal damage to periodontal tissues. In the experiment, the researchers used many compounds, for example, 2′,7′-Dichloro-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 76-54-0Application of 76-54-0).

2′,7′-Dichloro-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 76-54-0) belongs to benzofurans derivatives. Benzofurans are compounds with a planar structure having 10 pi electrons that include the lone pair on oxygen atom, which makes it more susceptible to electrophilic attack. Introduction of benzofurans in organic synthesis, particularly drug synthesis, involves generally the use of their metalated species as nucleophiles in addition reactions or in metal-catalysed cross-coupling reactions.Application of 76-54-0

Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem