The preparation of ester heterocycles mostly uses heteroatoms as nucleophilic sites, which are achieved by intramolecular substitution or addition reactions. Compound: (R)-Ethyl 4-chloro-3-hydroxybutanoate( cas:90866-33-4 ) is researched.Related Products of 90866-33-4.Ni, Yan; Li, Chun-Xiu; Ma, Hong-Min; Zhang, Jie; Xu, Jian-He published the article 《Biocatalytic properties of a recombinant aldo-keto reductase with broad substrate spectrum and excellent stereoselectivity》 about this compound( cas:90866-33-4 ) in Applied Microbiology and Biotechnology. Keywords: biocatalysis aldo keto reductase substrate spectrum enantiomer stereoselectivity. Let’s learn more about this compound (cas:90866-33-4).
In the screening of 11 E. coli strains overexpressing recombinant oxidoreductases from Bacillus sp. ECU0013, an NADPH-dependent aldo-keto reductase (YtbE) was identified with capability of producing chiral alcs. The protein (YtbE) was overexpressed, purified to homogeneity, and characterized of biocatalytic properties. The purified enzyme exhibited the highest activity at 50°C and optimal pH at 6.5. YtbE served as a versatile reductase showing a broad substrate spectrum towards different aromatic ketones and keto esters. Furthermore, a variety of carbonyl substrates were asym. reduced by the purified enzyme with an addnl. coupled NADPH regeneration system. The reduction system exhibited excellent enantioselectivity (>99% ee) in the reduction of all the aromatic ketones and high to moderate enantioselectivity in the reduction of α-keto esters and β-keto esters. Among the ketones tested, Et 4,4,4-trifluoroacetoacetate was found to be reduced to Et (R)-4,4,4-trifluoro-3-hydroxy butanoate, an important pharmaceutical intermediate, in excellent optical purity. To the best of our knowledge, this is the first report of ytbE gene-encoding recombinant aldo-keto reductase from Bacillus sp. used as biocatalyst for stereoselective reduction of carbonyl compounds This study provides a useful guidance for further application of this enzyme in the asym. synthesis of chiral alc. enantiomers.
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Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem