Name: (R)-Ethyl 4-chloro-3-hydroxybutanoate. The protonation of heteroatoms in aromatic heterocycles can be divided into two categories: lone pairs of electrons are in the aromatic ring conjugated system; and lone pairs of electrons do not participate. Compound: (R)-Ethyl 4-chloro-3-hydroxybutanoate, is researched, Molecular C6H11ClO3, CAS is 90866-33-4, about Asymmetric reduction of ethyl 4-chloroacetoacetate to R-(+)-4-chloro-3-hydroxybutyrate by recombinant Escherichia coli strains. Author is Jing, Li; Lin, Jianping; Xu, Zhinan; Tan, Tianwei; Cen, Peilin.
NADP-dependent aldehyde reductase gene ALR and glucose-6-phosphate dehydrogenase gene gdh223 were cloned from Sporobolomyces salmonicolor and Bacillus megaterium ZJU0310 resp. in Escherichia coli. The recombinant E. coli M15 (pQE30-ALRgdh223) strain was and optimized by investigating the effects of the time of adding isopropylthiogalactoside (IPTG), IPTG concentration and temperature on enzyme activity. Experiment results showed that optimized conditions were IPTG concentration 0.4 mmol/L, 32 °C, 8 h. Under optimized conditions, 5.27 U/mg-protein of enzyme activity was obtained after 8 h cultivation. The asym. reduction of Et 4-chloroacetoacetate to R-(+)-4-chloro-3-hydroxybutyrate was carried out and it was found that the recombinant Escherichia Coli strain could regenerate coenzymes and increase ee. When reaction temperature was increased, product yield was increased, but over 32 °C, product yield was decreased. The reactant had an inhibiting effect on the reaction. The inhibition could be weakened and product yield could be increased by a fed-batch approach.
When you point to this article, it is believed that you are also very interested in this compound(90866-33-4)Name: (R)-Ethyl 4-chloro-3-hydroxybutanoate and due to space limitations, I can only present the most important information.
Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem