Colmenares-Cruz, Stephania; Gonzalez-Cortazar, Manases; Castaneda-Ramirez, Gloria Sarahi; Andrade-Gallegos, Rene H.; Sanchez, Jose E.; Aguilar-Marcelino, Liliana published the artcile< Nematocidal activity of hydroalcoholic extracts of spent substrate of Pleurotus djamor on L3 larvae of Haemonchus contortus>, Related Products of 531-88-4, the main research area is Haemonchus contortus Pleurotus djamor hydroalcoholic extract nematocidal activity; Biodirected fractionation; Haemonchus contortus; Harvest time; Nematocidal compounds; Nematocidal extracts.
The objective of this study was to evaluate and compare the in vitro lethal effect of the hydroalcoholic extract of the spent substrate of Pleurotus djamor ECS-123, obtained at 15 days of colonization (SPS) and at the first (SPS1) and second (SPS2) harvests, against infective larvae L3 of Haemonchus contortus. The in vitro lethal effect was evaluated by the L3 larval mortality test (LM) using six concentrations: 1.25, 2.5, 5, 10, 20, and 40 mg/mL, with ivermectin and thiabendazole (5 mg/mL) as controls. The first harvest extract (SPS1) of strain ECS-123 was subjected to liquid-liquid bipartition, which resulted in two fractions: aqueous (PdAcO) and Et acetate (PdAct). The chem. fractionation of PdAct with the highest mortality rate (80.11%) was carried out with open-column chromatog., giving a total of 13 fractions, which were analyzed by thin-layer chromatog. (TLC) and grouped into 5 mixtures (R1;1-3, R2;4-7, R3;8-9, R4;10-11 and R5;12-13). Subsequently, the mixtures were evaluated against H. contortus L3 larvae. Finally, the components of the mixtures with the highest nematocidal effects were evaluated by gas chromatog. coupled to mass spectrometry (GC-MS). The data were analyzed with a completely randomized design through ANOVA using the generalized linear model (GLM) with the quotRquot program. The purification and characterization of R4 and R5 by GC-MS revealed the presence of the following compounds: veratryl alc., 4-hydroxy-3,5,5 trimethyl-4-[3-oxo-1-butenyl]-2- cyclohexen-1-one, caffeine and 5,6-dimethoxy-1(3 H) isobenzofuranone. This information allowed for the identification of nematocidal compounds in the degraded substrate of P. djamor, an activity that had not been reported previously.
Veterinary Parasitology published new progress about Aqueous solutions. 531-88-4 belongs to class benzofurans, and the molecular formula is C10H10O4, Related Products of 531-88-4.
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem