Category: benzofurans

So far, in addition to halogen atoms, other non-metallic atoms can become part of the aromatic heterocycle, and the target ring system is still aromatic.Jing, Keju; Xu, Zhinan; Liu, Ying; Jiang, Xiaoxia; Peng, Li; Cen, Peilin researched the compound: (R)-Ethyl 4-chloro-3-hydroxybutanoate( cas:90866-33-4 ).Reference of (R)-Ethyl 4-chloro-3-hydroxybutanoate.They published the article 《Efficient production of recombinant aldehyde reductase and its application for asymmetric reduction of ethyl 4-chloro-3-oxobutanoate to ethyl (R)-4-chloro-3-hydroxybutanoate》 about this compound( cas:90866-33-4 ) in Preparative Biochemistry & Biotechnology. Keywords: recombinant aldehyde reductase asym reduction. We’ll tell you more about this compound (cas:90866-33-4).

An NADPH-dependent aldehyde reductase (ALR, EC1.1.1.2) gene is cloned from Sporobolomyces salmonicolor ZJUB 105, and inserted into plasmid pQE30 to construct the expression plasmid (pQE30-ALR). A variety of E. coli strains were employed as hosts to obtain transformants with pQE30-ALR, resp. Among these different types of transformants, the highest enzyme activity of ALR can be produced with E. coli M15 (pQE30-ALR). The bioactivity of ALR could be further improved significantly by the optimization of induction conditions. The results showed that the enzyme activity of ALR reached 6.48 U/mg protein, which is fifteen times higher than that of S. salmonicolor ZJUB 105. This recombinant strain was applied to the asym. reduction of Et 4-chloro-3-oxobutanoate (COBE) to Et (R)-4-chloro-3- hydroxybutanoate (CHBE). The results showed that the yield and optical purity of (R)-CHBE reached 98.5% and 99% e.e. (enantiomeric excess), resp.

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Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Zhang, Juan; Xia, Wen-Le; Ahmad, Fazal published an article about the compound: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate( cas:70539-42-3,SMILESS:O=C(ON1C(CCC1=O)=O)CCC(OCCOC(CCC(ON2C(CCC2=O)=O)=O)=O)=O ).Electric Literature of C18H20N2O12. Aromatic heterocyclic compounds can be classified according to the number of heteroatoms or the size of the ring. The authors also want to convey more information about this compound (cas:70539-42-3) through the article.

When 3T3-L1 fibroblasts differentiate to adipocytes, the specific activity of pyruvate carboxylase (PC) increases about 25-fold in parallel with its intracellular protein concentration The increase in PC protein concentration is accompanied by a 9-10-fold increase in the relative abundance of 4.2 kb PC mRNA measured by Northern-blot anal. using a cDNA probe encoding a segment of the PC gene of 3T3-L1 adipocytes. The effects of cAMP alone and together with insulin on levels of cellular protein, PC activity, PC protein and on the relative abundance of PC mRNA were examined in mature 3T3-L1 adipocytes. Adipocytes exposed to cAMP for 24 h exhibited a 25% decrease in cellular protein and marked decreases in enzyme activity (88%) and PC mRNA abundance (98%) compared with untreated adipocyte controls. After 48 h of exposure to cAMP, PC activity and PC mRNA diminished to levels approaching their detection limits. When exposed to medium containing cAMP plus insulin, adipocyte enzyme activity and PC mRNA declined more slowly during the first 24 h exposure (about 20% decrease) but after 48 h fell to values comparable with those of adipocytes exposed to cAMP alone. Despite these decreases in enzyme activity, the PC protein content of adipocytes treated with cAMP alone or cAMP plus insulin are nearly identical with that of control adipocytes. The inactivation of PC in cAMP-treated adipocytes does not involve loss of the prosthetic groups from the holoenzyme. Crosslinking experiments suggest that the spatial arrangement of protomers in inactive PC may differ from that in the active tetrameric enzyme. Data presented suggest that, in addition to inducing inactivation, cAMP may also regulate adipocyte PC by decreasing transcription of the PC gene and/or enhancing the rate of degradation of PC mRNA.

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Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Epoxy compounds usually have stronger nucleophilic ability, because the alkyl group on the oxygen atom makes the bond angle smaller, which makes the lone pair of electrons react more dissimilarly with the electron-deficient system. Compound: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, is researched, Molecular C18H20N2O12, CAS is 70539-42-3, about Poly(3-hydroxyoctanoate), a promising new material for cardiac tissue engineering.HPLC of Formula: 70539-42-3.

Cardiac tissue engineering (CTE) is currently a prime focus of research because of an enormous clin. need. In the present work, a novel functional material, poly(3-hydroxyoctanoate), P(3HO), a medium chain-length polyhydroxyalkanoate (PHA), produced using bacterial fermentation, was studied as a new potential material for CTE. Engineered constructs with improved mech. properties, crucial for supporting the organ during new tissue regeneration, and enhanced surface topog., to allow efficient cell adhesion and proliferation, were fabricated. Results showed that the mech. properties of the final patches were close to that of cardiac muscle. Biocompatibility of neat P(3HO) patches, assessed using neonatal ventricular rat myocytes (NVRM), showed that the polymer was as good as collagen in terms of cell viability, proliferation and adhesion. Enhanced cell adhesion and proliferation prop. were observed when porous and fibrous structures were incorporated into the patches. In addition, no deleterious effect was observed on adult cardiomyocyte contraction when cardiomyocytes were seeded on the P(3HO) patches. Hence, P(3HO)-based multifunctional cardiac patches are promising constructs for efficient CTE. This work will have a pos. impact on the development of P(3HO) and other PHAs as a novel new family of biodegradable functional materials with huge potential in a range of different biomedical applications, particularly CTE, leading to further interest and exploitation of these materials.

There is still a lot of research devoted to this compound(SMILES：O=C(ON1C(CCC1=O)=O)CCC(OCCOC(CCC(ON2C(CCC2=O)=O)=O)=O)=O)HPLC of Formula: 70539-42-3, and with the development of science, more effects of this compound(70539-42-3) can be discovered.

Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

The chemical properties of alicyclic heterocycles are similar to those of the corresponding chain compounds. Compound: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, is researched, Molecular C18H20N2O12, CAS is 70539-42-3, about Cross-linked enzyme aggregates (CLEAs) of Penicillium notatum lipase enzyme with improved activity, stability and reusability characteristics, the main research direction is Penicillium lipase immobilization cross linked enzyme aggregate EGNHS; ethylene glycol bissuccinimidoylsuccinate CLEA lipase thermostability activity; Activity recovery; Cross-linked enzyme aggregates; Lipase; Pencillium notatum; Reusability; Thermo-stability.Computed Properties of C18H20N2O12.

Cross-linked enzyme aggregates (CLEAs) are considered as an effective tool for the immobilization of enzyme. In this study, Pencillium notatum lipase (PNL) was immobilized as carrier free cross-linked enzyme aggregates using glutaraldehyde (GLA) and Ethylene glycol-bis [succinic acid N-hydroxysuccinimide] (EG-NHS) as crosslinking agents. The optimal conditions for the synthesis of an efficient lipase CLEAs such as precipitant type, the nature and amount of crosslinking reagent, and crosslinking time were optimized. The recovered activities of CLEAs were considerably dependent on the concentration of GLA; however, the activity recovery was not severely affected by EG-NHS as a mild cross-linker. The EG-NHS aggregates displayed superior hydrolytic (52.08 ± 2.52%) and esterification (64.42%) activities as compared to GLA aggregates which showed 23.8 ± 1.86 and 34.54% of hydrolytic and esterification activity, resp. Morphol. anal. by fluorescence and scanning electron microscope revealed that EG-NHS aggregates were smaller in size with larger surface area compared to GLA aggregates. The pH optima of both types of CLEAs were displaced to slightly alk. region and higher temperature as compared to native enzyme. Highest enzyme activity of CLEAs was achieved at the pH of 9.0 and 42 °C temperature Moreover, a significant improvement in the thermal resistance was also recorded after immobilization. After ten reusability cycles in aqueous medium, GLA and EG-NHS cross-linked lipase CLEAs preserved 63.62% and 70.9% of their original activities, resp. The results suggest that this novel CLEA-lipase is potentially usable in many industrial applications.

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Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Heterocyclic compounds can be divided into two categories: alicyclic heterocycles and aromatic heterocycles. Compounds whose heterocycles in the molecular skeleton cannot reflect aromaticity are called alicyclic heterocyclic compounds. Compound: 2923-28-6, is researched, Molecular CAgF3O3S, about Structure of copper(I) and silver(I) complexes with zwitterionic ligands derived from N-Heterocyclic Carbenes, the main research direction is copper silver bisdiisopropylphenylimidazolylidene complex preparation; crystal structure copper silver bisdiisopropylphenylimidazolylidene complex.Electric Literature of CAgF3O3S.

N-Heterocyclic Carbene (NHC) ligands have been instrumental in the synthesis of novel metal and main group-containing compounds, as well as their subsequent reactivity. Here, the coordination chem. of CS2 and PhNCS derivatives of the sterically encumbering 1,3-bis(2,6-diisopropylphenyl)imidazol-2-ylidene (IPr) are studied. The reaction of IPr•CS2 with Cu(I) and Ag(I) salts results in the isolation of dinuclear complexes, [(IPr•CS2)2M2(THF)n][X]2, M = Cu, X = PF6, n = 4; M = Ag, X = OTf, n = 2. The 1:1 stoichiometric reaction of IPr•PhNCS with CuI results in to the formation of [(IPr•PhNCS)2Cu][CuI2]. All complexes were characterized by 1H, 13C, and IR spectroscopy and their solid-state structures determined by X-ray crystallog.

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Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

The three-dimensional configuration of the ester heterocycle is basically the same as that of the carbocycle. Compound: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate(SMILESS: O=C(ON1C(CCC1=O)=O)CCC(OCCOC(CCC(ON2C(CCC2=O)=O)=O)=O)=O,cas:70539-42-3) is researched.Application of 19777-66-3. The article 《The use of an in vitro sperm activation assay to detect chemically induced damage of human sperm nuclei》 in relation to this compound, is published in Reproductive Toxicology. Let’s take a look at the latest research on this compound (cas:70539-42-3).

We report that human sperm chem. damaged in vitro by treatment with a reversible crosslinker, ethylene glycol bis(sulfosuccinimidylsuccinate; SEGS), display abnormal chromatin decondensation when analyzed in the human sperm activation assay (HSAA). Less than 20% of SEGS-treated sperm fully decondensed, vs. 97% of control sperm. Chem. reversal of the crosslinks by treatment with 5 μM hydroxylamine restored full decondensation in 76% of treated sperm. These results demonstrate that chem. damaged sperm respond abnormally in the HSAA, and that chem. damage to sperm nuclei can be detected using the HSAA. Thus, there is potential for the HSAA to be used to detect chem. alterations of sperm nuclei from men exposed to environmental toxicants.

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Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Pawelczyk, Ewaryst; Wachowiak, Roman published an article about the compound: Sodium 4-butyl-3,5-dioxo-1,2-diphenylpyrazolidin-4-ide( cas:129-18-0,SMILESS:O=C(N(C1=CC=CC=C1)N2C3=CC=CC=C3)[C-](CCCC)C2=O.[Na+] ).Quality Control of Sodium 4-butyl-3,5-dioxo-1,2-diphenylpyrazolidin-4-ide. Aromatic heterocyclic compounds can be classified according to the number of heteroatoms or the size of the ring. The authors also want to convey more information about this compound (cas:129-18-0) through the article.

The decomposition products formed when 20% aqueous alk. solutions of phenylbutazone Na salt were aged in sunlight were isolated and identified. The colored decomposition products were isolated by CHCl3 extraction; the colorless products were extracted with ether from the acidified solution The extracts were concentrated and separated by thin-layer chromatog. The 5 decomposition products isolated and identified with Ce(SO4)2 were (Rfvalue given) 4-hydroxyphenylbutazone (0.33), N-(2-carboxycaproyl)-hydrazobenzene (0.1), N-(2-carboxy-2-hydroxycaproyl)hydrazobenzene (0.55), trans-azobenzene (0.78), and cis-azobenzene (0.62).

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Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Application of 90866-33-4. So far, in addition to halogen atoms, other non-metallic atoms can become part of the aromatic heterocycle, and the target ring system is still aromatic. Compound: (R)-Ethyl 4-chloro-3-hydroxybutanoate, is researched, Molecular C6H11ClO3, CAS is 90866-33-4, about Stereoselective reduction of ethyl 4-chloro-3-oxobutanoate by Escherichia coli transformant cells coexpressing the aldehyde reductase and glucose dehydrogenase genes.

The asym. reduction of Et 4-chloro-3-oxobutanoate to Et (R)-4-chloro-3-hydroxybutanoate (I) using E. coli cells, which coexpress both the aldehyde reductase gene from Sporobolomyces salmonicolor and the glucose dehydrogenase (GDH) gene from Bacillus megaterium as a catalyst was investigated. In an organic solvent-water 2-phase system, I formed in the organic phase amounted to 1610 mM (268 mg/mL), with a molar yield of 94.1% and an optical purity of 91.7% e.e. The calculated turnover number of NADP+ to I formed was 13,500 mol/mol. Since the use of E. coli JM109 cells harboring pKAR and pACGD as a catalyst is simple and does not require the addition of GDH or the isolation of the enzymes, it is highly advantageous for the practical synthesis of I.

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Benzofuran – Wikipedia,
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Quality Control of (R)-Ethyl 4-chloro-3-hydroxybutanoate. The reaction of aromatic heterocyclic molecules with protons is called protonation. Aromatic heterocycles are more basic than benzene due to the participation of heteroatoms. Compound: (R)-Ethyl 4-chloro-3-hydroxybutanoate, is researched, Molecular C6H11ClO3, CAS is 90866-33-4, about Synthesis of ethyl (R)-4-chloro-3-hydroxybutyrate by immobilized cells using amino acid-modified magnetic nanoparticles. Author is Lu, Yuan; Dai, Hongqian; Shi, Hanbing; Tang, Lan; Sun, Xingyuan; Ou, Zhimin.

Fe3O4-Arg was selected as the optimal carrier due to its high activity recovery of immobilized cells in the preparation of Fe3O4-Arg-Cells. The optimal immobilization conditions for the preparation of Fe3O4-Arg-Cells were 30°C, 4 h, pH 7, and 3 g dry yeast. The activity recovery of immobilized cells reached 76.8%. For a batch reduction in a shaker in an alternating magnetic field, Fe3O4-Arg-Cells were used as a catalyst to gain Et (R)-4-chloro-3-hydroxybutyrate ((R)-CHBE). For further improvement in reduction productivity, a continuous reduction in the magnetic fluidized bed reactor system (MFBRS) was completed. Under their optimal transformation conditions, it took 24 h for Fe3O4-Arg-Cells to complete the conversion of Et 4-chloro-3-oxobutanoate (COBE) (0.8553 mol/L) in the shaker and only 8 h for the batch reduction in an alternating magnetic field. Continuous reduction in MFBRS provided new ideas for the efficient production of (R)-CHBE; 1.5882 mol/L (10 mL) of COBE can be completely converted in 6 h. The conversion and enantiomeric excess (e.e.) of (R)-CHBE were 100% and above 99.9% resp., in the three reaction systems mentioned above.

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Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem

Category: benzofurans. The fused heterocycle is formed by combining a benzene ring with a single heterocycle, or two or more single heterocycles. Compound: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, is researched, Molecular C18H20N2O12, CAS is 70539-42-3, about Affinity labeling of the Fc receptor on human monocytes using bifunctional cross-linking agents. Author is Kurlander, Roger; Niedel, James.

To affinity label the Fc receptor on human monocytes, Fc fragments of monoclonal human IgG1 radiolabeled with 125I were covalently bound to the surface of intact monocytes using a variety of bifunctional cross-linking agents including ethylene glycol bis(succinimidyl succinate), dithio-bis-(succinimidyl proprionate), maleimidobenzoyl N-hydroxysuccinimide, glutaraldehyde, and di-Me suberimidate. After cross-linking, cells were solubilized and subjected to SDS-polyacrylamide gel electrophoresis, followed by radioautog. Each of these cross-linkers caused a portion of cell-bound Fc fragments to form a covalent complex with a monocyte membrane component. This complex migrated on electrophoresis with an apparent mol. weight of 120,000. Deducting the mol. weight of Fc fragments alone (53,000) the mol. weight of the second component of the complex therefore was about 67,000. A similar estimate of receptor size also was obtained after reduction with dithiothreitol. Complex formation was potently inhibited by unlabeled Fc fragments, IgG1 or IgG3, all of which would be expected to compete with Fc fragments for IgG Fc receptor on human monocytes; it was not inhibited by Fab fragments, IgG2 or IgG4, which do not bind avidly to this receptor. Complex formation was saturable, and Fc fragments formed complexes with avidity comparable to that with which Fc fragments bound to receptors on intact monocytes. The findings establish the feasibility of using radiolabeled Fc fragments to affinity label the IgG Fc receptors on human leukocytes. Potential advantages of this approach to studying receptor structure are discussed.

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Reference:
Benzofuran – Wikipedia,
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