The combination of HPLC and biological analysis to determine the quality markers and its structural composition of Eclipta prostrata L. was written by Wang, Long;Huang, Bin;Li, Chenzi;Yang, Bin;Jia, Xiaobin;Feng, Liang. And the article was included in Phytochemical Analysis in 2020.Quality Control of 1,8,9-Trihydroxy-3-methoxy-6H-benzofuro[3,2-c]chromen-6-one The following contents are mentioned in the article:
The diversity and complexity of components are important reasons for the unstable efficacy and safety of Chinese materia medica (CMM) in quality control. The good and stable quality control may be related to the quality markers with structural composition of multi-components. In the present study, we take Eclipta prostrata L. as a representative example. The 11 samples were collected from the different areas in China, and the discrepancy in bioactivity and chem. composition of these samples were compared. DOX (doxorubicin hydrochloride)-induced ICR mice were established for in vivo nephrotic syndrome experiments The biochem. indicators including 24-h urine protein, triglyceride (TG), etc. were measured and the pathol. change of kidney tissue was observed MPC-5 cells damage model was induced to compare the difference of these samples in bio-activity. High-performance liquid chromatog. (HPLC) method for 11 EEP (extract of Eclipta prostrata L.) samples were performed to analyze the content of the quality markers. In vivo experiments, EEP could mitigate the content or activity of urine protein, TG, etc. compared with the pos. group (TG content was 2.53 ± 0.39 mmol/L, urinary protein quantification on 35th day was 16.79 ± 2.32 mg). In vitro experiments, CCE (coumestans component of Ecliptae) and EEP had equivalent effects on biochem. indicators such as cell viability, etc. According to the HPLC anal., the content of wedelolactone was 45.88% and demethylwedelolactone was 23.74% in CCE. The CCE with a ratio of 2:1 can be considered as a quality marker of Eclipta prostrata L.. This research may provide a perspective for quality control of CMM. This study involved multiple reactions and reactants, such as 1,8,9-Trihydroxy-3-methoxy-6H-benzofuro[3,2-c]chromen-6-one (cas: 524-12-9Quality Control of 1,8,9-Trihydroxy-3-methoxy-6H-benzofuro[3,2-c]chromen-6-one).
1,8,9-Trihydroxy-3-methoxy-6H-benzofuro[3,2-c]chromen-6-one (cas: 524-12-9) belongs to benzofurans derivatives.Benzofuran is one of the most significant oxygen-containing heterocycles consisting of fused benzene and furan ring, which are widely presented in various naturally occurring and synthetically active compounds. Benzofurans have also made significant and distinctive contributions to biology. They exhibit several biological activities that range from antiviral, antimicrobial, antitumor, anti-inflammatory.Quality Control of 1,8,9-Trihydroxy-3-methoxy-6H-benzofuro[3,2-c]chromen-6-one
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem