In-Droplet Electrophoretic Separation and Enrichment of Biomolecules was written by Saucedo-Espinosa, Mario A.;Dittrich, Petra S.. And the article was included in Analytical Chemistry (Washington, DC, United States) in 2020.Related Products of 76-54-0 This article mentions the following:
The authors demonstrate the in-droplet separation and enrichment of mols. from small organic mols. to long nucleic acids (lambda DNA). Elec. potentials are applied via two parallel three-dimensional electrodes, which interface the nanodroplets through polydimethylsiloxane (PDMS)-carbon composite membranes. These membranes enable the generation of uniform elec. fields inside the droplets, while simultaneously preventing the formation of electrolytic byproducts. Biomols. of different sizes migrate toward one side of the droplets, according to their net charge, when exposed to the elec. field. Directly afterward, a Y-junction promotes droplet splitting, resulting in the generation of biomol.-enriched daughter droplets. Biomols. were fluorescently labeled, and fluorescence microscopy was employed to assess their electrophoretic separation and enrichment. Exptl. results demonstrate how the enrichment of biomols. is influenced by their size, charge, and concentration, by the ionic strength, viscosity, and pH of the suspending medium, and by the in-droplet flow profile. Enrichments above 95% were observed for small mols. and highly charged species at velocities over 10 mm/s (13 droplets per s). Moreover, the enrichment performance asymptotically approached a value of 38% for velocities as high as 50 mm/s, demonstrating the potential of this technique for the high-throughput separation of charged species. The applicability of the system was demonstrated by cleaving a peptide and selectively separating the cleaved fragments in different daughter droplets on the basis of their net charge. In the experiment, the researchers used many compounds, for example, 2′,7′-Dichloro-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 76-54-0Related Products of 76-54-0).
2′,7′-Dichloro-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 76-54-0) belongs to benzofurans derivatives. Many natural or synthetic compounds containing benzofuran skeletons have been found to possess remarkable activity as agrochemicals and pharmaceuticals. Substituted benzofurans find applications such as fluorescent sensors, oxidants, in drug discovery, and in another field of chemistry and agriculture.Related Products of 76-54-0
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem