Safety of (R)-Ethyl 4-chloro-3-hydroxybutanoate. The fused heterocycle is formed by combining a benzene ring with a single heterocycle, or two or more single heterocycles. Compound: (R)-Ethyl 4-chloro-3-hydroxybutanoate, is researched, Molecular C6H11ClO3, CAS is 90866-33-4, about Stereospecific Reduction of Methyl O-Chlorobenzoylformate at 300 g/L-1 without Additional Cofactor using a Carbonyl Reductase Mined from Candida glabrata. Author is Ma, Hongmin; Yang, Linlin; Ni, Yan; Zhang, Jie; Li, Chun-Xiu; Zheng, Gao-Wei; Yang, Huaiyu; Xu, Jian-He.
In order to search for oxidoreductases suitable for the preparation of Me (R)-O-chloromandelate [(R)-CMM], the key intermediate for clopidogrel, the homologous proteins of Gre2p were expressed in Escherichia coli, among which CgKR1 showed the most satisfactory activity and stereoselectivity towards Me o-chlorobenzoylformate (CBFM). Using the crude enzyme of CgKR1 and glucose dehydrogenase (GDH), as much as 300 g/L-1 of CBFM was almost stoichiometrically converted to (R)-CMM with excellent enantiomeric excess (98.7% ee). More importantly, the reaction could be performed without external addition of an expensive cofactor. The substrate profile indicates that keto esters serve as the most suitable substrate, which was confirmed by gram-scale preparations Homol. modeling and docking anal. revealed the mol. basis for the high stereoselectivity of CgKR1. These demonstrate not only the feasibility of in silico mining of novel enzymes based on sequence homol. but also the applicability of this new reductase for the practical production of optically active (R)-CMM.
This literature about this compound(90866-33-4)Safety of (R)-Ethyl 4-chloro-3-hydroxybutanoatehas given us a lot of inspiration, and I hope that the research on this compound((R)-Ethyl 4-chloro-3-hydroxybutanoate) can be further advanced. Maybe we can get more compounds in a similar way.
Reference:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem