Development, validation and application of RP-HPLC method for quantitative estimation of wedelolactone in different accessions and plant parts of Eclipta alba (L.) was written by Kumar, B. Anil;Rao, V. K.;Bindu, K. Hima;Rohini, M. R.;Shivakumar. And the article was included in Journal of Plant Biochemistry and Biotechnology.Name: 1,8,9-Trihydroxy-3-methoxy-6H-benzofuro[3,2-c]chromen-6-one The following contents are mentioned in the article:
In the current study, a simple, rapid reversed phase high performance liquid chromatog. (RP- HPLC) method was developed and validated for evaluation of wedelolactone in Eclipta alba, an indigenous medicinal herb commonly known as Bhringaraj. Wedelolactone was quantified in the dried root, stem, leaves, flowers and whole plant of E. alba by a gradient RP-HPLC procedure with buffer (1 mM potassium dihydrogen phosphate-KH2PO4) and acetonitrile as mobile phase and separation was carried out on CAPCELL PAK C18 (SHISEIDO) column at a flow rate of 1.5 mL/min and UV detection at 351 nm using photodiode array detector (PDA). The method was validated as per ICH Q2 (R1) (International Conference on Harmonization of Tech. Requirements for the Registration of Pharmaceuticals for Human Use) guidelines for various anal. method validation parameters such as linearity, accuracy, specificity, precision, and sensitivity (limit of detection and limit of quantification). The precision of the method was confirmed by a relative standard deviation < 2.0% (n = 6), and wedelolactone recovery was observed between 99.5 and 103.6%. The LOD was found to be 0.084 μg/mL and the LOQ was 0.25 μg/mL, and the response was linear from 2.5 to 140 μg/mL with excellent correlation coefficient (R2) of 0.9997. The study also revealed that variation for the content of wedelolactone among accessions and between different plant parts, the highest content is found in the leaves. The developed method is precise, sensitive, accurate, reproducible and can be used for the quant. estimation of wedelolactone in E. alba plant material. This study involved multiple reactions and reactants, such as 1,8,9-Trihydroxy-3-methoxy-6H-benzofuro[3,2-c]chromen-6-one (cas: 524-12-9Name: 1,8,9-Trihydroxy-3-methoxy-6H-benzofuro[3,2-c]chromen-6-one).
1,8,9-Trihydroxy-3-methoxy-6H-benzofuro[3,2-c]chromen-6-one (cas: 524-12-9) belongs to benzofurans derivatives. Benzofuran derivatives have shown many biological activities, including antifungal and antimicrobial properties, and acting as antagonists of H3 receptors and angiotensin II. In nature, benzofurans have occupied an important role among the plant phenols & several pharmacologically active compounds.Name: 1,8,9-Trihydroxy-3-methoxy-6H-benzofuro[3,2-c]chromen-6-one
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem