A novel spectrofluorimetric method for the determination of calcitonin in ampuls through derivatization with fluorescamine was written by Kocdan, Deniz;Basan, Hasan. And the article was included in Luminescence in 2013.Product Details of 38183-12-9 This article mentions the following:
In this study, a simple, sensitive, and selective spectrofluorimetric method was developed for the determination of salmon calcitonin (sCT) in ampuls. The method is based on the reaction between sCT and fluorescamine at pH 8.5 in borate buffer, resulting in a highly fluorescent derivative Fluorescence of derivatized sCT solutions was measured by setting the excitation and emission monochromators and slit widths to 390, 484, and 10 nm, resp. Several derivatization parameters were optimized. A calibration graph was constructed using standard solutions of the derivatized calcitonin in the range 0.5-6.0 μg/mL. Limit of detection and limit of quantification values were determined to be 0.124 and 0.372 μg/mL, resp. The proposed method was successfully applied for the determination of sCT in com. available ampuls. High recovery values (101.0-102.0%), and a low relative standard deviation (RSD %) value (5.3-5.4) proved the accuracy and precision of the proposed method. An isocratic reversed-phase high-performance liquid chromatog. (HPLC) method, as a reference, was also developed for the determination of sCT. A reversed-phase Nucleosil C18 column (250 mm × 4.6 mm i.d., 10 μm particle size, 120 Å pore size) was used and the detector was set at 210 nm. Statistical comparison of the results of the 2 methods showed clearly that there was no significant difference between them. Copyright © 2012 John Wiley & Sons, Ltd. In the experiment, the researchers used many compounds, for example, 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9Product Details of 38183-12-9).
4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9) belongs to benzofurans derivatives. Benzofuran is a core structural unit found in many naturally occurring compounds with multidirectional biological activities. Benzofurans are stable towards alkali and readily polymerize on treatment with sulfuric acid, due to which they are useful for the preparation of low cost chemically relatively inert resins.Product Details of 38183-12-9
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem