Systematic profiling of the multicomponents and authentication of Erzhi Pill by UHPLC/Q-Orbitrap-MS oriented rapid polarity-switching data-dependent acquisition and selective monitoring of the chemical markers deduced from fingerprint analysis was written by Jia, Li;Fu, Lingling;Wang, Xiaoyan;Yang, Wenzhi;Wang, Hongda;Zuo, Tiantian;Zhang, Chunxia;Hu, Ying;Gao, Xiumei;Han, Lifeng. And the article was included in Molecules in 2018.HPLC of Formula: 524-12-9 The following contents are mentioned in the article:
The anal. platform UHPLC/Q-Orbitrap-MS offers a solution to quality investigation of TCM with high definiteness. Using Erzhi Pill (EZP) as a case, we developed UHPLC/Q-Orbitrap-MS based approaches to achieve systematic multicomponent identification and rapid authentication. Comprehensive multicomponent characterization of EZP was performed by neg./pos. switching data-dependent high-energy collision-induced dissociation-MS2 (HCD-MS2) after 25 min chromatog. separation By reference compounds comparison, elemental composition anal., fragmentation pathways interpretation, and retrieval of an inhouse library, 366 compounds were separated and detected from EZP, and 96 thereof were structurally characterized. The fingerprints of two component drugs (Ligustri Lucidi Fructus, LLF; Ecliptae Herba, EH) for EZP were analyzed under the same LC-MS condition by full scan in neg. mode. In combination with currently available pharmacol. reports, eight compounds were deduced as the ‘identity markers’ of EZP. Selective ion monitoring (SIM) of eight marker compounds was conducted to authenticate six batches of EZP samples. Both LLF and EH could be detected from all EZP samples by analyzing the SIM spectra, which could indicate their authenticity. Conclusively, UHPLC/Q-Orbitrap-MS by rapid polarity switching could greatly expand the potency of untargeted profiling with high efficiency, and SIM of multiple chem. markers rendered a practical approach enabling the authentication of TCM formulas. This study involved multiple reactions and reactants, such as 1,8,9-Trihydroxy-3-methoxy-6H-benzofuro[3,2-c]chromen-6-one (cas: 524-12-9HPLC of Formula: 524-12-9).
1,8,9-Trihydroxy-3-methoxy-6H-benzofuro[3,2-c]chromen-6-one (cas: 524-12-9) belongs to benzofurans derivatives. Benzofurans are only weakly aromatic in nature and they are cleaved by many oxidative and reductive conditions. Benzofurans are stable towards alkali and readily polymerize on treatment with sulfuric acid, due to which they are useful for the preparation of low cost chemically relatively inert resins.HPLC of Formula: 524-12-9
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem