Development and characterization of soya phosphatidylcholine complex of coumestans from Eclipta alba for the effective management of hepatotoxicity was written by Jain, Abhishek Kumar;Jain, Anki;Jain, Ashish. And the article was included in International Journal of Pharmacognosy in 2019.HPLC of Formula: 524-12-9 The following contents are mentioned in the article:
It is known that Eclipta alba Hassk. (Family: Compositae) contains coumestans (Wedelolactone and di-Me wedelolactone) are used in liver disorders. The objective of the present investigation was to develop a novel formulation of these coumestans in combination with the soya phosphatidylcholine (PC), to overcome the limitation of absorption and to investigate the protective effect of the coumestans-phosphatidylcholine complex (C-PC) on carbon tetrachloride-induced acute liver damage in rats. Methanolic extract (ME) of the whole plant of Eclipta alba was fractionated with water and then with Et acetate. The Et acetate fraction of methanolic extract (EFME) contains coumestans which were characterized by chem. tests. EFME and PC prepared the C-PC. FT-IR and DSC spectroscopy characterized the C-PC. In-vitro drug release from EFME and C-PC through egg membrane was performed using UV-Visible spectrophotometer. The hepatoprotective activity of C-PC (Equivalent to 5.35 and 10.7 mg/kg body weight of EFME), ME 250 mg/kg and EFME 5.35 mg/kg were evaluated on various enzymes level. C-PC significantly provided better protection to the liver by restoring the enzyme levels of SGPT (Serum glutamate pyruvic transaminase), SGOT (Serum glutamate oxaloacetate transaminase), ALP (Alk. phosphatase) and total bilirubin on carbon tetrachloride (CCl4) induced liver damaged animals (P<0.001). Histopathol. studies were performed, and the results showed that the C-PC provided better protection to rat liver than ME and EFME at similar doses as well as shown significant regeneration of hepatocytes, central vein, intact cytoplasm and nucleus. This study involved multiple reactions and reactants, such as 1,8,9-Trihydroxy-3-methoxy-6H-benzofuro[3,2-c]chromen-6-one (cas: 524-12-9HPLC of Formula: 524-12-9).
1,8,9-Trihydroxy-3-methoxy-6H-benzofuro[3,2-c]chromen-6-one (cas: 524-12-9) belongs to benzofurans derivatives. Benzofuran derivatives have shown many biological activities, including antifungal and antimicrobial properties, and acting as antagonists of H3 receptors and angiotensin II. In nature, benzofurans have occupied an important role among the plant phenols & several pharmacologically active compounds.HPLC of Formula: 524-12-9
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem