Flow radiocytometry using droplet optofluidics was written by Ha, Byunghang;Kim, Tae Jin;Moon, Ejung;Giaccia, Amato J.;Pratx, Guillem. And the article was included in Biosensors & Bioelectronics in 2021.Reference of 76-54-0 This article mentions the following:
Flow-based cytometry methods are widely used to analyze heterogeneous cell populations. However, their use for small mol. studies remains limited due to bulky fluorescent labels that often interfere with biochem. activity in cells. In contrast, radiotracers require minimal modification of their target mols. and can track biochem. processes with negligible interference and high specificity. Here, we introduce flow radiocytometry (FRCM) that broadens the scope of current cytometry methods to include beta-emitting radiotracers as probes for single cell studies. FRCM uses droplet microfluidics and radiofluorogenesis to translate the radioactivity of single cells into a fluorescent signal that is then read out using a high-throughput optofluidic device. As a proof of concept, we quantitated [18F]fluorodeoxyglucose radiotracer uptake in single human breast cancer cells and successfully assessed the metabolic flux of glucose and its heterogeneity at the cellular level. We believe FRCM has potential applications ranging from anal. assays for cancer and other diseases to development of small-mol. drugs. In the experiment, the researchers used many compounds, for example, 2′,7′-Dichloro-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 76-54-0Reference of 76-54-0).
2′,7′-Dichloro-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (cas: 76-54-0) belongs to benzofurans derivatives. Benzofuran derivatives are one of the most important oxygen-containing heterocycles. Benzofurans have also made significant and distinctive contributions to biology. They exhibit several biological activities that range from antioxidant, antitubercular, antiplasmodial, insecticidal.Reference of 76-54-0
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem