High-Throughput Screening at the Membrane Interface Reveals Inhibitors of Amyloid-β was written by Cox, Sarah J.;Lam, Brian;Prasad, Ajay;Marietta, Hannah A.;Stander, Nicholas V.;Joel, Joseph G.;Sahoo, Bikash R.;Guo, Fucheng;Stoddard, Andrea K.;Ivanova, Magdalena I.;Ramamoorthy, Ayyalusamy. And the article was included in Biochemistry in 2020.Formula: C16H10O7 The following contents are mentioned in the article:
Aggregation and the formation of oligomeric intermediates of amyloid-β (Aβ) at the membrane interface of neuronal cells are implicated in the cellular toxicity and pathol. of Alzheimer’s disease. Small mol. compounds have been shown to suppress amyloid aggregation and cellular toxicity, but often the presence of a lipid membrane negates their activity. A high-throughput screen of 1800 small mols. was performed to search for membrane active inhibitors, and 21 primary hits were discovered. Through the use of fluorescence-based assays, transmission electron microscopy, and dot blot assays, the initial 21 primary hits were narrowed down to five lead compounds NMR and CD experiments were used for further confirmation of amyloid inhibition at the membrane interface and to obtain insights into the secondary structure of amyloid-β, while size exclusion chromatog. was used to characterize the size of Aβ species. Lastly, dye-leakage assays allowed us to understand how the addition of the five lead compounds affected amyloid-β’s ability to permeate the lipid bilayer. These results provide insights into small mols. that stabilize small amyloid species in the presence of membranes for the development of tool compounds for deeper investigations of these transient species. This study involved multiple reactions and reactants, such as 1,8,9-Trihydroxy-3-methoxy-6H-benzofuro[3,2-c]chromen-6-one (cas: 524-12-9Formula: C16H10O7).
1,8,9-Trihydroxy-3-methoxy-6H-benzofuro[3,2-c]chromen-6-one (cas: 524-12-9) belongs to benzofurans derivatives. Benzofuran is the “”parent”” of many related compounds with more complex structures. For example, psoralen is a benzofuran derivative that occurs in several plants. As benzofurans are prone to undergo ring opening of the heterocycle, examples of reduction of this type of aromatics by using dissolving metals are rather scarce.Formula: C16H10O7
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem