Determination of sitagliptin with fluorescamine in tablets and spiked serum samples by spectrofluorimetry and a degradation study was written by Caglar, Sena;Onal, Armagan;Toker, Sidika. And the article was included in Current Pharmaceutical Analysis in 2012.Synthetic Route of C17H10O4 This article mentions the following:
Two novel, simple, and rapid stability-indicating spectrofluorimetric methods were developed for the determination of sitagliptin in tablets and spiked serum samples. In the first method, sitagliptin’s natural fluorescence was measured at 353 nm after excitation at 259 nm. On the basis of the reaction between sitagliptin and fluorescamine the second method was developed in borate buffer solution of pH 9.0 and the fluorescence intensities of the derivatives were measured at 475 nm emission and 390 nm excitation wavelengths. The calibration curves were constructed in concentration range of 0.5-10.0 μg mL-1 and 0.2-1.4 μg mL-1 for the first and second method, resp. The developed methods were validated with respect to linearity, precision, sensitivity, accuracy and selectivity. The degradation behavior of the drug was investigated using the first method. The drug solution was subjected to neutral, acid and alkali hydrolysis, oxidation, thermal stress and exposured to the sunlight. The first method is proved to be selective and useful for the investigation of the stability of sitagliptin. The application of spiked serum samples was also analyzed by the second method. The developed methods were successfully applied for the determination of sitagliptin in tablets and spiked serum samples. In the experiment, the researchers used many compounds, for example, 4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9Synthetic Route of C17H10O4).
4-Phenyl-3H,3’H-spiro[furan-2,1′-isobenzofuran]-3,3′-dione (cas: 38183-12-9) belongs to benzofurans derivatives. Benzofurans are compounds with a planar structure having 10 pi electrons that include the lone pair on oxygen atom, which makes it more susceptible to electrophilic attack. Introduction of benzofurans in organic synthesis, particularly drug synthesis, involves generally the use of their metalated species as nucleophiles in addition reactions or in metal-catalysed cross-coupling reactions.Synthetic Route of C17H10O4
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem