Chemical fingerprinting and quantification of biomarker wedelolactone in different sources of “Bhringaraja” by high-performance thin-layer chromatography and method validation was written by Hullatti, Kirankumar;Rodrigues, Analee;Mannur, Vinod;Mastiholimath, Vinayak;Rasal, Vijay Kumar. And the article was included in Journal of Planar Chromatography–Modern TLC in 2016.Name: 1,8,9-Trihydroxy-3-methoxy-6H-benzofuro[3,2-c]chromen-6-one The following contents are mentioned in the article:
Two different botanical sources, Eclipta alba and Wedelia calendulacea are used as “Bhringaraja” in the Ayurvedic system of medicine. In the present study, an effort has been made to evaluate different sources by using high-performance thin-layer chromatog. (HPTLC) as an anal. tool. Wedelolactone, one of the primary constituents of these plants, was taken as the marker compound for the evaluation. An HPTLC method was developed and validated for the evaluation of different sources of “Bhringaraja”. The chromatog. system was developed using silica gel 60 F254 HPTLC plates with the mobile phase toluene-chloroform-Et alc.-formic acid (5:4:1:0.5, volume/volume). Linearity was found between the concentration ranges of 80 to 280 ng spot-1 (R2: 0.9994), and limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.36 ng spot-1 and 1.09 ng spot-1. The study has shown the presence of wedelolactone at the concentration of 0.26% weight/weight and 0.05% weight/weight in E. alba and W. calendulacea, resp., whereas it was absent in another closely related species, Wedelia trilobata. At the same time, all the three plants were subjected to evaluation of quality-control parameters as per the World Health Organization (WHO) guidelines. Certain parameters such as foaming index, total alkaloids, and total bitter principles were significantly different in the three plants. Hence, the present HPTLC method development, and the validation and evaluation of quality-control parameters would be helpful in the standardization of individual plants and their formulations. This study involved multiple reactions and reactants, such as 1,8,9-Trihydroxy-3-methoxy-6H-benzofuro[3,2-c]chromen-6-one (cas: 524-12-9Name: 1,8,9-Trihydroxy-3-methoxy-6H-benzofuro[3,2-c]chromen-6-one).
1,8,9-Trihydroxy-3-methoxy-6H-benzofuro[3,2-c]chromen-6-one (cas: 524-12-9) belongs to benzofurans derivatives.Benzofuran is one of the most significant oxygen-containing heterocycles consisting of fused benzene and furan ring, which are widely presented in various naturally occurring and synthetically active compounds. Introduction of benzofurans in organic synthesis, particularly drug synthesis, involves generally the use of their metalated species as nucleophiles in addition reactions or in metal-catalysed cross-coupling reactions.Name: 1,8,9-Trihydroxy-3-methoxy-6H-benzofuro[3,2-c]chromen-6-one
Referemce:
Benzofuran – Wikipedia,
Benzofuran | C8H6O – PubChem